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In Vitro Induction Of Crown Galls By Agrobacterium Tumefaciens Super Virulent Strain A281 (Ptibo 542) In Lentil (Lens Culinaris Medik.), Khalid Mahmood Khawar, Sebahatti̇n Özcan
In Vitro Induction Of Crown Galls By Agrobacterium Tumefaciens Super Virulent Strain A281 (Ptibo 542) In Lentil (Lens Culinaris Medik.), Khalid Mahmood Khawar, Sebahatti̇n Özcan
Turkish Journal of Botany
Twenty-one genotypes of lentils (Lens culinaris Medik.), obtained from different sources in Turkey and Pakistan, were included in the study. Seeds were germinated in MS medium for 6-10 days with a 16 h photoperiod at 24°C, from which leaf and stem explants were isolated for inoculation with super virulent strain A281 (pTiBo 542) :: pBI121.1 agropine/mannopine type of Agrobacterium tumefaciens. The binary plasmid pBI121.1 carried a GUS gene directed by CaMV 35S promoter in order to confirm transformation by GUS expression. Parameters recorded include percentage of tumour formation, tumour diameter, and tumour weight. Pul 11, Kışlık Yeşil 21, Akm 565 …