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Full-Text Articles in Life Sciences

Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg Jan 2014

Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg

The Journal of Undergraduate Research

Alfalfa (Medicago sativa L.) is a valuable crop worldwide. In addition to its use as forage, the nitrogen fixation capabilities of root nodules make it a valuable component for improving soil health. Unfortunately, the semi-arid region of the Northern Great Plains introduces a number of environmental stresses that are detrimental to stand establishment and persistence. The ability to produce adventitious shoots from roots is generally considered a favorable trait for increasing stress resistance. This study aimed to identify alfalfa populations possessing the ability to produce adventitious shoot from root segments. Seven Plant Introductions (PIs), selected from the National Plant …


Shoot Morphology Of Eleven Alfalfa Populations, Jessica A. Schmuck Jan 2012

Shoot Morphology Of Eleven Alfalfa Populations, Jessica A. Schmuck

The Journal of Undergraduate Research

Alfalfa (Medicago sativa L.) is a major component of feed for dairy and beef cattle and one of the most productive forage species in North America. Alfalfa has been planted on millions of acres. More than 100 varieties have been developed in North America over the past 100 years. However, historically, alfalfa persistence under grazing in semiarid rangeland has generally been poor. Recently, it was discovered that naturally-selected populations of predominantly yellow-flowered alfalfa have been proven to be adapted to rangelands of western South Dakota and adjacent areas. A study was initiated in May 2006 to evaluate persistence and vigor …


Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets Jan 2009

Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets

The Journal of Undergraduate Research

Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures …