Life Sciences Commons^™

Remote Estimation Of Nitrogen And Chlorophyll Contents In Maize At Leaf And Canopy Levels, Michael Schlemmer, Anatoly A. Gitelson, James S. Schepers, Richard B. Ferguson, Y. Peng, J. Shanahan, Donald Rundquist

Department of Agronomy and Horticulture: Faculty Publications

Leaf and canopy nitrogen (N) status relates strongly to leaf and canopy chlorophyll (Chl) content. Remote sensing is a tool that has the potential to assess N content at leaf, plant, field, regional and global scales. In this study, remote sensing techniques were applied to estimate N and Chl contents of irrigated maize (Zea mays L.) fertilized at five N rates. Leaf N and Chl contents were determined using the red-edge chlorophyll index with R2 of 0.74 and 0.94, respectively. Results showed that at the canopy level, Chl and N contents can be accurately retrieved using green and red-edge Chl …

Optimal Copper Supply Is Required For Normal Plant Iron Deficiency Responses, Brian M. Waters, Laura C. Armbrust

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) and copper (Cu) homeostasis are tightly linked across biology. Understanding crosstalk between Fe and Cu nutrition could lead to strategies for improved growth on soils with low or excess metals, with implications for agriculture and phytoremediation. Here, we show that Cu and Fe nutrition interact to increase or decrease Fe and/or Cu accumulation in leaves and Fe uptake processes. Leaf Cu concentration increased under low Fe supply, while high Cu lowered leaf Fe concentration. Ferric reductase activity, an indicator of Fe demand, was inhibited at insufficient or high Cu supply. Surprisingly, plants grown without Fe were more susceptible …

Evaluation Of Three Nest Searching Methods For Ring-Necked Pheasant, Jeffery W. Stackhouse, Kevin K. Sedivec, Benjamin A. Geaumont

The Prairie Naturalist

Ring-necked pheasant (Phasianus colchicus) are a highly sought after game bird and as such, much research has been conducted regarding their ecology (Warner 1981, Trautman 1982, Johnson and Knue 1989). Perhaps the most investigated aspect of pheasant ecology is the species’ reproductive season (e.g. Linder et al. 1960, Dumke and Pils 1979, Leif 1994) with many studies focusing on nesting habitat (Baskett 1947, Clark et al. 1999). Results from previous research have acknowledged the difficulty associated with locating pheasant nests in their preferred cover types (Hanson 1970, Evrard 2000). Studies of pheasant nesting ecology have been based on …

Salamander Colonization Of Chase Lake, Stutsman County, North Dakota, David M. Mushet, Kyle I. Mclean, Craig A. Stockwell

The Prairie Naturalist

Salt concentrations in lakes are dynamic. In the western United States, water diversions have caused significant declines in lake levels resulting in increased salinity, placing many aquatic species at risk (Galat and Robinson 1983, Beutel et al. 2001). Severe droughts can have similar effects on salt concentrations and aquatic communities (Swanson et al. 2003). Conversely, large inputs of water can dilute salt concentrations and contribute to community shifts (Euliss et al. 2004).

Chase Lake is a large, shallow, alkaline lake in east-central North Dakota most famously known for supporting one of the largest breeding colonies of the American white pelican …

How Important Is A Quality Manuscript Review?, Christopher N. Jacques

The Prairie Naturalist

Temperatures are finally starting to cool a bit (for those of us in the northern Great Plains region) and Thanksgiving break is just around the corner.

I offer my reflections in the spirit of encouraging each of you to take a break from your professional obligations and spend time with friends and family enjoying nature and everything it has to offer this fall.

A Northward Range Extension Of The Hispid Cotton Rat (Sigmodon Hispidus) In Missouri, Cody W. Thompson, Elmer J. Finck

The Prairie Naturalist

The hispid cotton rat (Sigmodon hispidus) is a widely distributed rodent with a geographic range extending from north-central Mexico to southern Nebraska and central Virginia and from southeastern Arizona east to Florida (Carleton et al. 1999, Peppers and Bradley 2000, Wilson and Reeder 2005) with isolated populations in Arizona (Bradley et al. 2012) and California (Clark 1972). Range expansions for the species have been well documented (Clark 1972, Farney 1975, Benedict et al. 2000, Wright et al. 2010, Wills et al. 2011). The majority of these range expansions have occurred across the Central Plains during the 20th century …

Use Of Seeded Exotic Grasslands By Wintering Birds, Andrew D. George, Timothy J. O'Connell, Karen R. Hickman, David M. Lesliee Jr.

The Prairie Naturalist

Despite widespread population declines of North American grassland birds, effects of anthropogenic disturbance on wintering habitat of this guild remain poorly understood. We compared avian abundance and habitat structure in fields planted to the exotic grass Old World bluestem (Bothriochloa ischaemum; OWB) to that in native mixed-grass prairie. During winters of 2007–2008 and 2008–2009, we conducted bird and vegetation surveys in six native grass and six OWB fields in Garfield, Grant, and Alfalfa counties, Oklahoma. We recorded 24 species of wintering birds in native fields and 14 species in OWB monocultures. While vegetation structure was similar between field …

Whooping Cranes Consume Plains Leopard Frogs At Migratory Stopover Sites In Nebraska, Keith Geluso, Brad T. Krohn, Mary J. Harner, Michael J. Assenmacher

The Prairie Naturalist

Whooping cranes (Grus americana) currently consist of a single, wild population that migrates annually from breeding grounds at Wood Buffalo National Park, Canada, to wintering grounds on and around the Aransas National Wildlife Refuge along the Texas coast, USA (NRC 2005). This population reached a low of less than 20 individuals in 1941 (Allen 1952) but has rebounded to over 250 individuals (Chavez-Ramirez and Wehtje 2012, Gil-Weir et al. 2012). Whooping cranes migrate approximately 4,000 km each spring and autumn, traversing much of the North American Great Plains (Lewis 1995) and periodically landing along rivers, wetlands, and other …

Relationships Among Walleye Mercury, Selenium, Stable Isotopes, Size And Age, Mark J. Fincel, Robert P. Hanten, Shannon Minerich

The Prairie Naturalist

Bioaccumulation of mercury is well-documented in aquatic ecosystems and occurs as mercury is accumulated and passed up food chains (Kidd et al. 1995, Atwell et al. 1998, Downs et al. 1998). Trophic level correlations have been widely reported for mercury (Snodgrass et al. 2000) and other metals (Barron 1995). Generally, within a system, carnivores have the highest mercury loadings, omnivores intermediate and herbivores the lowest (Phillips et al. 1980). However, little research has focused on the differences in mercury bioaccumulation for a single species that ranges across multiple trophic levels (Burger et al. 2001).

Stable isotope analysis has expanded the …

Dual Capture Of A Piñon Deer Mouse And A Silky Pocket Mouse Resulting In Consumption Of The Smaller Animal, Jon C. Pigage, Roger D. Peyton

The Prairie Naturalist

Intraspecific dual captures of rodents are uncommon, but not rare. There are several reports of brown rats (Rattus norvegicus), white-footed deer mice (Peromyscus leucopus), North American deer mice (P. maniculatus), western harvest mice (Reithrodontomys megalotis) and meadow voles (Microtus pennsylvanicus) caught simultaneously in the same trap (i.e. a door-closing live-trap; Davis 1955, Getz 1961, Blaustein and Rothstein 1978, Jenkins and Llewellyn 1981, Novak 1983). Some reports are incidental whereas others are interpreted as social associations or bonding between conspecifics. Interspecific dual captures are rarely reported in the literature. The earliest report of …

A Simple Device For Measuring The Minimum Current Velocity To Maintain Semi-Buoyant Fish Eggs In Suspension, Julias S. Mueller, Brandon D. Cheek, Qingman Chen, Jillian Groeschel, Shannok K. Brewer, Timothy B. Grabowski

The Prairie Naturalist

Pelagic broadcast spawning cyprinids are common to Great Plains rivers and streams. This reproductive guild produces non-adhesive semi-buoyant eggs that require sufficient current velocity to remain in suspension during development. Although studies have shown that there may be a minimum velocity needed to keep the eggs in suspension, this velocity has not been estimated directly nor has the influence of physicochemical factors on egg buoyancy been determined. We developed a simple, inexpensive flow chamber that allowed for evaluation of minimum current velocity needed to keep semi-buoyant eggs in suspension at any time frame during egg development. The device described here …

Associations Between Iron Concentration And Productivity In Montane Streams Of The Black Hills, South Dakota, Cari-Ann Hayer, Benjamin M. Holocmb, Steven R. Chipps

The Prairie Naturalist

Iron is an important micronutrient found in aquatic systems that can influence nutrient availability (e.g. phosphorus) and primary productivity. In streams, high iron concentrations often are associated with low pH as a result of acid mine drainage, which is known to affect fish and invertebrate communities. Streams in the Black Hills of South Dakota are generally circumneutral in pH, yet select streams exhibit high iron concentrations associated with natural iron deposits. In this study, we examined relationships among iron concentration, periphyton biomass, macroinvertebrate abundance, and fish assemblages in four Black Hills streams. The stream with the highest iron concentration (~5 …

Red-Tailed Hawk Nesting In Kansas: Influence Of Landscape Characteristics, William Langley

The Prairie Naturalist

Factors that affect nest site selection in red-tailed hawks (Buteo jamaicensis) include the physical features surrounding the nest and landscape characteristics. Features surrounding nests include: tree height, tree diameter (dbh), percent canopy cover, slope, nest openness, and density of woody plants or shrubs (Titus and Mosher 1981, Bechard et al. 1990, Tietje et al. 1997, Actkinson et al. 2007). Landscape characteristics that influence nest-site selection by red-tailed hawks vary across regions. In Georgia, red-tailed hawks selected nest site in areas with greater area of agriculture and open pasture (Moorman and Chapman 1996), whereas in Oklahoma prairies, the hawks …

Automated Conserved Non-Coding Sequence (Cns) Discovery Reveals Differences In Gene Content And Promoter Evolution Among Grasses, Gina Marie Turco, James C. Schnable, Brent S. Pedersen, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Conserved non-coding sequences (CNS) are islands of non-coding sequence that, like protein coding exons, show less divergence in sequence between related species than functionless DNA. Several CNSs have been demonstrated experimentally to function as cis-regulatory regions. However, the specific functions of most CNSs remain unknown. Previous searches for CNS in plants have either anchored on exons and only identified nearby sequences or required years of painstaking manual annotation. Here we present an open source tool that can accurately identify CNSs between any two related species with sequenced genomes, including both those immediately adjacent to exons and distal sequences separated by …

A Morphological Analysis Of A Hybrid Swarm Of Native Ulmus Rubra And Introduced U. Pumila (Ulmaceae) In Southeastern Nebraska, Christian Elowsky, Ingrid E. Jordon-Thaden, Robert B. Kaul

Nebraska Center for Biotechnology: Faculty and Staff Publications

The parental species and hybrid swarm of native Ulmus rubra Muhl. and the introduced, naturalized and weedy U. pumila L. were investigated in a 65-km transect in Lancaster, Saunders, and Butler counties in Nebraska. Thirty-two trees of U. rubra, 32 of U. pumila, and 50 of the hybrid swarm were sampled for leaves and buds and subsampled for flowers and fruits. Leaves were measured for petiole length, blade length, width, primary and secondary teeth per cm, number of secondary teeth per primary tooth, and texture. Buds were scored for color and distribution of trichomes. Flowers were sampled for stamen counts …

Rate Of Shattercane × Sorghum Hybridization In Situ, Jared J. Schmidt, Jeffrey F. Pedersen, Mark L. Bernards, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Cultivated sorghum [Sorghum bicolor (L.) Moench subsp. bicolor] can interbreed with a feral weedy relative shattercane [S. bicolor nothosubsp. drummondii (Steud.) de Wet ex Davidse]. Traits introduced from cultivated sorghum could contribute to the invasiveness of a shattercane population. An experiment was conducted to determine the potential for pollenmediated gene flow from grain sorghum to shattercane. Shattercane with juicy midrib (dd) was planted in soybean [Glycine max (L.) Merr.] fields during 2 yr in concentric arcs at varying distances from a 0.39 ha sorghum pollen source with dry midrib (DD). The arcs …

2013 Fall Seed Guide, Teshome Regassa, P. Stephen Baenziger, Greg Kruger, Dipak Santra

Department of Agronomy and Horticulture: Faculty Publications

Nebraska Winter Wheat Variety Tests

Locations for Winter Wheat

Winter Wheat Characteristics

Alfalfa Variety Tests

Cool Season Grasses

Winter Barley Variety Tests

Triticale

NCIA Seed Book

A Floristic Analysis And Comparison Of Plant Communities In Harlan County, Nebraska, Naomi D. Hastings, Steven J. Rothenberger

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Historically, the Republican River Valley in Harlan County, Nebraska, has been extensively explored, but the county flora has continually been under-represented and has been found to lack records for plants that are considered common. During the 2009 growing season, a taxonomic study and comparison of 3 different sites in Harlan County was made. Site #1 was pastureland and Sites #2 and #3 were both riparian, located on the north side of the Republican River. Collections were pressed, dried, identified, and deposited in the University of Nebraska at Kearney Herbarium (NEBK). Forty-eight new county records were verified, in­cluding cutleaf-cucumber (Cyclanthera …

Distribution Of Burrowing Owls In East- Central South Dakota, Jiill A. Shaffer, Jason P. Thiele

The Prairie Naturalist

Western burrowing owl (Athene cunicularia hypugaea) populations have declined across much of western North America, particularly at the northern and eastern edges of the species’ breeding range (Martell et al. 2001, Murphy et al. 2001, Shyry et al. 2001, Skeel et al. 2001, Klute et al. 2003). In South Dakota, the burrowing owl is a summer resident that historically was relatively common throughout the state, but its range has decreased in recent decades, especially in the eastern half of the state (Whitney et al. 1978, South Dakota Ornithologists’ Union [SDOU] 1991, Peterson 1995). Tallman et al. (2002) described the species …

Notes: Nesting Patterns Of Red-Tailed Hawks And Great-Horned Owls In South-Cen- Tral Kansas, William Langley

The Prairie Naturalist

Red-tailed hawks (Buteo jamaicensis) and great-horned owls (Bubo virginianus) can influence the nesting behavior of each other through direct competition for nesting sites. For instance, owls begin nesting before hawks and, thus, can use nests from the previous year and prevent hawks from occupying those sites (Orians and Kuhl- man 1956, Gilmer et al. 1983, Minor et al. 1993). However, both species sometimes kill the nestlings of the other (Craig- head and Craighead 1956, Bosakowski et al. 1989).

The objective of my study was to investigate nesting patterns of these two raptors at the edge of the tallgrass prairie over …

Research Notes Vs. Research Articles, Christopher N. Jacques

The Prairie Naturalist

Greetings GPNSS members! I hope this email finds you well, winding down another academic year, and looking forward to the summer field season. Warm temperatures, peak foliage, and heightened anticipation of the start of another field season are upon us following a long winter season and unusually wet spring for many of us across the Great Plains. Here in westcentral Illinois, wild turkeys are gobbling, neotropical migrants are singing and establishing breeding territories, and white-tailed deer are within days of giving birth to the next generation of fawns. Granted, there are many outdoor activities to participate in, though oppressive heat …

Contributions Of Stocked And Naturally Reproduced Rainbow Trout In The Deerfield Reservoir System, Jacob L. Davis, Jerry W. Wilhite, Greg Simpson, Michael E. Barnes, Kaite N. Bertrand, David W. Willis

The Prairie Naturalist

Deerfield Reservoir in the Black Hills of South Dakota and its tributary system are managed as hatchery supplemented rainbow trout (Oncorhynchus mykiss) fisheries. Three genetically unique strains of rainbow trout (Shasta, Erwin and McConaughy) are stocked into the system. Recently, juvenile rainbow trout of unknown origin were collected in the tributary system above Deerfield Reservoir, indicating potential natural reproduction. Understanding the genetic origins and ability of these rainbow trout to contribute to the fishery is essential in determining the proper management strategy for these waters. Our objectives were to 1) evaluate the genetic origins of potentially naturally reproduced rainbow trout …

Taxonomy And Biogeography Of The New Caledonian Species Of Xylopia L. (Annonaceae), David M. Johnson, Jérôme Munzinger, Julie A. Peterson, Nancy A. Murray

Department of Entomology: Faculty Publications

A taxonomic review of the New Caledonian representatives of the pantropical tree genus Xylopia L. (Annonaceae) is presented based on museum study of 157 collections. Distinctions are clarified among the four endemic species, two of them previously undescribed in flower and two others described in the literature from only the type collections. A key and species descriptions are presented. Xylopia pancheri Baill., the most frequently collected species, occurs in maquis environments with ultramafic rocks in the central and southern regions of the island, as well as on the Ile des Pins. Xylopia vieillardii Baill. is widespread in low elevation humid …

Oaks For Nebraska, Justin R. Evertson

Nebraska Statewide Arboretum Publications

R = belongs to red oak group—acorns mature over two seasons & leaves typically have pointed lobes.
W = belongs to white oak group— acorns mature in one season & leaves typically have rounded lobes.

Estimated size range is height x spread for trees growing in eastern Nebraska.

A few places to see oaks: Indian Cave State Park; Krumme Arboretum in Falls City; Peru State College; Fontenelle Nature Center in Bellevue; Elmwood Park in Omaha; Wayne Park in Waverly; University of Nebraska Lincoln; Lincoln Regional Center Arboretum; Ehman Park in Gothenburg; Highland Park in Hastings; UNL Extension Center & Cody …

Genetic Mapping And Confirmation Of Quantitative Trait Loci For Seed Protein And Oil Contents And Seed Weight In Soybean, Safiullah M. Pathan, Tri Vuong, Kerry Clark, Jeong-Dong Lee, J. Grover Shannon, Craig A. Roberts, Mark R. Ellersieck, Joseph W. Burton, P. B. Cregan, David L. Hyten, Henry T. Nguyen, David A. Sleper

Department of Agronomy and Horticulture: Faculty Publications

Demand for soybean [Glycine max (L.) Merr.] meal has increased worldwide and soybean importers often offer premiums for soybean containing higher contents of protein and oil. Objectives were to detect quantitative trait loci (QTL) associated with soybean seed protein, oil, and seed weight in a soybean mapping population and confirm detected QTL across genetic backgrounds and environments. Two populations of 216 and 156 recombinant inbred lines were developed from Magellan × PI 438489B and Magellan × PI 567516C crosses, respectively, and grown in two Missouri environments in 2008. More than 900 simple sequence repeat (SSR) and single nucleotide polymorphism …

Resource Allocation For Maximizing Prediction Accuracy And Genetic Gain Of Genomic Selection In Plant Breeding: A Simulation Experiment, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Allocating resources between population size and replication affects both genetic gain through phenotypic selection and quantitative trait loci detection power and effect estimation accuracy for marker-assisted selection (MAS). It is well known that because alleles are replicated across individuals in quantitative trait loci mapping and MAS, more resources should be allocated to increasing population size compared with phenotypic selection. Genomic selection is a form of MAS using all marker information simultaneously to predict individual genetic values for complex traits and has widely been found superior to MAS. No studies have explicitly investigated how resource allocation decisions affect success of genomic …

Evaluation Of Public Sweet Sorghum A-Lines For Use In Hybrid Production, Jeffrey F. Pedersen, Scott E. Sattler, William F. Anderson

Department of Agronomy and Horticulture: Faculty Publications

A fundamental need for commercialization of sweet sorghum [Sorghum bicolor (L.) Moench] as a bioenergy crop is an adequate seed supply, which will require development of hybrid varieties using dwarf seed-parent lines. A set of six public sweet sorghum A-lines (Dwarf Kansas Sourless, KS9, N36, N38, N39, and N4692) were crossed with a set of six public sweet sorghum cultivars (Brawley, Kansas Collier, Dale, Sugar Drip, Waconia, and Wray). Grain, fiber, and sugar yields were determined, and conversion formulas were applied to estimate ethanol yields. Hybrids were grown in fields at Ithaca, NE, USA, in 1983– 1984 fertilized with …

The Clubroot Pathogen (Plasmodiophora Brassicae) Influences Auxin Signaling To Regulate Auxin Homeostasis In Arabidopsis, Linda Jahn, Stefanie Mucha, Sabine Bergmann, Cornelia Horn, Paul E. Staswick, Bianka Steffens, Johannes Siemens, Jutta Ludwig-Müller

Department of Agronomy and Horticulture: Faculty Publications

The clubroot disease, caused by the obligate biotrophic protist Plasmodiophora brassicae, affects cruciferous crops worldwide. It is characterized by root swellings as symptoms, which are dependent on the alteration of auxin and cytokinin metabolism. Here, we describe that two different classes of auxin receptors, the TIR family and the auxin binding protein 1 (ABP1) in Arabidopsis thaliana are transcriptionally upregulated upon gall formation. Mutations in the TIR family resulted in more susceptible reactions to the root pathogen. As target genes for the different pathways we have investigated the transcriptional regulation of selected transcriptional repressors (Aux/IAA) and transcription …

Sub1a-Mediated Submergence Tolerance Response In Rice Involves Differential Regulation Of The Brassinosteroid Pathway, Aaron J. Schmitz, Jing J. Folsom, Yusuke Jikamaru, Pamela C. Ronald, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Submergence 1A (SUB1A), is an ethylene response factor (ERF) that confers submergence tolerance in rice (Oryza sativa) via limiting shoot elongation during the inundation period. SUB1A has been proposed to restrict shoot growth by modulating gibberellic acid (GA) signaling.

Our transcriptome analysis indicated that SUB1A differentially regulates genes associated with brassinosteroid (BR) synthesis during submergence. Consistent with the gene expression data, the SUB1A genotype had higher brassinosteroid levels after submergence compared to the intolerant genotype. Tolerance to submergence can be activated in the intolerant genotype by pretreatment with exogenous brassinolide, which results in restricted shoot elongation …

Unleashing The Potential Of The Root Hair Cell As A Single Plant Cell Type Model In Root Systems Biology, Zhenzhen Qiao, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Plant root is an organ composed of multiple cell types with different functions.This multicellular complexity limits our understanding of root biology because -omics studies performed at the level of the entire root reflect the average responses of all cells composing the organ. To overcome this difficulty and allow a more comprehensive understanding of root cell biology, an approach is needed that would focus on one single cell type in the plant root. Because of its biological functions (i.e., uptake of water and various nutrients; primary site of infection by nitrogen-fixing bacteria in legumes), the root hair cell is an attractive …