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Low-Copy Nuclear Gene And Mcgish Resolves Polyploid History Of Eleusine Coracana And Morphological Character Evolution In Eleusine, Qing Liu, Bin Jiang, Jun Wen, Paul Michael Peterson Jan 2014

Low-Copy Nuclear Gene And Mcgish Resolves Polyploid History Of Eleusine Coracana And Morphological Character Evolution In Eleusine, Qing Liu, Bin Jiang, Jun Wen, Paul Michael Peterson

Turkish Journal of Botany

Eleusine coracana (L.) Gaertn. (finger millet) is the third most important cereal crop in semiarid regions of the world, but the degree of relatedness of finger millet with other species in the genus is unverified. The study of morphological character evolution in Eleusine Gaertn. has lagged behind due to lack of relevant research. Polyploidy history of finger millet was analyzed using waxy sequences together with multicolor genomic in situ hybridization (McGISH). In the waxy phylogenetic tree, sequences of 2 homoeologous loci were isolated from 3 tetraploids: Eleusine coracana, E. africana Kenn.-O'Byrne, and E. kigeziensis S.M.Phillips. The 3 species grouped with …


A Revision Of Centaurea Sect. Centaurea (Asteraceae) From Iran, Massoud Ranjbar, Kazem Negaresh Jan 2014

A Revision Of Centaurea Sect. Centaurea (Asteraceae) From Iran, Massoud Ranjbar, Kazem Negaresh

Turkish Journal of Botany

A taxonomic review of Centaurea L. sect. Centaurea (Asteraceae) in Iran was carried out. Seven taxa are recognized and 1 of them is described as a new species: Centaurea joharchii Ranjbar & Negaresh. This new species has been described and illustrated from Khorasan Province, SE Iran. A new combination is proposed: C. kermanshahensis (Wagenitz) Ranjbar & Negaresh. In addition, C. aggregata Fisch. & C.A.Mey. ex DC. subsp. aggregata is typified and C. diffusa Lam. is reported here as a new record for Iran. Finally, a key to all species of the section Centaurea in Iran is provided.


Does Gypsum Influence Seed Germination?, Eva M. Cañadas, Miguel Ballesteros, Francisco Valle, Juan Lorite Jan 2014

Does Gypsum Influence Seed Germination?, Eva M. Cañadas, Miguel Ballesteros, Francisco Valle, Juan Lorite

Turkish Journal of Botany

Flora inhabiting gypsum outcrops in arid environments shows a high level of specialization. However, the processes involved are still unclear, specifically at the key stage of germination. Here, to assess whether gypsum could chemically influence seed germination, we tested the germination of species according to 3 functional groups: gypsophiles, gypsovags, and calcicoles. A total of 24 taxa were selected, all occurring in gypsum and limestone substrates, under a semiarid and dry Mediterranean climate. Three levels of gypsum (CaSO_{4}^.2H_{2}O) solution (low = 0.5 g/L, medium = 1 g/L, and high = 2.4 g/L) and 1 control treatment of distilled water were …


Micropropagation Of Erodium Sibthorpianum Subsp. Sibthorpianum, An Endemic Threatened Species Of Uludağ Mountain (Bursa-Turkey), Betül Akin, İsmai̇l Kocaçalişkan, Gürcan Güleryüz Jan 2014

Micropropagation Of Erodium Sibthorpianum Subsp. Sibthorpianum, An Endemic Threatened Species Of Uludağ Mountain (Bursa-Turkey), Betül Akin, İsmai̇l Kocaçalişkan, Gürcan Güleryüz

Turkish Journal of Botany

Uludağ Mountain is one of the Important Plant Areas of Turkey. Its native plant species are threatened by various human and environmental factors. In this study, 2 different micropropagation techniques, with Murashige Skoog (MS) and modified MS (ModMS) media, were used for Erodium sibthorpianum Boiss. subsp. sibthorpianum. Mineral composition was developed according to the plant requirements. Shoot tips were cultured on these media. Seedlings, obtained from in vitro germinated seeds, were used as explant sources in micropropagation studies. The highest percentage of shoot induction (11.33 shoots/explant) was determined in 1 ppm 6-benzyl amino purine and plant growth regulator-free MS medium …


A New Species Of Onobrychis Sect. Onobrychis (Fabaceae) From Iran, Atefe Amirahmadi, Shahrokh Kazempour Osaloo, Maryam Khoshsokhan Mozaffar, Mohammad Mehdi Charkhchian Jan 2014

A New Species Of Onobrychis Sect. Onobrychis (Fabaceae) From Iran, Atefe Amirahmadi, Shahrokh Kazempour Osaloo, Maryam Khoshsokhan Mozaffar, Mohammad Mehdi Charkhchian

Turkish Journal of Botany

Onobrychis alamutensis Amirah., Kaz. Osaloo & Charkhch. is described as a new species. It is restricted to the northwestern Alborz mountain range in Rudbar-Alamut, Qazvin, Iran. The diagnostic characters and relevant description of the species are given. The new species is distinguished from its closely related taxa, Onobrychis verae Sirj., O. ptychophylla Sirj. & Rech.f., and O. sosnowskyi Grossh. in both morphological and molecular evidence (nrDNA ITS sequences). The distribution map and an illustration of the new species are also provided.


Census, Reproductive Biology, And Germination Of Astragalus Gines-Lopezii (Fabaceae), A Narrow And Endangered Endemic Species Of Sw Spain, Vanesa Martínez-Fernández, Felipe Martinez-Garcia, Felix Perez Garcia Jan 2014

Census, Reproductive Biology, And Germination Of Astragalus Gines-Lopezii (Fabaceae), A Narrow And Endangered Endemic Species Of Sw Spain, Vanesa Martínez-Fernández, Felipe Martinez-Garcia, Felix Perez Garcia

Turkish Journal of Botany

Astragalus gines-lopezii Talavera, Podlech, Devesa & F.M.Vázquez (Fabaceae) is a threatened endemic species with a distribution restricted to a very small area in Badajoz Province (Extremadura Region, SW Spain) and only 2 populations are known. This species was catalogued in the \"Endangered\" category in the 2008 Red List and the 2010 Threatened Spanish Vascular Flora List. Despite its status as an endangered species, at present very little is known about the distribution, census, and reproductive biology of this species. In this study we have carried out an exhaustive census of A. gines-lopezii, and we have evaluated the production of flowers, …


Patterns Of Phylogeography And Vicariance Of Chamaerops Humilis L. (Palmae), Juan Luis García-Castaño, Anass Terrab, María Ángeles Ortiz, Tod Falor Stuessy, Salvador Talavera Jan 2014

Patterns Of Phylogeography And Vicariance Of Chamaerops Humilis L. (Palmae), Juan Luis García-Castaño, Anass Terrab, María Ángeles Ortiz, Tod Falor Stuessy, Salvador Talavera

Turkish Journal of Botany

Chamaerops humilis L. is 1 of 2 native palms occurring in Europe and the only native palm in the West Mediterranean region. Our aims were: (1) to describe its phylogeographic structure; (2) to infer a biogeographic scenario to explain its present distribution; and (3) to assess changes in its distribution from the last interglacial period. Twenty-two populations were sampled. An amplified fragment length polymorphism analysis produced 226 fragments, which allowed recognition of 4 groups of populations: (1) E Iberian Peninsula plus Al Hoceima (NE Morocco), the Balearics, and Sardinia; (2) France, the Italian Peninsula, and Sicily; (3) SW Iberian Peninsula …


Genome Size And Phylogenetic Relationships Between The Tunisian Species Of The Genus Calligonum (Polygonaceae), Hassen Gouja, Alfredo Garcia Fernández, Teresa Garnatje, Aly Raies, Mohamed Neffati Jan 2014

Genome Size And Phylogenetic Relationships Between The Tunisian Species Of The Genus Calligonum (Polygonaceae), Hassen Gouja, Alfredo Garcia Fernández, Teresa Garnatje, Aly Raies, Mohamed Neffati

Turkish Journal of Botany

The species of the genus Calligonum L. (Polygonaceae) that appear in Tunisian deserts play an important role in maintaining local ecosystems and supply important natural resources in these regions. In this study, 31 individuals belonging to the 3 hypothetical species of the genus Calligonum (Calligonum arich Le Houér., Calligonum azel Maire, and Calligonum comosum L'Hér.) were collected from 12 populations in 6 localities of the Tunisian desert and examined to assess the relationship between species. Phylogenetic analyses using 1 nuclear (ITS) and 2 plastid regions (trnL-trnF and rbcL) and genome size assessments are used in this study to evaluate the …


Gladiolus Osmaniyensis (Iridaceae), A New Species From South Anatolia, Turkey, Mehmet Sağiroğlu, Gençay Akgül Jan 2014

Gladiolus Osmaniyensis (Iridaceae), A New Species From South Anatolia, Turkey, Mehmet Sağiroğlu, Gençay Akgül

Turkish Journal of Botany

A new species, Gladiolus osmaniyensis Sağıroğlu (Iridaceae), is described and illustrated from South Anatolia, Turkey. G. osmaniyensis is morphologically close to G. attilae and G. atroviolaceus. The ecology and phenology of the new species as well as its etymology, conservation status, and diagnostic morphological features are discussed. In addition, the seed surfaces of the G. osmaniyensis, G. attilae, and G. atroviolaceus are examined by SEM. The geographical distribution of the new species and the morphologically related species are mapped as well.


Modulation Of Osmotic Adjustment And Enzymatic Antioxidant Profiling In Apera Intermedia Exposed To Salt Stress, Evren Yildiztugay, Ceyda Özfi̇dan Konakçi, Mustafa Küçüködük, Yağmur Duran Jan 2014

Modulation Of Osmotic Adjustment And Enzymatic Antioxidant Profiling In Apera Intermedia Exposed To Salt Stress, Evren Yildiztugay, Ceyda Özfi̇dan Konakçi, Mustafa Küçüködük, Yağmur Duran

Turkish Journal of Botany

The effects of salinity on growth, osmotic adjustment, and antioxidative responses were evaluated in Apera intermedia. For this purpose, 30-day-old plants were irrigated every other day with Hoagland nutrient solution containing 0, 150, 300, or 600 mM NaCl for 7 and 14 days. The results showed inhibition of growth, relative growth rate, relative water content, and osmotic potential with increasing NaCl concentration. Increased Na^+, Cl^-, and Na^{+}/K^+ ratio and decreased K^+ and Ca^{2+} were determined with increasing NaCl concentrations. The activities of superoxide dismutase and ascorbate peroxidase were conspicuously enhanced at 150 mM NaCl, but activities of catalase, peroxidase, and …


Salicylic-Acid-Induced Recovery Ability In Salt-Stressed Hordeum Vulgare Plants, Hadi Pirasteh Anosheh, Gholamhassan Ranjbar, Yahya Emam, Muhammad Ashraf Jan 2014

Salicylic-Acid-Induced Recovery Ability In Salt-Stressed Hordeum Vulgare Plants, Hadi Pirasteh Anosheh, Gholamhassan Ranjbar, Yahya Emam, Muhammad Ashraf

Turkish Journal of Botany

The recovery ability of barley plants from salt stress conditions was assessed using foliar application of salicylic acid (SA) in a study conducted under controlled conditions. The barley plants (Hordeum vulgare L. 'Reyhan') were subjected to saline water with varying salinity levels (tap water 0.67 dS m^{-1} as control, 3, 6, 9, and 12 dS m^{-1}) from 14 to 42 days after sowing (DAS). Then the plants were subjected to recovery treatments for 4 weeks, from 42 to 70 DAS. The recovery treatments included: non-recovery (R_0), irrigation with tap water (R_1), and irrigation with tap water + 2 foliar applications …


Protein Mobilisation And Proteolytic Activities In Root Tubers Of Asphodelus Aestivus, Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan Jan 2014

Protein Mobilisation And Proteolytic Activities In Root Tubers Of Asphodelus Aestivus, Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan

Turkish Journal of Botany

We studied protein mobilisation in Asphodelus aestivus Brot. (Liliaceae) during a 1-year period. The activities of 3 proteolytic enzymes, endopeptidase (EP), leucine aminopeptidase (LAP), and carboxypeptidase (CP), together with soluble protein levels and total nitrogen and water content in root tubers were investigated. We found that protein mobilisation continued throughout the year. The highest levels of the 3 proteolytic enzymes were found in different months: LAP in December (1.19 ± 0.19 µmol pNA/g FW), CP in February (14.19 ± 1.13 µmol Leu/g FW), and EP in May (5.18 ± 0.12 U/h). Activities of LAP and CP generally reached their higher …


New Records Of Discomycetous Fungi From Ukraine, Irina Ihorivna Morozova Jan 2014

New Records Of Discomycetous Fungi From Ukraine, Irina Ihorivna Morozova

Turkish Journal of Botany

Eight species of discomycetous fungi found in the forests of the Kharkiv region for the first time in Ukraine are described: Dermea acerina (Peck) Rehm, Chlorencoelia versiformis (Pers.) J.R.Dixon, Arachnopeziza aurata Fuckel, Arachnopeziza obtusipila Grélet, Hyaloscypha intacta Svrcek, Lachnum brevipilosum Baral, Pezizella vulgaris (Fr.) Sacc., and Gyromitra fastigiata (Krombh.) Rehm. Original illustrations, descriptions, and data on the distribution of these species in the world are given.


New Lauraceous Species From The Siwalik Forest Of Arunachal Pradesh, Eastern Himalaya, And Their Palaeoclimatic And Palaeogeographic Implications, Mahasin Ali Khan, Subir Bera Jan 2014

New Lauraceous Species From The Siwalik Forest Of Arunachal Pradesh, Eastern Himalaya, And Their Palaeoclimatic And Palaeogeographic Implications, Mahasin Ali Khan, Subir Bera

Turkish Journal of Botany

Leaf remains of 4 lauraceous taxa collected from the Siwalik sediments (Middle Miocene to Pliocene) of Arunachal Pradesh of eastern Himalaya are described in the present communication. One fossil leaf, Cinnamomum palaeobejolghota sp. nov., was recovered from the lower part of the Siwalik succession (Dafla Formation; Middle to Upper Miocene), while Persea mioparviflora sp. nov., Persea preglaucescens sp. nov., and Lindera neobifaria sp. nov. were collected from the middle part of the Siwalik succession (Subansiri Formation; Pliocene) of Arunachal sub-Himalaya. The geographic distribution of the fossils and their modern counterparts is discussed and on that basis tropical vegetation under warm …


Studies On New Fungal Records For Turkish Mycota From Trabzon, Ertuğrul Sesli̇ Jan 2014

Studies On New Fungal Records For Turkish Mycota From Trabzon, Ertuğrul Sesli̇

Turkish Journal of Botany

Fungal specimens were collected and photographed between 2010 and 2013 around Trabzon. Ecological observations were made in the field, and the structures of pileipellis, basidia, spores, marginal cells, and hyphae were illuminated in the laboratory. Some dried specimens were compared with British collections in the Royal Botanic Gardens, Kew. Finally, Cortinarius parevernius Rob. Henry, Cortinarius tortuosus (Fr.) Fr., Entoloma euchroum (Pers.) Donk, Entoloma lividoalbum (Kühner & Romagn.) Kubicka, Hygrophoropsis morganii (Peck) H.E.Bigelow, Phaeocollybia festiva (Fr.) R.Heim, and Tricholoma viridilutescens M.M.Moser were recorded for the first time for the Turkish Mycota. The new records were illustrated, briefly described, and discussed.


Orthotrichum Pamiricum (Bryophyta), A New Epiphytic Moss Species From Pamir Mountains In Central Asia, Vitezslav Plasek, Jakub Sawicki, Lukas Cihal Jan 2014

Orthotrichum Pamiricum (Bryophyta), A New Epiphytic Moss Species From Pamir Mountains In Central Asia, Vitezslav Plasek, Jakub Sawicki, Lukas Cihal

Turkish Journal of Botany

Orthotrichum pamiricum Plasek & Sawicki is described as a new species from the western foothills of the Pamir Mountains of the Tajik-Afghan border in Central Asia. It is placed in Orthotrichum Hedw. subg. Pulchella (Schimp.) Vitt. The species is characterized by obtuse leaf apices, cryptoporous stomata, an exostome composed of 8 pairs of the teeth that are recurved when dry, and an endostome with 16 thin and incurved segments. Molecular data indicating the phylogenetic placement of O. pamiricum are also presented.


Planktothrix Rubescens: A Perennial Presence And Toxicity In Lake Sapanca, Reyhan Akçaalan Albay, Lati̇fe Köker, Cenk Gürevi̇n, Meri̇ç Albay Jan 2014

Planktothrix Rubescens: A Perennial Presence And Toxicity In Lake Sapanca, Reyhan Akçaalan Albay, Lati̇fe Köker, Cenk Gürevi̇n, Meri̇ç Albay

Turkish Journal of Botany

Cyanobacteria are expected to adjust to new conditions better than other primary producers since they have a long history of adaptation as one of the first organisms on earth. Their ecophysiological adaptations differ widely among cyanobacterial species. As a stratified species, P. rubescens forms a population in the metalimnion during summer months in lakes with clear water and disperses in the whole water column during the period. However, after a dry season in 2006 and 2007, a prolonged surface bloom in late winter was observed in Lake Sapanca The maximum biomass of P. rubescens reached 13.9 mg L^{-1} in surface …


Molecular Variability Of Plantain Ecotypes From The Genus Musa (Musaceae), Rakeshkumar Choudhary, Raghunath Keshavachandran, Rema Menon, Ganesh Khalekar, Nirbhay Singh, Divya Maruthiyottu Jan 2014

Molecular Variability Of Plantain Ecotypes From The Genus Musa (Musaceae), Rakeshkumar Choudhary, Raghunath Keshavachandran, Rema Menon, Ganesh Khalekar, Nirbhay Singh, Divya Maruthiyottu

Turkish Journal of Botany

Twelve plantain ecotypes were characterized for molecular variability using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of the 60 RAPD and 40 ISSR primers tested, 16 RAPD and 14 ISSR primers were found polymorphic, and were used for molecular profiling of the plantain ecotypes. The average number of bands per primer, average number of polymorphic bands per primer, mean polymorphic information content (PIC) value, resolving power (Rp), and marker indices (MI) for RAPD primers were 8.63, 3.25, 0.87, 15.49, and 2.86, whereas the respective values for the ISSR assay were 7.93, 4.14, 0.85, 12.49, …


The Assessment Of Genetic Diversity Of Castanea Species By Rapd, Aflp, Issr, And Ssr Markers, Sofiane Abdelhamid, Cong-Linh Lê, Marco Conedera, Philippe Küpfer Jan 2014

The Assessment Of Genetic Diversity Of Castanea Species By Rapd, Aflp, Issr, And Ssr Markers, Sofiane Abdelhamid, Cong-Linh Lê, Marco Conedera, Philippe Küpfer

Turkish Journal of Botany

Castanea Mill. (chestnut) is a multipurpose deciduous tree and member of the family Fagaceae, widely distributed throughout North America, Europe, and Asia. The evaluation of the genetic diversity of chestnut species is crucial for the effective conservation of this economically and ecologically valuable tree. In this study, we applied 4 DNA markers to detect the genetic variability among and within Castanea species and to compare the effectiveness of each system in estimating genetic variation. We amplified 106 random amplified polymorphic DNA (RAPD), 228 amplified fragment length polymorphism (AFLP), 42 intersimple sequence repeat (ISSR), and 36 simple sequence repeat (SSR) polymorphic …


Reseda Malatyana (Resedaceae), A New Chasmophytic Species From Eastern Anatolia, Turkey, Hasan Yildirim, Serdar Gökhan Şenol Jan 2014

Reseda Malatyana (Resedaceae), A New Chasmophytic Species From Eastern Anatolia, Turkey, Hasan Yildirim, Serdar Gökhan Şenol

Turkish Journal of Botany

Reseda malatyana Yıldırım & Şenol (Resedaceae) is illustrated and described as a new species to science, endemic to eastern Anatolia, Turkey. Reseda malatyana is related to Reseda armena Boiss. It differs from Reseda armena by the following characters: densely smaller, unbranched at the upper part, papillate-hispidulous stems; dark green, thicker, and densely papillate-hispidulous leaves; bigger petals; smaller pedicels; relatively smaller with sinus seeds; oblate-spheroidal, tricolporate, and rugulate pollen grains. R. malatyana is a perennial species that colonizes on marlstone rocky cliffs, known from a single locality in Levent Canyon, located in Malatya Province. It is an obligated chasmophytic species. In …


The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü Jan 2014

The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü

Turkish Journal of Botany

Watermelon fruit is very sensitive to ethylene, showing rapid deterioration and water-soaking upon ethylene exposure. The objective of this study was to isolate and determine the expressions of ethylene receptor genes in watermelon fruit. As a result of PCR amplification of cDNAs obtained via RT-PCR of total RNA from watermelon fruit with degenerate primers, 3 partial cDNAs were obtained. After extension of the 3' and 5' ends of the cDNAs via 3' and 5' RACE analysis, 3 full-length genes designated as CL-ETR1, CL-ERS1, and CL-ETR2 were obtained. Upon cloning and sequencing, it was determined that the CL-ETR1, CL-ERS1, and CL-ETR2 …


Nonsense-Mediated Decay Of Sucrose Synthase 1 Mrna With Induced Premature Chain Termination Codon During Cold Acclimation In Winter Wheat, Rita Armoniene, Gintaras Brazauskas Jan 2014

Nonsense-Mediated Decay Of Sucrose Synthase 1 Mrna With Induced Premature Chain Termination Codon During Cold Acclimation In Winter Wheat, Rita Armoniene, Gintaras Brazauskas

Turkish Journal of Botany

Cold acclimation induces the expression of cold-regulated genes needed to protect plants against freezing damage. Fourteen candidate genes were identified as being differentially expressed (presence/absence) between cold acclimated and nonacclimated crown and leaf tissues of 2 winter wheat lines using the cDNA-AFLP method. Two TILLING populations originating from the 2 winter wheat lines were further developed in order to create mutant forms of the candidate genes to verify their role in freezing-tolerance formation. Exon 8 of the identified differentially expressed sucrose synthase 1 (Ss1) gene was chosen for mutation detection by high-resolution melting analysis. A total of 75.68 kb of …


First Karyotype Analysis, Physical Rdna Mapping, And Genome Size Assessment In 4 North African Astragalus Taxa (Fabaceae), Karim Baziz, Meriem Benamara-Bellagha, Fatima Pustahija, Spencer Craig Brown, Sonja Siljak-Yakovlev, Nadra Khalfallah Jan 2014

First Karyotype Analysis, Physical Rdna Mapping, And Genome Size Assessment In 4 North African Astragalus Taxa (Fabaceae), Karim Baziz, Meriem Benamara-Bellagha, Fatima Pustahija, Spencer Craig Brown, Sonja Siljak-Yakovlev, Nadra Khalfallah

Turkish Journal of Botany

Four taxa of Algerian Astragalus L. were studied for their chromosome number, karyotype features, and genome size. Fluorochrome banding was done for detection of GC-rich DNA regions, fluorescence in situ hybridization (FISH) for physical mapping of 35S and 5S rRNA genes, and flow cytometry for nuclear DNA content. All the taxa present the same chromosome number (2n = 2x = 16) and single 35S and 5S rDNA loci, but their distributions on chromosomes are different. The GC heterochromatin pattern was different among studied taxa and an unusually high number of chromomycin-positive bands were observed in A. pseudotrigonus Batt. & Trab. …


A Taxonomic Reassessment Of The Tanacetum Aureum (Asteraceae, Anthemideae) Species Group: Insights From Morphological And Molecular Data, Marzie Kazemi, Ali Sonboli, Hassan Zare Maivan, Shahrokh Kazempour Osaloo Jan 2014

A Taxonomic Reassessment Of The Tanacetum Aureum (Asteraceae, Anthemideae) Species Group: Insights From Morphological And Molecular Data, Marzie Kazemi, Ali Sonboli, Hassan Zare Maivan, Shahrokh Kazempour Osaloo

Turkish Journal of Botany

A taxonomic reassessment of the Tanacetum aureum (Lam.) Greuter, M.V.Agab. & Wagenitz (Asteraceae, Anthemideae) species group was performed. This group of species is distinguished by herbaceous perennial habit, (1-)2-10(-80) disciform capitula, with compact to lax corymbs and strongly to moderately convex receptacle. A multivariate statistical analysis of 58 morphological characters using Ward cluster, principal component analyses, and molecular phylogenetic analyses of nrDNA ITS and cpDNA rpL32-trnL^{(UAG)} + ndhF-rpL32 spacer sequence datasets were conducted. Both datasets corroborated that T. aureum, T. oligocephalum (DC.) Sch.Bip., and T. heimerlii (Nabelek) Parsa could be differentiated as separate species. An identification key of the studied …


Toxicological Evaluation Of Polar And Nonpolar Components Of Isodon Coetsa (Lamiaceae), Sandhya Vincent Neelamkavil, John Ernest Thoppil Jan 2014

Toxicological Evaluation Of Polar And Nonpolar Components Of Isodon Coetsa (Lamiaceae), Sandhya Vincent Neelamkavil, John Ernest Thoppil

Turkish Journal of Botany

Isodon coetsa (Buch.-Ham. ex D.Don) Kudo, a perennial herb used in traditional Chinese folk medicines as an antibacterial, antiinflammatory, and antitumour agent, was subjected to toxicity analysis. Cytotoxic screening using the Allium L. assay was done with extracts containing polar and nonpolar compounds as well as with polar compounds alone. Root tips were treated with different concentrations of both the extracts, 0.005%, 0.01%, 0.05%, and 0.1%, for varying time durations (30 min, 1 h, 2 h, and 3 h). Extremely significant levels of chromosome abnormality (P < 0.001) were observed with the extracts when compared with the positive control (0.01% methyl parathion), but the extract with polar and nonpolar compounds showed higher abnormality percentage. Not much variation was observed in the mitotic index when compared to the negative control (distilled water). Studies revealed many clastogenic and nonclastogenic abnormalities. The major abnormalities included chromosome fragments, stickiness, ring chromosomes, chromosome bridges, pulverization, binucleate cell, micronucleus, ball metaphase, chromosome laggards, and shift in microtubule organizing center. The results showed that the nonpolar components possessed more clastogenic activity, which can be specifically targeted in order to destroy cancer cells. The toxic activity of extracts also suggests the need of judicious use of I. coetsa in folk medicines.


Contrasting Inbreeding Depression In Early And Late Stages Of The Life Cycle Of A Mediterranean Shrub, Anagyris Foetida (Leguminosae), Francisco Javier Valtueña, Josefa López, Ana Ortega-Olivencia, Tomás Rodriguez-Riaño, Miguel González Jan 2014

Contrasting Inbreeding Depression In Early And Late Stages Of The Life Cycle Of A Mediterranean Shrub, Anagyris Foetida (Leguminosae), Francisco Javier Valtueña, Josefa López, Ana Ortega-Olivencia, Tomás Rodriguez-Riaño, Miguel González

Turkish Journal of Botany

Anagyris foetida L. has a Mediterranean distribution, but its populations are highly fragmented. This, together with its mixed mating system, puts it at clear risk of being strongly affected by inbreeding depression. To determine the effect of inbreeding depression in this species, hand pollinations were performed during the 2003/04 season in 2 populations from SW Spain to generate selfed and outcrossed seeds. These seeds were divided in 2 sample lots and seedlings were obtained in different years. During the first year of life the first lot showed no differences in vegetative parameters between the 2 populations, so that no differentiation …


Genetic Stability In A Predominating Turkish Olive Cultivar, Gemlik, Assessed By Rapd, Microsatellite, And Aflp Marker Systems, Ufuk Çeli̇kkol Akçay, Gülcan Özkan, Beki̇r Şan, Oğuz Dolgun, Ayhan Dağdelen, Di̇lşat Bozdoğan Konuşkan Jan 2014

Genetic Stability In A Predominating Turkish Olive Cultivar, Gemlik, Assessed By Rapd, Microsatellite, And Aflp Marker Systems, Ufuk Çeli̇kkol Akçay, Gülcan Özkan, Beki̇r Şan, Oğuz Dolgun, Ayhan Dağdelen, Di̇lşat Bozdoğan Konuşkan

Turkish Journal of Botany

Olive (Olea europaea L.) is an important fruit crop, with many health promoting properties being continuously discovered. World demand for both the fruit and olive oil is increasing in pace and hence countries try to meet the demand by disseminating 1 or 2 productive and adaptive cultivars to different regions formerly dominated by local cultivars. This study was performed to investigate the level of diversity in a very productive and adaptive cultivar, Gemlik, which has started to dominate most of the olive growing regions of Turkey. Three different marker systems, namely microsatellites (SSR), RAPD, and AFLP, with 15, 20, and …


Some Factors Affecting The Abundance Of Phytoplankton In An Unproductive Alkaline Lake (Lake Hazar, Turkey), Mehmet Ali̇ Turan Koçer, Bülent Şen Jan 2014

Some Factors Affecting The Abundance Of Phytoplankton In An Unproductive Alkaline Lake (Lake Hazar, Turkey), Mehmet Ali̇ Turan Koçer, Bülent Şen

Turkish Journal of Botany

This study aimed to explain the seasonal and vertical distribution of phytoplankton in the pelagic zone of a deep alkaline lake that is characterized by low phytoplankton abundance. Phytoplankton and water samples were collected seasonally from 9 points and 7 depths between the surface and 20 m from December 2004 to September 2005. Planktolyngbya contorta was the dominant or subdominant species in all seasons and depths. The dominance of Gomphonema species was observed in December, but they were subdominant in September. Cyclotella meneghiniana was dominant at 20 m and subdominant in the upper depths during March, June, and September. Melosira …


Heterologous Expression Of Esaba1 Enhances Salt Tolerance With Increased Accumulation Of Endogenous Aba In Transgenic Tobacco, Yujun Sun, Lin Zhu, Jiansheng Guo, Cheng Zhou Jan 2014

Heterologous Expression Of Esaba1 Enhances Salt Tolerance With Increased Accumulation Of Endogenous Aba In Transgenic Tobacco, Yujun Sun, Lin Zhu, Jiansheng Guo, Cheng Zhou

Turkish Journal of Botany

Salt stress can trigger several physiological responses in plants such as increased accumulation of ABA and antioxidant enzyme activities, which have been closely associated with the tolerance of plants to salt stress. In the present study a novel zeaxanthin epoxidase gene, designated EsABA1, was isolated from Eutrema salsugineum. EsABA1 contained a full-length open reading frame (ORF) of 2001 bp, encoding 666 amino acids. Transcription of EsABA1 rapidly accumulated after exposure to various abiotic stresses, suggesting that the EsABA1 gene was involved in multiple stress responses and tolerance. To further investigate the roles of EsABA1 under salt-stress conditions, transgenic tobacco lines …


Dreb1a From Oryza Sativa Var. Ir6: Homology Modelling And Molecular Docking, Muhammad Nawaz, Naeem Iqbal, Sobia Idrees, Ihsan Ullah Jan 2014

Dreb1a From Oryza Sativa Var. Ir6: Homology Modelling And Molecular Docking, Muhammad Nawaz, Naeem Iqbal, Sobia Idrees, Ihsan Ullah

Turkish Journal of Botany

The family of AP2/ERF plant specific transcription factors, including dehydration-responsive element binding (DREB) proteins, has been reported to activate and bind with the dehydration responsive element/C repeat (DRE/CRT) of stress-inducible gene promoters. We amplified DREB1A from Oryza sativa var. IR6 (807 bp) and subjected it to protein homology modelling and phylogenetic analysis. BLAST sequence analysis indicated high similarity (99%) with the O. sativa japonica group. Phylogenetic analysis was carried out with other 12 DREB sequences from cereals/grasses using Clustal Omega. Protein secondary structure and 3D models were determined using SOPMA and MODELLER v9.10, respectively. PROSA was used to determine the …