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Full-Text Articles in Life Sciences

The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya Apr 2023

The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya

LSU Doctoral Dissertations

Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO2 and HCO3-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO2 diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …


The Role Of Alternative Polyadenylation Mediated By Cpsf30 In Arabidopsis Thaliana, Guijie Hao Jan 2017

The Role Of Alternative Polyadenylation Mediated By Cpsf30 In Arabidopsis Thaliana, Guijie Hao

Theses and Dissertations--Plant and Soil Sciences

Drought stress is considered one of the most devastating abiotic stress factors that limit crop productivity for modern agriculture worldwide. There is a large range of physiological and biochemical responses induced by drought stress. The responses range from physiological and biochemical to regulation at transcription and posttranscriptional levels. Post-transcription, the products encoded by eukaryotic genes must undergo a series of modifications to become a mature mRNA. Polyadenylation is an important one in terms of regulation. Polyadenylation impacts gene expression through determining the coding and regulation potential of the mRNA, especially when different mRNAs from the same gene may be polyadenylated …


Trascriptome Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi Al-Azzawi May 2015

Trascriptome Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi Al-Azzawi

Theses

Signaling pathways controlling biotic and abiotic stress responses may interact synergistically or antagonistically. To identify the similarities and differences among responses to diverse stresses, we analyzed previously published microarray data on the transcriptomic responses of Arabidopsis thaliana to infection with Botrytis cinerea (a biotic stress), and to cold, drought, and oxidative stresses (abiotic stresses). Our analyses showed that at early stages after B. cinerea inoculation, 1498 genes were up-regulated (B. cinerea up-regulated genes; BUGs) and 1138 genes were down-regulated (B. cinerea down-regulated genes; BDGs). We showed a unique program of gene expression was activated in response each biotic and abiotic …


Transcriptomes Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi May 2015

Transcriptomes Analysis Reveals Genes Commonly Induced By Botrytis Cinerea Infection, Cold, Drought And Oxidative Stresses In Arabidopsis Thaliana, Ahmed Dawood Al-Azzawi

Theses

Signaling pathways controlling biotic and abiotic stress responses may interact synergistically or antagonistically. To identify the similarities and differences among responses to diverse stresses, we analyzed previously published microarray data on the transcriptomic responses of Arabidopsis thaliana to infection with Botrytis cinerea (a biotic stresses). Our analyses showed that at least at early stages B. cinerea inoculation, 1498 genes were up-regulated (B. cinerea up-regulated genes; BUGS) and 1138 genes were down-regulated (B. cinerea down-regulated genes; BUGS). We showed a unique program of gene expression was activated in response each biotic and abiotic stress, but that …


Identification Of Cyclophilin Gene Family In Soybean And Characterization Of Gmcyp1, Hemanta Raj Mainali Jul 2013

Identification Of Cyclophilin Gene Family In Soybean And Characterization Of Gmcyp1, Hemanta Raj Mainali

Electronic Thesis and Dissertation Repository

I identified members of the Cyclophilin (CYP) gene family in soybean (Glycine max) and characterized the GmCYP1, one of the members of soybean CYP. CYPs belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. PPIase catalyzes the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. After extensive data mining, I identified 62 different CYP genes in soybean (GmCYP1 to GmCYP62), of which 8 are multi-domain proteins and 54 are single domain proteins. At least 25% of the GmCYP genes are expressed in soybean. GmCYP1 …