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Flow Cytometry And Biochemical Analysis Of Apoptotic Mouse Ht-2 T - Lymphocytes, Nell Pinkston
Flow Cytometry And Biochemical Analysis Of Apoptotic Mouse Ht-2 T - Lymphocytes, Nell Pinkston
Honors Theses
Apoptosis is a highly organized intracellular death program in multicellular animals. In the vertebrate immune system, apoptosis plays a central role in preventing the emergence of autoreactive lymphocytes. In this study, we used multiple stimuli (staurosporine, camptothecin, and cytokine deprivation - interleukin-2 or IL-2) to initiate apoptosis in IL-2 dependent, mouse HT-2 T-lymphocytes. All three inducers triggered DNA laddering and phosphatidylserine externalization. Propidium iodide staining and flow cytometry were also used to determine whether apoptotic cells accumulated in a specific stage of the cell cycle, and whether the mode of induction affected cell cycle distribution. Our findings indicate that IL-2 …
Understanding A Possible Wonder Drug: A Radial Diffusion Assay For The Rapid Evaluation Of Antimicrobial Peptides, Dustin Walter
Understanding A Possible Wonder Drug: A Radial Diffusion Assay For The Rapid Evaluation Of Antimicrobial Peptides, Dustin Walter
Honors Theses
Antimicrobial peptides (AMPs) have been a major research focus due to their potential to combat a variety of human pathogens. Our laboratory has identified several novel peptides that display significant antifungal activity. The effectiveness of these peptides in vitro has been promising; however, it has been shown that physiological concentrations of various salts along with other conditions are inhibitory to peptide activity. To further explore the inhibitory effects of these salts, a new assay was developed whereby we can observe the effects of various salts on the peptide killing activity. For our studies, we employed several clinical isolates of Candida …