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Full-Text Articles in Life Sciences
Characterization Of Radiotolerance In Potato And Development Of A Gamma Radiation Phytosensor., Robert Graham Sears
Characterization Of Radiotolerance In Potato And Development Of A Gamma Radiation Phytosensor., Robert Graham Sears
Doctoral Dissertations
As humans pursue space travel and nuclear energy, the risk of harm from ionizing radiation increases. On Earth or in space, plants are essential to our personal and environmental health. Plants serve as sentinels, bioremediators and food sources in areas of high ionizing radiation, therefore it is essential to understand how ionizing radiation affects plant biology. This work aimed to understand plant responses to ionizing radiation in the potato chassis and apply that knowledge to generate novel phenotypes for nuclear energy and space applications. The first gamma radiation phytosensor was developed for monitoring at standoff distances greater than three meters. …
Cell-Free Metabolic Engineering Strategies For Accelerated Biomanufacturing, Jaime Lorenzo N. Dinglasan
Cell-Free Metabolic Engineering Strategies For Accelerated Biomanufacturing, Jaime Lorenzo N. Dinglasan
Doctoral Dissertations
Biomanufacturing propels the bioeconomy. Accelerating bioeconomic growth thus requires the expedited development of biomanufacturing processes that can expand the current bioproduct portfolio. Lysate-based cell-free systems provide unique advantages for simplified metabolic pathway construction. Their central metabolic pathways and transcriptional-translational (TX-TL) machineries are free from genome regulation and are amenable to direct manipulation, enabling the streamlined construction of biomanufacturing processes. While their utility as prototyping platforms for accelerating cellular metabolic engineering has been demonstrated, the potential to rapidly build commercial “cell-free factories” capable of sophisticated bioconversion has not been fully realized. Lysates with high-yield pathways are projected to enable commercialized cell-free …
Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk
Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk
Doctoral Dissertations
Soybean cyst nematode (SCN) is the most devastating and economically important pathogen of soybean across the United States and globally. The current primary control for SCN is with the use of resistant cultivars, however, SCN populations are shifting to overcome most common sources of soybean resistance. This research was conducted to identify novel sources of genetic resistance to SCN. First, I functionally characterized two protein kinase hub genes whose function are predicted to be essential for mediating soybean susceptibility to SCN. I generated kinase-dead variants by mutating two amino acids required for the kinase enzymatic activity. Overexpression of the kinase-dead …