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Crispr/Cas9 Genome Editing System And Its Use In Infectious Disease, Dustin Daws
Crispr/Cas9 Genome Editing System And Its Use In Infectious Disease, Dustin Daws
Thinking Matters Symposium Archive
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is an exciting new genetic engineering technology that was found in the chromosomes of certain bacteria and archaea. Bacteriophage are viruses that insert their DNA and hijack the host cell’s machinery to make new phage that can go on to infect new cells. Some microorganisms use CRISPR as a defense mechanism to disrupt the bacteriophage DNA after it is inserted into the cell. CRISPR/Cas9 uses genome editing as a means to alter very specific sections of a foreign genome. CRISPR works with a RNA-guided DNA endonuclease called Cas9. This enzyme can locate and …
A Study Of Methods For Detecting Gene Expression Of The Sterol Biosynthetic Pathway In The Microalgae Strain Heterosigma Akashiwo, Jessica C. Mayhew, Marcia Ackerman, Mike Lomas
A Study Of Methods For Detecting Gene Expression Of The Sterol Biosynthetic Pathway In The Microalgae Strain Heterosigma Akashiwo, Jessica C. Mayhew, Marcia Ackerman, Mike Lomas
Thinking Matters Symposium Archive
Biotechnological applications for various microalgae strains are beginning to bloom. There is interest in these microbes for their production of useful compounds such as phytosterols and lipids which can be produced in mass culture for large scale manufacture in the food, pharmaceutical, and biofuel industries (Romano et al., 2016). To this end, we are using the gold-brown microalgae, Heterosigma akashiwo (CCMP1680), a known producer of phytosterols, to test a molecular method for quick and easy measurements of production of the phytosterol compound sitosterol, which has potential as an anti- inflammatory agent. (Giner et al., 2008).