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Full-Text Articles in Life Sciences
Tyrosine Phosphorylation Events In Mouse Sperm Capacitation, Enid Arcelay
Tyrosine Phosphorylation Events In Mouse Sperm Capacitation, Enid Arcelay
Open Access Dissertations
Mammalian sperm are not able to fertilize immediately upon ejaculation; they become fertilization-competent after undergoing changes in the female reproductive tract collectively termed capacitation. Although it has been established that capacitation is associated with an increase in tyrosine phosphorylation, little is known about the role of this event in sperm function. In this work we used a combination of two dimensional gel electrophoresis and mass spectrometry to identify proteins that undergo tyrosine phosphorylation during capacitation. Some of the identified proteins are the mouse orthologues of human sperm proteins known to undergo tyrosine phosphorylation. Among them we identified VDAC, tubulin, PDH …
A Drosophila Resource Of Transgenic Rnai Lines For Neurogenetics, Quan Jian Ni, Lu Ping Liu, Richard Binari, Robert Hardy, Hye Seok Shim, Amanda Cavallaro, Matthew Booker, Barret D. Pfeiffer, Michele Markstein, Hui Wang, Christians Villalta, Todd R. Laverty, Lizabeth A. Perkins, Norbert Perrimon
A Drosophila Resource Of Transgenic Rnai Lines For Neurogenetics, Quan Jian Ni, Lu Ping Liu, Richard Binari, Robert Hardy, Hye Seok Shim, Amanda Cavallaro, Matthew Booker, Barret D. Pfeiffer, Michele Markstein, Hui Wang, Christians Villalta, Todd R. Laverty, Lizabeth A. Perkins, Norbert Perrimon
Michele Markstein
Conditional expression of hairpin constructs in Drosophila is a powerful method to disrupt the activity of single genes with a spatial and temporal resolution that is impossible, or exceedingly difficult, using classical genetic methods. We previously described a method (Ni et al. 2008) whereby RNAi constructs are targeted into the genome by the phiC31-mediated integration approach using Vermilion-AttB-Loxp-Intron-UAS-MCS (VALIUM), a vector that contains vermilion as a selectable marker, an attB sequence to allow for phiC31-targeted integration at genomic attP landing sites, two pentamers of UAS, the hsp70 core promoter, a multiple cloning site, and two introns. As the level of …
A New Laser Pointer Driven Optical Microheater For Precise Local Heat Shock, Mike Placinta
A New Laser Pointer Driven Optical Microheater For Precise Local Heat Shock, Mike Placinta
Masters Theses 1911 - February 2014
The zebrafish has emerged as an important genetic model system for the study of vertebrate development. However, while genetics is a powerful tool for the study of early gene functions, the approach is more limited when it comes to understanding later functions of genes that have essential roles in early embryogenesis. There is thus a need to manipulate gene expression at different times, and ideally only in some regions of the developing embryo. Methods for conditional gene regulation have been established in Drosophila, C.elegans and the mouse, utilizing conditional gene activation systems such as the Gal4-UAS system (fly) and the …