Life Sciences Commons^™

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati

Rajesh Pati

Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.

Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati

Rajesh Pati

Aegle marmelos Corr., belongs to family Rutaceae, is more prized for its pharmacological virtues than its edible quality. Because of pharmacological importance, it’s become potential candidate for developing transgenics to enhance its medicinal properties. Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants).

Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati

Rajesh Pati

A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …

Micropropagation In Fruit Crops, Rajesh Pati

Rajesh Pati

Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).

In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati

Rajesh Pati

Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).

Antifungal Activity Of Lactobacillus Plantarum Lab-C5 And Lab-G7 Isolated From Malaysian Fruits, Asma Saleh Elmabrok

Asma Saleh Elmabrok

Abstract Anthracnose disease for both seed and air borne and affects seed germination and plant vigor to a greater extent. Biological control by antagonistic microorganisms is widely recognized as a promising method for control plant diseases. This study reports the effectiveness of using lactic acid bacteria (LAB) cultures or their supernatant as a biological control against anthracnose disease in chilli caused by fungus Colletotrichum gloeosporioides. From 30 lactic acid bacteria isolated from different sources, seven isolates showed good inhibition activity against C. gloeosporioides by dual overlay method and well method. These LAB have antifungal activity grew between 10 and 45oC, …

Antifungal Activity Of Lactobacillus Plantarum Lab-C5 And Lab-G7 Isolated From Malaysian Fruits, Asma Saleh Elmabrok, Khaled M.A Hussin

Khaled M.A Hussin

Abstract Anthracnose disease for both seed and air borne and affects seed germination and plant vigor to a greater extent. Biological control by antagonistic microorganisms is widely recognized as a promising method for control plant diseases. This study reports the effectiveness of using lactic acid bacteria (LAB) cultures or their supernatant as a biological control against anthracnose disease in chilli caused by fungus Colletotrichum gloeosporioides. From 30 lactic acid bacteria isolated from different sources, seven isolates showed good inhibition activity against C. gloeosporioides by dual overlay method and well method. These LAB have antifungal activity grew between 10 and 45oC, …

Effect Of Bio Field Treatment On The Physical And Thermal Characteristics Of Silicon, Tin And Lead Powders., Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Silicon, tin and lead powders belong to group IV in periodic table and exhibit decreasing semi conducting nature towards the bottom of the group. These are very useful in producing non ferrous powder metallurgy components.

In the present investigation silicon, tin and lead powders are exposed to bio field. Both the exposed and unexposed powders are later characterized by various techniques. The average particle size, after an initial decrease is found to increase with increase in number of days after treatment although the size is lee than that exhibited by untreated powder, suggesting the operation of competing mechanisms fracture and …

Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati

Rajesh Pati

Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence …

Effect Of Bio Field Treatment On The Physical And Thermal Characteristics Of Vanadium Pentoxide Powders., Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Vanadium pentoxide powders are very useful in producing ferrous as well as aluminium alloys, in removing carbon and sulphur and as catalysts in synthesizing ammonia and sulphuric acid. It is also used as corrosion inhibitor petroleum and chemical processing.

In the present investigation V2O5 powders are exposed to biofield. Both the exposed and unexposed powders are later characterized by various techniques. The average particle size is found to decrease with increase in number of days after treatment up to a maximum of 15.9% in 110 days indicating severe fracture at agglomerate/ crystallite boundaries. The BET surface area showed a surprising …

Biofield And Fungicide Seed Treatment Influences On Soybean Productivity,Seed Quality And Weed Community, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Soybean production in Iowa USA is among the most productive for raínfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development. A field study was conducted at two locations in lowa in 2012 to determine if seed-applied fungicide or biofield treatments influenced weed community, soil volumetric water concentration and soybean yield and quality. Application of biofield treatment resulted in lower density of tall waterhemp density, greater soybean stand density at R8 stage and greater seed pod-1 compared …

Characterization Of Genes Required For Preimplantation Embryo Development, Marc P. Maserati Jr

Marc P Maserati Jr

Preimplantation embryo development in the mouse is a time of rapid cellular morphological and molecular changes leading to embryo implantation for the generation of offspring. The Mager lab studies these events occuring between fertilization and implantation in order to better understand the initial events which set the stage for all future aspects of development. The result of this research impacts many scientific disciplines including in-vitro based means of embryo culture, establishment of epigenetic marks, differentiation and cellular reprogramming and can be used in translational research for the improvement of in-vitro culture techniques and develop novel therapies such as cell replacement …

Fractal Approach To Heat Transfer In Silkworm Cocoon Hierarchy, Dong-Dong Fei, Fu-Juan Liu, Qiu-Na Cui, Ji-Huan He

Ji-Huan He

Silkworm cocoon has a complex hierarchic structure with discontinuity. In this paper, heat transfer through the silkworm cocoon is studied using fractal theory. The fractal approach has been successfully applied to explain the fascinating phenomenon of cocoon survival under extreme temperature environment. A better understanding of heat transfer mechanisms for the cocoon could be beneficial to the design of biomimetic clothes for special applications.

Microfabricated Nanotopological Surfaces For Study Of Adhesion-Dependent Cell Mechanosensitivity, Weiqiang Chen, Yubing Sun, Jianping Fu

Weiqiang Chen

Cells exhibit high sensitivity and diverse responses to the intrinsic nanotopography of the extracellular matrix through their nanoscale cellular sensing machinery. A simple microfabrication method for precise control and spatial patterning of the local nanoroughness on glass surfaces by using photolithography and reactive ion etching is reported. It is demonstrated that local nanoroughness as a biophysical cue could regulate a diverse array of NIH/3T3 fi broblast behaviors, including cell morphology, adhesion, proliferation, migration, and cytoskeleton contractility. The capability to control and further predict cellular responses to nanoroughness might suggest novel methods for developing biomaterials mimicking nanotopographic structures in vivo for …

Surface-Micromachined Microfiltration Membranes For Efficient Isolation And Functional Immunophenotyping Of Subpopulations Of Immune Cells, Weiqiang Chen, Nien-Tsu Huang, Boram Oh, Raymond H. W. Lam, Rong Fan, Timothy T. Cornell, Thomas P. Shanley, Katsuo Kurabayashi, Jianping Fu

Weiqiang Chen

An accurate measurement of the immune status in patients with immune system disorders is critical in evaluating the stage of diseases and tailoring drug treatments. The functional cellular immunity test is a promising method to establish the diagnosis of immune dysfunctions. The conventional functional cellular immunity test involves measurements of the capacity of peripheral blood mononuclear cells to produce pro-inflammatory cytokines when stimulated ex vivo. However, this “bulk” assay measures the overall reactivity of a population of lymphocytes and monocytes, making it difficult to pinpoint the phenotype or real identity of the reactive immune cells involved. In this research, we …

Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum

Sandip S. Magdum

Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …

Functions And Future Applications Of F1 Atpase As Nanobioengine - Powering The Nanoworld!, Sandip S. Magdum

Sandip S. Magdum

Recent nanotechnological revolution mandates astonishing imagination about future nanoworld. Nature has ability to create nanobiomolecules which can function in extraordinary way which can be used to produce nano hybrid systems. The opportunity to use such nanobiomolecules in combination of nanomechanical systems for development of novel nano hybrid systems for their various applications needs to explore in further nanotechnological development. F1 ATPase is a subunit of ATP synthase, which is one of the biomolecular structure works on the plasma membrane of the living cell. The reversible function of F1 ATPase gives a counterclockwise rotation of γ shaft by hydrolyzing ATP and …

An Efficient Biotreatment Process For Polyvinyl Alcohol Containing Textile Wastewater, Sandip S. Magdum, Gauri P. Minde, Upendra S. Adhyapak, Kalyanraman V.

Sandip S. Magdum

The aim of this work was to optimize the biodegradation of polyvinyl alcohol (PVA) containing actual textile wastewater for a sustainable treatment solution. The isolated microbial consortia of effective PVA degrader namely Candida Sp. and Pseudomonas Sp., which were responsible for symbiotic degradation of chemical oxidation demand (COD) and PVA from desizing wastewater. In the process optimization, the maximum aeration was essential to achieve a high degradation rate, where as stirring enhances further degradation and foam control. Batch experiments concluded with the need of 16 lpm/l and 150 rpm of air and stirring speed respectively for high rate of COD …

Biogas As A Sustainable Alternative For Current Energy Need Of India., Gauri P. Minde, Sandip S. Magdum, Kalyanraman V.

Sandip S. Magdum

Per capita energy consumption of India is declining with increasing its population, which has direct impact on national economy. Biogas technology seems promising to attain sustainable energy yields without damaging the environment. Waste management, manure creation, health care and employment foundation are the benefits of biogas system. Use of biogas assures renewable energy supply and balance of green house gases. India is traditionally using biogas since long time but there is need to improve the technology, applications and deployment strategies. Bioenergy centralization in urban and decentralization in rural can help government to minimize both the import of fuel derivatives and …

Development Of A Novel Inoculum Preparation Method For Solid-State Fermentation - Cellophane Film Culture (Cfc) Technique, Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua

Teck Nam Ang Dr.

This study reports a user-friendly technique in the preparation of fungal inoculum intended for solid-state fermentation (SSF) – Cellophane Film Culture (CFC) technique. This technique uses cellophane film-overlaid agar plates to facilitate the separation of fungal biomass. The findings showed that inoculum of P. sajor-caju produced is viable, and it was confirmed by the presence of laccase enzyme activity in SSF of rice husk. The correlation between fungal dry and wet weights (r2 = 0.9329) provides an accurate estimation of fungal dry weight from its wet weight during inoculum preparation. Besides, this technique does not require a strict sterile handling …

Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.

Halijah Ibrahim

BACKGROUND: Alpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is used by the locals to treat flatulence. In this study, the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five different cancer and one normal cell lines were investigated. The total phenolic content of each extract and its fractions were also quantified. This is the first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract. METHODS: In the current study, the crude methanol and fractionated extract of the rhizomes of Alpinia pahangensis were investigated for their …

The New Frontier Of Advanced Reproductive Technology: Reevaluating Modern Legal Parenthood, Yehezkel H. Margalit Dr., John D. Loike Dr., Orrie Levy Adv.

Hezi Margalit

Assisted reproductive technologies (ARTs) have challenged our deepest conceptions of what it means to be a parent by fragmenting traditional aspects of parenthood. The law has been slow to respond to this challenge, and numerous academic articles have proposed models for adapting parentage laws to ARTs. In the coming years, however, scientific advancements in reproductive technologies, such as somatic cell nuclear transfer and stem cell technologies, will challenge both parentage laws and proposed legal models for traditional ARTs in new and fascinating ways. For instance, these advanced technologies could allow two women to create a child without any male genetic …