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Full-Text Articles in Life Sciences
Initial Characterization Of The Large Genome Of The Salamander Ambystoma Mexicanum Using Shotgun And Laser Capture Chromosome Sequencing, Melissa C. Keinath, Vladimir A. Timoshevskiy, Nataliya Y. Timoshevskaya, Panagiotis A. Tsonis, S. Randal Voss, Jeramiah J. Smith
Initial Characterization Of The Large Genome Of The Salamander Ambystoma Mexicanum Using Shotgun And Laser Capture Chromosome Sequencing, Melissa C. Keinath, Vladimir A. Timoshevskiy, Nataliya Y. Timoshevskaya, Panagiotis A. Tsonis, S. Randal Voss, Jeramiah J. Smith
Biology Faculty Publications
Vertebrates exhibit substantial diversity in genome size, and some of the largest genomes exist in species that uniquely inform diverse areas of basic and biomedical research. For example, the salamander Ambystoma mexicanum (the Mexican axolotl) is a model organism for studies of regeneration, development and genome evolution, yet its genome is ~10× larger than the human genome. As part of a hierarchical approach toward improving genome resources for the species, we generated 600 Gb of shotgun sequence data and developed methods for sequencing individual laser-captured chromosomes. Based on these data, we estimate that the A. mexicanum genome is ~32 Gb. …
The Sea Lamprey Meiotic Map Improves Resolution Of Ancient Vertebrate Genome Duplications, Jeramiah James Smith, Melissa C. Keinath
The Sea Lamprey Meiotic Map Improves Resolution Of Ancient Vertebrate Genome Duplications, Jeramiah James Smith, Melissa C. Keinath
Biology Faculty Publications
It is generally accepted that many genes present in vertebrate genomes owe their origin to two whole-genome duplications that occurred deep in the ancestry of the vertebrate lineage. However, details regarding the timing and outcome of these duplications are not well resolved. We present high-density meiotic and comparative genomic maps for the sea lamprey (Petromyzon marinus), a representative of an ancient lineage that diverged from all other vertebrates ~550 million years ago. Linkage analyses yielded a total of 95 linkage groups, similar to the estimated number of germline chromosomes (1n ~ 99), spanning a total of 5570.25 cM. …