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Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport
Mapping Genetic Variants Associated With Dynamic Protein Abundance In Haploid Yeast, Tanner Thuet-Davenport
WWU Graduate School Collection
As organisms respond to changes in their environment genetic variation between individuals can directly affect organismal trait phenotypes by altering gene expression. Historically, studies have focused on the effect of genetic variation on mRNA synthesis (transcription) and decay rates. Relatively few studies have probed the relationship between DNA variants and protein-specific regulation of individual genes, despite the plethora of evidence that RNA levels are often poor proxies for protein levels. No study to date has mapped genetic variation associated with dynamic protein levels. In this study we investigated the location and identity of genetic variants acting on protein expression dynamics …
Codon Bias And Mrna Folding Stability: Two Natural Controls Of Protein Expression Dynamics, Anastacia Wienecke
Codon Bias And Mrna Folding Stability: Two Natural Controls Of Protein Expression Dynamics, Anastacia Wienecke
WWU Graduate School Collection
Introduction: Connections between genetic variation and trait variation are complex and dynamic; the critical link between the two is gene expression variation. Though proteins are the functional products of most genes, the relative ease and throughput level of various measurement approaches has meant that gene expression is typically studied via transcript-level rather than protein-level techniques. Recent studies however, suggest that certain genetic factors act post-transcriptionally to modify rates of protein synthesis, making transcript levels imperfect indicators of protein levels. A gene’s bias for ‘optimal’ codons (i.e., its codon bias) and a gene’s mRNA folding stability appear to be two such …