Life Sciences Commons^™

The Neuronal Calcium Sensor Ncs-1 Regulates The Phosphorylation State And Activity Of The Gα Chaperone And Gef Ric-8a, Daniel Muñoz-Reyes, Levi J. Mcclelland, Sandra Arroyo-Urea, Sonia Sánchez-Yepes, Juan Sabín, Sara Pérez-Suárez, Margarita Menendez, Alicia Mansilla, Javier García-Nafría, Stephen Sprang, Maria Jose Sanchez-Barrena

Biological Sciences Faculty Publications

The neuronal calcium sensor 1 (NCS-1), an EF-hand Ca binding protein, and Ric-8A coregulate synapse number and probability of neurotransmitter release. Recently, the structures of Ric-8A bound to Gα have revealed how Ric-8A phosphorylation promotes Gα recognition and activity as a chaperone and guanine nucleotide exchange factor. However, the molecular mechanism by which NCS-1 regulates Ric-8A activity and its interaction with Gα subunits is not well understood. Given the interest in the NCS-1/Ric-8A complex as a therapeutic target in nervous system disorders, it is necessary to shed light on this molecular mechanism of action at atomic level. We have reconstituted …

Monocytes And Macrophages Promote Increased Tissue Factor-Factor Viia-Dependent Procoagulant Activity In Response To The Sars-Cov-2 Spike Protein, James Harte

ORBioM (Open Research BioSciences Meeting)

Background

Venous thromboembolism (VTE) is a high-incidence complication of severe SARS-CoV-2-associated COVID-19. Recently, abnormal tissue factor expression has been linked to thromboembolic events in patients with COVID-19. Monocytes and macrophages are the predominant source of tissue factor in peripheral circulation, which suggests a potential pathomechanistic interplay between SARS-CoV-2 and the monocyte-macrophage axis. However, the link between monocytes and macrophages and the thromboembolic risk associated with COVID-19 is largely unaddressed.

Aims

In this study, we investigated changes in monocyte/macrophage procoagulant activity in response to the SARS-CoV-2 spike protein.

Methods

Immortalised human-derived monocytes and differentiated macrophages were treated with the SARS-CoV-2 spike …

Doxorubicin-Induced Modulation Of Tgf-Β Signaling Cascade In Mouse Fibroblasts: Insights Into Cardiotoxicity Mechanisms, Conner Patricelli, Parker Lehmann, Julia Thom Oxford, Xinzhu Pu

Biology Faculty Publications and Presentations

Doxorubicin (DOX)-induced cardiotoxicity has been widely observed, yet the specific impact on cardiac fibroblasts is not fully understood. Additionally, the modulation of the transforming growth factor beta (TGF-β) signaling pathway by DOX remains to be fully elucidated. This study investigated DOX’s ability to modulate the expression of genes and proteins involved in the TGF-β signaling cascade in mouse fibroblasts from two sources by assessing the impact of DOX treatment on TGF-β inducible expression of pivotal genes and proteins within fibroblasts. Mouse embryonic fibroblasts (NIH3T3) and mouse primary cardiac fibroblasts (CFs) were treated with DOX in the presence of TGF-β1 to …