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Dapi Staining And Dna Content Estimation Of Nuclei In Uncultivable Microbial Eukaryotes (Arcellinida And Ciliates), Ketty Munyenyembe, Caitlin Timmons, Agnes K.M. Weiner, Laura A. Katz, Ying Yan

Biological Sciences: Faculty Publications

Though representing a major component of eukaryotic biodiversity, many microbial eukaryotes remain poorly studied, including the focus of the present work, testate amoebae of the order Arcellinida (Amoebozoa) and non-model lineages of ciliates (Alveolata). In particular, knowledge of genome structures and changes in genome content over the often-complex life cycles of these lineages remains enigmatic. However, the limited available knowledge suggests that microbial eukaryotes have the potential to challenge our textbook views on eukaryotic genomes and genome evolution. In this study, we developed protocols for DAPI (4′,6-diamidino-2-phenylindole) staining of Arcellinida nuclei and adapted protocols for ciliates. In addition, image analysis …