Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 15 of 15

Full-Text Articles in Life Sciences

Isolation And Identification Of The Fungus Monascus Purpureus From Imported Currants As A New Record In Iraq, Ghasaq Malik Al-Barkawie, A.S. Saadon Oct 2021

Isolation And Identification Of The Fungus Monascus Purpureus From Imported Currants As A New Record In Iraq, Ghasaq Malik Al-Barkawie, A.S. Saadon

Al-Qadisiyah Journal of Pure Science

This study was conducted in the Laboratory of Fungus in the Department of Biology / College of Science / University of Qadisiyah to isolate and diagnose some insulation from fungi isolated from imported dried fruits (raisins) in Qadisiyah province, Iraq. The isolations were diagnosed both morphologically and microscopically using the classification keys and to confirm the appearance and microscopic diagnosis diagnosed using polymerase chain reaction(PCR), And determine the sequence of nitrogen bases (Nucleotide sequence(of compound DNA products using ITS1 and ITS4. The results of the nucleotide sequence analysis of DNA (PCR product) compounding innate isolation and using BLAST to compare …


Environmental And Taxonomic Study Of Mosquitoes In Al-Dywaniyah Governorate, Hanaa Rahman Lefta Oct 2021

Environmental And Taxonomic Study Of Mosquitoes In Al-Dywaniyah Governorate, Hanaa Rahman Lefta

Al-Qadisiyah Journal of Pure Science

The current study included the collection and classification of mosquitoes and the identification of some species because of their medical and veterinary importance in terms of their harmful effect on health and their transmission of diseases. Three species of mosquitoes were diagnosed in the different study sites, namely Anopheles, Culex and Aedes, where five types of Anopheles and four types of Culex were recorded. As for the third genus, two types of it were recorded, and the proportions of the types collected from the study sites varied among themselves, as the percentages varied according to the day and night and …


The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq, Marwa Subhi Ibrahim, Haval Mohammed Khalid, Wijdan M. S. Mero Oct 2021

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq, Marwa Subhi Ibrahim, Haval Mohammed Khalid, Wijdan M. S. Mero

Al-Qadisiyah Journal of Pure Science

This study involved the prevalence of uropathogenic Escherichia coli (UPEC) among outpatients of UTI attending three major hospitals in Zakho city. Four hundred urine samples were collected from patients of UTI of both sexes and different ages (≤ 1 year to over 50 years), during the period from July 2018 until January 2019. All urine samples were analyzed by conventional bacteriological method for the presence of Escherichia coli (E. coli), while molecular method was used for the presence of species-specific uidA gene in the isolated E. coli. Out of 400 samples, 141 (35.25%) were infected with UPEC from enrolled patients. …


A Identification And Isolation Of Several Bacteria From Oral Cavity Among Human In Soran City, Holem Hashm Balaky, Alaa Taha Younis Al-Hammadi Oct 2021

A Identification And Isolation Of Several Bacteria From Oral Cavity Among Human In Soran City, Holem Hashm Balaky, Alaa Taha Younis Al-Hammadi

Al-Qadisiyah Journal of Pure Science

The aim of this study has been to estimate that the number of bacteria in the mouth exceeds the total number of people on the planet. In this context, scientists have discovered more than 700 different types of human oral bacteria. With such a large number of oral bacteria present, many diseases that may threaten health, especially oral health, can emerge. For this reason, this study was employed and conducted on 252 healthy people in the Soran area of the city of Erbil. Both sexes were involved and different ages were selected. Swabs taken from 226 participants gave positive growths …


Larvicidal Effect Of Secondary Metabolites Of Three (Diptera:Culicidae)Fungi Againstcx.Quinquefasciatus, Ali A. H. Abdul Al-Ameer Jul 2021

Larvicidal Effect Of Secondary Metabolites Of Three (Diptera:Culicidae)Fungi Againstcx.Quinquefasciatus, Ali A. H. Abdul Al-Ameer

Al-Qadisiyah Journal of Pure Science

The larvicidal effect of secondary metabolites of Verticillum lecanii,Pencillium chrysogenum and Aspergillus niger against filaria vector Cx.quinquefasciatus was investigated. efficacies have been tested at four different concentrations of secondary metabolites of mentioned fungi (25,50,75,100)ppm .larvicidal efficacy demonstrates that larval mortality was related to concentration, exposure time and larval age. Higher mortality was observed at high concentration, moreover mortality increased when exposure time increased , additionally , the older larvae were more resistant to fungal filtrate. Also, it was perceptible that larvae of Cx.quinquefasciatus have a chieved higher mortality for V.lecanii, at the concentration (100ppm )after 24 ,48,and 72 hrs for …


Therapeutic Plants Used For Typhoid Fever Treatment In Kaduna State Nigeria, Mahmoud Dogara Abdulrahman, Saber W. Hamad, Muhammad Usman, Salisu Muhammad Tahir, Namadi Sunusi, Abdulkadir Yunusa Jul 2021

Therapeutic Plants Used For Typhoid Fever Treatment In Kaduna State Nigeria, Mahmoud Dogara Abdulrahman, Saber W. Hamad, Muhammad Usman, Salisu Muhammad Tahir, Namadi Sunusi, Abdulkadir Yunusa

Al-Qadisiyah Journal of Pure Science

For ages, medicinal plants play a significant role in the effective, affordable, and safe healthcare system. Medicinal plants continue to play an important role in Nigeria's healthcare system, which serves more than 70% of the country's population. For pharmaceutical exploration and conservation, it is important to document the use of medicinal plants in a specific region across time. The study's goal was to find out which plants were used to treat typhoid illness in Kaduna state. The in-depth interview guide was utilized in conjunction with an expert method and non-random probability sampling. The study discovered and recorded 31 plant species …


Department Of Basic And Applied Science, Federal Polytechnic Mubi, Adamawa State, Nigeria, Hameed Rashid Ahmed, Hazhar Mohamed Aziz Jul 2021

Department Of Basic And Applied Science, Federal Polytechnic Mubi, Adamawa State, Nigeria, Hameed Rashid Ahmed, Hazhar Mohamed Aziz

Al-Qadisiyah Journal of Pure Science

During present study, 30 samples of drinking water from five different sources of drilled well from Kifry city have been collected and investigated for the levels of their physiochemical and Microbiological Coliform. Chemical tests were performed include tests of some soluble inorganic ions, such as chloride (Cl1-) and calcium (Ca2+), potassium (K+) sodium (Na+), magnesium (Mg2+). While the conductivity, pH, total dissolved solid (TDS), and turbidity tests belong to the physical tests. The results of chemical and physical test demonstrate the water of drilled wells in study area is possible for drinking according to Iraqi standard limits for drinking water. …


Study Molecular And Immunological Techniques For Detection Of Leishmania Tropical, Major In Baghdad , Iraq, Aliaa Faiq Al-Naimy, Ali B. M. Al-Waaly Apr 2021

Study Molecular And Immunological Techniques For Detection Of Leishmania Tropical, Major In Baghdad , Iraq, Aliaa Faiq Al-Naimy, Ali B. M. Al-Waaly

Al-Qadisiyah Journal of Pure Science

The study was conducted on 444 patients suffering from skin lesions of different ages and genders that were diagnosed by dermatologists as cutaneous leishmaniasis for people reviewing and throughout the days of the week for the department of epidemiology and transitional diseases who attended to hospitals in the city of Baghdad, namely (Al-Shaheed Dari Al Fayyad Teaching Hospital , Al-Shaheed Al Sadr Hospital , Al-Numan Teaching Hospital, Al-Karama Teaching Hospital) for the study period from October 1, 2019 until the end of March 2020. The methods of molecular diagnostics showed that multiplex pcr examined 40 random samples of the parasite …


Histopathological Effects Of Carbamazepine On The Reproductive System Of Male Rats, Hayder A. Majeed Apr 2021

Histopathological Effects Of Carbamazepine On The Reproductive System Of Male Rats, Hayder A. Majeed

Al-Qadisiyah Journal of Pure Science

This research aims to identify the effect of carbamazepine on genital tissues of male rats. In this experiment (20) male from adult rats were randomly assigned to 2 groups, Each group comprises (10) animals. Control group gavage with distilled water, First group gavage carbamazepine concentration (30) mg/kg of body weight. After 45 days, genitals eradicated for the purpose of textile on study them, Histological examination showed pathological changes in the occurrence of the testis in (T1) represented by its small diameter tubular deferens Also, the number of cells formed for sperm cells and spermatid and leydig cells has been reduced …


Effect Of Some Plants Extracts On The Mortality Of The 4th Larval Instar Mosquitoes Culex Quinquefasciatus, Areej Hasan S. Al-Dhaher Apr 2021

Effect Of Some Plants Extracts On The Mortality Of The 4th Larval Instar Mosquitoes Culex Quinquefasciatus, Areej Hasan S. Al-Dhaher

Al-Qadisiyah Journal of Pure Science

In this study, the effect of water and alcoholic (ethanol) extracts of Caper Bush Capparis spinosa L., Cumine Cuminum cyminum L. , Black pepper Piper nigrum L., Sage Salvia sp. and Cat thyme Teucrium polium L. plants on the 4th instar larvae of Culex quinquefasciatus have been evaluated .The results showed that the water extract of P. nigrum had the highest effect on the larvae, with 48h LC50 155. 9 ppm followed by ethanol extracts of C. cyminum , C. spinosa and P. nigrum with 48h LC50 308.2 ,315.1and 791.4 ppm respectively. For all studied plants, the secondary compounds were …


Detection Of Β-Lactamases Enzymes Responsible For Β-Lactam Resistance In Some Gram Negative Bacteria By Pcr, Rawa Abdul Redha Aziz, Sura Alaa Saud, Jinan Azeez Thabit Apr 2021

Detection Of Β-Lactamases Enzymes Responsible For Β-Lactam Resistance In Some Gram Negative Bacteria By Pcr, Rawa Abdul Redha Aziz, Sura Alaa Saud, Jinan Azeez Thabit

Al-Qadisiyah Journal of Pure Science

Objective(s): β- lactamases enzymes are well studied in literature since they lead to β-lactam resistance among many pathogens, hence global concern of human and animal health issues is rising. The aim of study is to detect these kinds of enzymes among some gram negative bacteria isolated locally. Material and methods: 30 isolate of each gram negatives Citrobacter spp., Serratia spp., and Enterobacter spp. were obtained from different clinical samples in Baghdad and Al-Najaf cities through March 2020 until January 2021. Isolates were identified biochemically and by Vitek2 system. β- Lactamases encoding genes like (blaSHV (231bp), blaIMP-1 (500), blaVIM (382), blaAmp …


Antibiogram Of Klebsiella Pneumoniae That Isolated From Clinical And Environmental Samples In Al-Diwaniyah Hospitals, Zahraa Ahmed Abd Al-Hamed, Firas Srhan Abd Al-Mayahi Apr 2021

Antibiogram Of Klebsiella Pneumoniae That Isolated From Clinical And Environmental Samples In Al-Diwaniyah Hospitals, Zahraa Ahmed Abd Al-Hamed, Firas Srhan Abd Al-Mayahi

Al-Qadisiyah Journal of Pure Science

Antibiotic resistance in bacteria has become a worrying phenomenon in today's world, K. pneumoniae is a member of the Enterobacteriaceae family, which causes nosocomial infections as an opportunistic pathogen but inherently harbored as a part of the natural human microbiota this study aims to inform the sensitivity of bacteria to different antibiotics commonly used in the treatment against K. pneumoniae strains isolated from different infection sites and hospital environmental samples. Samples were collected from different sources (n = 886) were recorded from Diwaniyah hospitals during the period from August 2019 to January 2020, Among the 275 isolates positive for bacterial …


Detection Of Some Virulence Enzymes Of Malasseziaspp. Isolated From Patients Of Pityriasis Versicolor And Their Sensitivity To Some Antifungal Agents, Abeer T. Noor, Ali A. Alsudani Jan 2021

Detection Of Some Virulence Enzymes Of Malasseziaspp. Isolated From Patients Of Pityriasis Versicolor And Their Sensitivity To Some Antifungal Agents, Abeer T. Noor, Ali A. Alsudani

Al-Qadisiyah Journal of Pure Science

This study included isolation and identification of Malassezia spp. from patients of Pityriasis Versicolor (PV) diagnosis by the dermatological consultant at Al-Diwaniyah Teaching Hospital for the period from 1/10/2019 to 5/3/2020, and detection of the virulence enzymes of these species and their sensitivity to some antifungal agents. 87 specimens were collected (38 females 43.7%, and 49 males 56.3%). 73 specimens (84%) were positive while 14 specimens (16%) were negative to direct and indirect examination. The results of isolation and identification of Malassezia spp. based on direct examination of specimens with KOH10%, indirect methods of growing specimens on SDA medium, and …


Intestinal Escherichia Coliand Cutaneous Staphylococcus Aureus Bacterial Contamination Of The Operation Lounges In Al-Diwaniyah Province Hospitals, Salwan A. Abdulsahib, Fallah H. Abdullatef, Bassim M. Al-Khafaji, Bassim M. Al-Khafaji, Anwar H. Jawad, Qassim J. Talib Jan 2021

Intestinal Escherichia Coliand Cutaneous Staphylococcus Aureus Bacterial Contamination Of The Operation Lounges In Al-Diwaniyah Province Hospitals, Salwan A. Abdulsahib, Fallah H. Abdullatef, Bassim M. Al-Khafaji, Bassim M. Al-Khafaji, Anwar H. Jawad, Qassim J. Talib

Al-Qadisiyah Journal of Pure Science

The research aims to study the bacterial contamination of the two types, E.coli and St.aureus, which are endemic in the lobbies and halls of operations and childbirth in the hospitals of Al-Diwaniyah province, where 4475 samples were isolated from governmental and private hospitals throughout the province from October 2019 to September 2020, where The study included taking samples from six hospitals in the city center and three in the districts and sub-districts of the governorate. (20.26%), Women's and Children's Hospital (19.74%), Al-Diwaniyah Teaching Hospital (19.33%), Hamza Hospital (17.64%), the Middle Euphrates Al-Ahli 13.02, and finally Al-Diwaniyah National Hospital (4.92%), which …


Seroprevalence And Risk Factors Of Toxoplasma Gondiiamong Children In Al-Qadisiyah Province –Iraq, Musafer H. Al-Ardi Jan 2021

Seroprevalence And Risk Factors Of Toxoplasma Gondiiamong Children In Al-Qadisiyah Province –Iraq, Musafer H. Al-Ardi

Al-Qadisiyah Journal of Pure Science

Background: Toxoplasmosis is a disease associated with the nature of the population, domestic quality and general health culture. Toxoplasma gondii causes many healthy and psychological problems with the possibility of transmission from the mother to her fetus. The primary objective of this study is to establish a database of infection rates among the governorate's children, with the absence of a database of the parasite prevalence rate among children. Materials and method: Four hundred sixty-three serum samples from children 13 old and under were collected during the period from 6 Jan. 2019 to 12 Dec. 2019, all of which were examined …