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The Development Of A Tractable System To Assess Caulobacter-Plant Interactions, Louis Berrios
The Development Of A Tractable System To Assess Caulobacter-Plant Interactions, Louis Berrios
Theses and Dissertations
Bacteria play an integral role in regulating plant growth and development. However, many of the mechanisms encompassing bacteria-plant interactions are poorly understood and thus require detailed assessments (see CHAPTER 1). To this end, I coupled bacterial (Caulobacter sp.) and plant model organisms (Arabidopsis) to determine 1) the degree to which select bacteria can enhance the growth and development of plants, and 2) what functions these bacteria possess that enable them to aid plant development. Employing bacterial isolation techniques, monoculture inoculum-based plant growth assays, biochemical assays, comparative genomics, functional genetics, and real-time quantitative PCR (RT-qPCR), I determined that …
Plasmids Bring Novel Functions To Caulobacter Genomes, Taylor Howell Carter
Plasmids Bring Novel Functions To Caulobacter Genomes, Taylor Howell Carter
Theses and Dissertations
Caulobacter is a well-studied bacterial genus, but little is known about the plasmids that are found in some wild Caulobacter isolates. I identified nine plasmids from seven different Caulobacter strains and grouped them based on their size and the similarity of their repABC, parAB, and mobAB genes. Protein pathway analysis of the genes on the K31p1, and K31p2 plasmids showed many metabolic pathways that would enhance the metabolic versatility of the host strain. In contrast, the CB4 plasmid contained 21 heavy metal resistance genes organized into 9 operons. The majority of the CB4 heavy metal resistance genes code for …