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Biology

2021

University of South Carolina

Bacteria-plant

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The Development Of A Tractable System To Assess Caulobacter-Plant Interactions, Louis Berrios Jul 2021

The Development Of A Tractable System To Assess Caulobacter-Plant Interactions, Louis Berrios

Theses and Dissertations

Bacteria play an integral role in regulating plant growth and development. However, many of the mechanisms encompassing bacteria-plant interactions are poorly understood and thus require detailed assessments (see CHAPTER 1). To this end, I coupled bacterial (Caulobacter sp.) and plant model organisms (Arabidopsis) to determine 1) the degree to which select bacteria can enhance the growth and development of plants, and 2) what functions these bacteria possess that enable them to aid plant development. Employing bacterial isolation techniques, monoculture inoculum-based plant growth assays, biochemical assays, comparative genomics, functional genetics, and real-time quantitative PCR (RT-qPCR), I determined that …