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Full-Text Articles in Life Sciences

Testing The Role Of Wfs1 In Calcium Regulation In Drosophila Cells, Briana Scott, Alyandra Lee Nov 2021

Testing The Role Of Wfs1 In Calcium Regulation In Drosophila Cells, Briana Scott, Alyandra Lee

Fall Showcase for Research and Creative Inquiry

The purpose of the project is to knock out the Wfs1 gene in Drosophila (fruit fly) cells and test the hypothesis that this gene is involved in regulating intracellular calcium levels. To test this hypothesis, the gene will first be knocked out using CRISPR-Cas9 molecular tools that are readily available in Dr. Shanle's lab. This will effectively inactivate the Wsf1 gene in cells. To do this, we will transfect Drosophila cells with CRISPR-Cas9 targeting the gene. Next, we will confirm that the gene is mutated using genomic DNA purification, polymerase chain reaction, and sequencing. Finally, we will treat cells with …


Reconstructing Isotelus Rex, Brian White Nov 2021

Reconstructing Isotelus Rex, Brian White

Fall Showcase for Research and Creative Inquiry

Isotelus rex is the largest trilobite currently known at over 720 mm in length, and by extension is one of the largest arthropods known. However, despite these accolades, it is relatively unknown in both the public consciousness and in the scientific community. This is partially due to its scant visual database, with barely any artistic restorations to help represent its life appearance. In order to rectify this, a digital reconstruction of Isotelus rex is produced utilizing programs such as Blender and Zbrush. The final result of the work is a fully animated restoration of the trilobite, bolstering its visual database …


Can Magic Mushrooms Remove Inherited Trauma?, Caleb Edwards Nov 2021

Can Magic Mushrooms Remove Inherited Trauma?, Caleb Edwards

Fall Showcase for Research and Creative Inquiry

This presentation is about a proposal wherein mice are exposed to psilocybin over multiple generations to study the effects of psilocybin on fear response, hippocampal growth and gene expression.


Scanning Electron Microscopic Analysis Of Developmental Speed's Effect On Symmetry In Drosophila Melanogaster, Kyle Hiatt Nov 2021

Scanning Electron Microscopic Analysis Of Developmental Speed's Effect On Symmetry In Drosophila Melanogaster, Kyle Hiatt

Fall Showcase for Research and Creative Inquiry

The purpose of this project is primarily to examine the effects of development speed on symmetry in Drosophila melanogaster when employing methylparaben, an anti-microbial preservative, as an additive to the organisms' growth medium. This paraben was selected due to its studied effects on prolonging the length of juvenile development. The research question being examined in this project is whether manipulating developmental speed by lengthening the average developmental cycle would lead to more overall symmetry as opposed to a shorter developmental cycle speed. Baseline assays of juvenile developmental speed of Drosophila melanogaster, both groups with and without methylparaben growth medium, will …


Why Is It Beneficial For Crustaceans To Become Crab-Like?, Dylan Blanton Nov 2021

Why Is It Beneficial For Crustaceans To Become Crab-Like?, Dylan Blanton

Fall Showcase for Research and Creative Inquiry

There are two types of crabs, true crabs and false crabs. The true crabs, Brachyurans, evolved from the original lineage and false crabs, Anomurans, have evolved from separate lineages. The main research plan is to analyze the use of each morphological feature that is similar between the Brachyurans and Anomurans and identify if it provides an edge over other competitive organisms that do not exhibit that trait. This is planned to be studied by identifying and observing if certain crab-like morphological traits provide any advantage over organisms without those traits. This will be executed by finding several locations associated with …


Risk Assessment Of Blackbird Mine, Tinsae Alem Nov 2021

Risk Assessment Of Blackbird Mine, Tinsae Alem

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Blackbird Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Mohawk Tannery, Sarah Roquemore Nov 2021

Risk Assessment Of Mohawk Tannery, Sarah Roquemore

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Mohawk Tannery. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Unity Auto Mart, Ashley Wilson Nov 2021

Risk Assessment Of Unity Auto Mart, Ashley Wilson

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Unity Auto Mart. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


The Effect Of Weight On The Amount Of Force On Ankle Joints During A Vertical Jump, Layne Fadely, Isabella Riccoboni, Stephanie Willis Nov 2021

The Effect Of Weight On The Amount Of Force On Ankle Joints During A Vertical Jump, Layne Fadely, Isabella Riccoboni, Stephanie Willis

Fall Showcase for Research and Creative Inquiry

Ankles are one of the most complex parts of the human body. The ankle consists of bones, muscles, and joints that all work together to carry the weight of the human body, meaning the ankle also faces the most pressure and injuries. The main goal of this class project and what we are trying to determine is whether or not weight is a factor that effects the amount of force that the ankle takes on when jumping. The angles of the ankles are also being monitored due to the fact that weight could also have a correlation with them. We …


An Investigation Into The Effect Of Polluted Bodies Of Water On The Food Source Of Shore Birds, Ian Castelow Nov 2021

An Investigation Into The Effect Of Polluted Bodies Of Water On The Food Source Of Shore Birds, Ian Castelow

Fall Showcase for Research and Creative Inquiry

The goal or specific aim of this research is to gain better knowledge into how big of an effect heavy metal pollution is having on the not only the environment but also shorebird species and their food source. Shorebirds are a great climate change indicator and with this new knowledge we can show why they need to be protected even more.


Risk Assessment Of Vaping, Gillian Eshelman Nov 2021

Risk Assessment Of Vaping, Gillian Eshelman

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for vaping. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Blackbird Mine, Eli Caldwell Nov 2021

Risk Assessment Of Blackbird Mine, Eli Caldwell

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Blackbird Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Microbial Diversity Of Human-Cultivated And Wild Soil On Longwood University's Campus, Cassandra Poole, Jack Kloeppel Nov 2021

Microbial Diversity Of Human-Cultivated And Wild Soil On Longwood University's Campus, Cassandra Poole, Jack Kloeppel

Fall Showcase for Research and Creative Inquiry

Soil pH is one of the biggest determinants for microbial diversity and composition. Many human interventions in soil chemistry involve processes that alter the pH. On Longwood University's campus, some regions are cultivated under a nutrient management plan. In this study, the microbial diversity of these human-cultivated regions was compared to that of wild regions. In general, it was found that the diversity of human-cultivated regions was higher than that of wild regions.


Risk Assessment Of Pcb Exposure From Mvp In Southwest, Va, Elizabeth Garri Nov 2021

Risk Assessment Of Pcb Exposure From Mvp In Southwest, Va, Elizabeth Garri

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for PCB exposure from MVP in Southwest Virginia. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Bear Creek Sediments, Rebekah Godfrey Nov 2021

Risk Assessment Of Bear Creek Sediments, Rebekah Godfrey

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Bear Creek Sediments. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Anaconda Copper Mine, Kelly Jarratt Nov 2021

Risk Assessment Of Anaconda Copper Mine, Kelly Jarratt

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Anaconda Copper Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Millsboro, De Trichloroethylene Contamination, Rebecca Mills Nov 2021

Risk Assessment Of Millsboro, De Trichloroethylene Contamination, Rebecca Mills

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Millsboro, DE Trichloroethylene Contamination. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Yellow Pine Mining Area, Noel Preece Nov 2021

Risk Assessment Of Yellow Pine Mining Area, Noel Preece

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Yellow Pine Mining Area. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Risk Assessment Of Devil's Swamp Lake, Jocelyn Price Nov 2021

Risk Assessment Of Devil's Swamp Lake, Jocelyn Price

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Devil's Swamp Lake. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.


Microbial Diversity Of Rural And Urban Soil At Different Depths On Longwood University's Campus, Kristen Dobek, Madalyn Cook Nov 2021

Microbial Diversity Of Rural And Urban Soil At Different Depths On Longwood University's Campus, Kristen Dobek, Madalyn Cook

Fall Showcase for Research and Creative Inquiry

Microbial diversity is the range of various kinds of bacteria. The purpose of this experimental research was to determine the complex diversity of bacteria among soil samples at two locations on Longwood University's campus. The study found that there was a higher microbial diversity in the rural soil sample location compared to the urban soil sample location.


Does Playing Video Games Make You An Athlete?, Taylor Murphy, Briana Scott, Alyandra Lee, Katelin Savage Nov 2021

Does Playing Video Games Make You An Athlete?, Taylor Murphy, Briana Scott, Alyandra Lee, Katelin Savage

Fall Showcase for Research and Creative Inquiry

The purpose of this study is to compare the tap and move-and-tap reaction times between those who play sports, those who play video games, and those who do neither. We hypothesize that the "Neither" group will have the slowest reaction times, while the "Sports" group will have the fastest move-and-tap reaction times, and the "Gamers" group has the fastest tap reaction times. Many research articles have indicated that gamers and athletes have similar reaction times speeds, with non-gamers/athletes having the slowest. However, we wanted to test the reaction times of all groups, based on them simply taping a button/mouse already …


The Effect Of Distance From Habitat Fragmentation On Biodiversity Of Invertebrates, Diego Sifuentes, Coleman Taylor Nov 2021

The Effect Of Distance From Habitat Fragmentation On Biodiversity Of Invertebrates, Diego Sifuentes, Coleman Taylor

Fall Showcase for Research and Creative Inquiry

Habitat fragmentation happens when human interaction causes an organism's environment to emerge in discontinuities in their habitat. This leads to degradation and disrupts the ecosystem as a whole. This experiment will try to answer the question if habitat fragmentation is still prevalent on invertebrates at a further range. This will be achieved by setting two sites with pitfall traps on both sides on the fragmentation and then a third site will be added at the same distance from the first two sites. It is hypothesized that there will be a less abundance of invertebrates on sites A and B while …


Masking Away Our Emotions, Marshall White, Kyle Hiatt Nov 2021

Masking Away Our Emotions, Marshall White, Kyle Hiatt

Fall Showcase for Research and Creative Inquiry

The use of face masks has the potential to greatly reduce the efficacy and ability of nonverbal communication during in-person social interactions. The purpose of this study is to determine the consequences and implications that face masks have on nonverbal communication, as well as the effect that the use of face masks has on an individual's ability to perceive emotions based on facial expressions. It is believed that an individual would be statistically successful matching an unmasked facial expression to a pictured emotion, but not as statistically successful matching a masked facial expression to a pictured emotion. This study will …


Comparing The Microbial Diversity Of Campus Cultivated Flowers Vs Eec Wildflowers At Longwood University, Elizabeth Whitehead, Rodney Clark, Quinn Kehoe Nov 2021

Comparing The Microbial Diversity Of Campus Cultivated Flowers Vs Eec Wildflowers At Longwood University, Elizabeth Whitehead, Rodney Clark, Quinn Kehoe

Fall Showcase for Research and Creative Inquiry

The purpose of this experiment is to see which type of bacteria are found on Longwood University's plant life. Identities of three of the flowers were found using genomics. It was found that there was a greater quantity of bacteria colonies on the wildflowers behind the EEC building however, there was greater diversity of bacterial colonies on the Rotunda flowers.


Micro-Rna, A Solution For Understanding Depression, Marshall White Nov 2021

Micro-Rna, A Solution For Understanding Depression, Marshall White

Fall Showcase for Research and Creative Inquiry

Major depressive disorder is a mood disorder that causes sadness and loss of interest. This affects how one may feel, think, or behave which impact their physical and mental state. People who have been diagnosed with this disorder find themselves losing interest in things they once loved. In the United States there are 16.1 million Americans diagnosed with MDD. This disorder is more common in individuals who have had it in their families which has resulted in the interest in how different genes impact MDD. Micro-RNA has been researched and discovered to have a correlation with MDD. These Micro-RNA's show …


The Process Of Isolating, Purifying, And Analyzing The Gfp Gene From The Pglo Plasmid, And Transforming And Expressing Gfp In E. Coli Cells., Kailyn Westbrook Nov 2021

The Process Of Isolating, Purifying, And Analyzing The Gfp Gene From The Pglo Plasmid, And Transforming And Expressing Gfp In E. Coli Cells., Kailyn Westbrook

Fall Showcase for Research and Creative Inquiry

The aim of this project was to isolate, amplify, and purify the GFP gene from the pGLO plasmid, for both analysis as well as the translation and expression into Escherichia coli cell cultures. The Polymerase Chain Reaction (PCR) technique was used to isolate and amplify the GFP gene, which was then purified for use in the agarose gel electrophoresis (AGE) chamber, to distinguish the DNA fragments by size and determine the presence of the desired amplicon. The NanoDrop, SnapGene, and Basic Local Alignment Search Tool (BLAST) softwares were then used for the analysis of the purified amplicon. During the analysis …


Using Freemocap Software To Examine And Quantify Bodily Movements, Delaney Mcmahon, Jeri Tyler Nov 2021

Using Freemocap Software To Examine And Quantify Bodily Movements, Delaney Mcmahon, Jeri Tyler

Fall Showcase for Research and Creative Inquiry

Human motion capture has been used to study skeletal biomechanics by analyzing bodily movements. The most common way to analyze body movements is to place reflective markers on the joints and record the movements to then move frame by frame to capture the desired joint angles. However new software has been developed called FreeMoCap in which this research examined. FreeMoCap easily quantified bodily movements and is easy to use by the common person. This software is easily downloaded onto a computer at home and only requires two cameras to use and does all analyzing of the movements automatically. Having a …


I Spi With My Wgs Eye: A Salmonella Outbreak, Sophie Kidd Nov 2021

I Spi With My Wgs Eye: A Salmonella Outbreak, Sophie Kidd

Fall Showcase for Research and Creative Inquiry

Salmonella is the leading microbial source of foodborne illnesses, making it an important organism for public health studies. By examining trends between Salmonella cases through whole genome sequencing (WGS) and genomic analysis of Salmonella pathogenicity, the occurrences of outbreaks can be prevented. The genetic differences between Salmonella bacteria isolated from the environment and those isolated from clinical cases were investigated by identifying Salmonella Pathogenicity Islands (SPI). Salmonella Pathogenicity Islands are genetic systems which encode the pathogenic mechanisms for interactions with the host. Given that SPIs provide a wide range of possible methods for host-pathogen interactions, there will be a difference …


The Effect Of Pepck If The The Transcription Fact Dfoxo Is Knocked Down, Hunter Croom, Diego Sifuentes, Amari Riddick, Samari Gibson Nov 2021

The Effect Of Pepck If The The Transcription Fact Dfoxo Is Knocked Down, Hunter Croom, Diego Sifuentes, Amari Riddick, Samari Gibson

Fall Showcase for Research and Creative Inquiry

Cell Biology has evolved throughout the years from Hooke describing a cell for the first time to CRISPR now allowing scientists to think about gene editing. This experiment we will be using one of these advancements to see the effect on PEPCK when dFOXO is knocked down. The gene; PEPCK stands for Phosphoenolpyruvate carboxykinase, this enzyme typically is involved and is a key enzyme for the liver and the kidney. The transcription factor that will be inhibited is called FOXO, where transcription factors are binded to the target gene and begin to affect the development and metabolism. The results we …


Longevity And Aging Mediated By Foxo And The Gene Methuselah, Amanda Palmer, Hailee Banks, Kylee Wright, Tristana Foster Nov 2021

Longevity And Aging Mediated By Foxo And The Gene Methuselah, Amanda Palmer, Hailee Banks, Kylee Wright, Tristana Foster

Fall Showcase for Research and Creative Inquiry

Forkhead box (FOXO) transcription factors have been shown to influence longevity and ageing in various species. FOXO has shown to be involved in the regulation of cancer through the ageing process. The gene methuselah in Drosophila melanogaster is involved in longevity due to its connection to FOXO transcription factors. This study aims to discover the effects of FOXO on the gene methuselah through gene knockout. D. melanogaster cells were infused with FOXO transcription factors to alter the DNA strand. The methuselah gene within this DNA was knocked out by inhibition of the FOXO transcription factors. Knockout of this gene should …