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Full-Text Articles in Life Sciences
Optimization Of In-Vitro Tissue Culture And Transformation System For Sweet Sorghum Cultivars Using Immature Inflorescence, Logayn Abushal
Optimization Of In-Vitro Tissue Culture And Transformation System For Sweet Sorghum Cultivars Using Immature Inflorescence, Logayn Abushal
Theses and Dissertations
Background: Sweet sorghum is a multi-purpose crop that can be used for human food, animal feed, and bio-fuel production. This crop is drought-tolerant and can therefore be grown in arid and semi-arid regions especially in marginal areas in Egypt. Nevertheless, production of Sweet sorghum cannot coup with the increasing demand in consumption as a food, and fuel due to a lag in scientific research regarding its genetic improvement. Therefore, genetic improvement of Sweet sorghum is innately required so that new traits of economic importance could be introduced; however, this can only be achieved through the establishment of a robust in-vitro …
Self-Extending Symbiosis In Symbiodinium Microadriaticum And The Effect Of Endosymbiotic / Horizontal Gene Transfer On Dinoflagellates And The Tree Of Life, Maha Kadry Ahmed Fathy
Self-Extending Symbiosis In Symbiodinium Microadriaticum And The Effect Of Endosymbiotic / Horizontal Gene Transfer On Dinoflagellates And The Tree Of Life, Maha Kadry Ahmed Fathy
Archived Theses and Dissertations
Symbiodinium microadriaticum is a dinoflagellate that lives in a mutualistic relationship with the coral Stylophora pistillata in the Red Sea. Symbiodinium microadriaticum is divided into several clades and the A1 clade of Symbiodinium microadriaticum lives in shallow waters and is resilient to thermal stress. The association between Symbiodinium microadriaticum A1 and Stylophora pistillata enables Stylophora pistillata to tolerate the rise in the sea surface temperature caused by global warming and climate change. Here, the first completely sequenced dinoflagellate genome is explored to answer two questions: The first question is whether self-extending symbiosis has a role in the ability of Symbiodinium …
Enrichment Of Extremophilic Bacteria From Atlantis Ii Deep Brine Sediments Using Various Electron Donors & Acceptors, Nelly Abu Alkheir
Enrichment Of Extremophilic Bacteria From Atlantis Ii Deep Brine Sediments Using Various Electron Donors & Acceptors, Nelly Abu Alkheir
Archived Theses and Dissertations
The Atlantis II Deep is the most studied and largest brine pool in the Red Sea. It is an active hydrothermal habitat with unique physical conditions. Its unique characteristics (70°C temperature, 26% salinity, pH 5.2) caused it to be a very attractive marine ecosystem for microbiological and metagenomics studies. Sediments of the Atlantis II Deep brine were investigated for the presence of extremophilic bacteria using a cultivation approach combined with phylogenetic analysis. Two different cultivation methods were utilized: substrate gradient cultures and minimal salts medium supplemented with various combinations of electron donors and acceptors. A bacterial culture enriched from the …
Metagenomic Assessment Of The Mobilomes Of Red Sea's Atlantis Ii, Discovery And Kebrit Deeps, Mohamed Mohamed El Rifai
Metagenomic Assessment Of The Mobilomes Of Red Sea's Atlantis Ii, Discovery And Kebrit Deeps, Mohamed Mohamed El Rifai
Archived Theses and Dissertations
Mobile genetic elements in microbial communities are mainly composed of plasmids, transposons and viruses. They are collectively referred to as the mobilome and serve as agents that facilitate horizontal gene transfer, which plays an important role in evolution and adaptation of microorganisms. In order to study the abundance and distribution of mobile genetic elements in different marine environments and to clarify whether certain environmental conditions could explain such distribution, sequence similarity searches using protein family signatures against diverse marine metagenomic datasets were used to determine the relative frequency of coding sequences associated with transposases, integrases, plasmids and viruses, or phages. …
E2 Evolutionary Motif Prediction And Identification Of Drug Candidates To Prevent Hepatitis C Virus Cellular Entry, Shairy Azmy Danial
E2 Evolutionary Motif Prediction And Identification Of Drug Candidates To Prevent Hepatitis C Virus Cellular Entry, Shairy Azmy Danial
Archived Theses and Dissertations
Hepatitis C Virus (HCV) infection causes around three quarters of worldwide chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Interferon and ribavirin combination therapy is not effective with all HCV genotypes, and has serious side effects that may result in discontinuation of treatment. The aim of our work is to design inhibitors against HCV envelope glycoprotein E2 to prevent its binding to CD81 surface receptor and the consequent viral entry into hepatocytes. Currently, no crystal structure is constructed for E2. However, a model of E2 is available, Yagnik et al. model, which served as the starting point for the structure-based inhibitor …
Exploring The Aerobic Methanotrophs Community In The Red Sea Atlantis Ii Deep Brine Area, Rehab Abdallah
Exploring The Aerobic Methanotrophs Community In The Red Sea Atlantis Ii Deep Brine Area, Rehab Abdallah
Archived Theses and Dissertations
Methane represents one of the most abundant hydrocarbons on earth and a powerful greenhouse gas. Methane oxidation by microbial consortia contributes positively to the environment by decreasing methane emission to the atmosphere from different ecosystems. Aerobic methanotrophic bacteria are considered one of the main groups involved in methane oxidation. Brine pools are dynamic habitats for microbial cycling of carbon. Atlantis II Deep is the largest brine pool in the Red Sea. The pool is well known for its elevated temperature, salinity and methane concentration. Methane seeps from the deep brine to the overlying brine seawater interface layer, providing a suitable …
Unique Biodiversity Of The Red Sea Brine Floor Revealed By Comparative Taxonomic Analysis Of Atlantis Ii And Discovery Deep Brine Sediments, Ghada Alaa El-Din Mustafa
Unique Biodiversity Of The Red Sea Brine Floor Revealed By Comparative Taxonomic Analysis Of Atlantis Ii And Discovery Deep Brine Sediments, Ghada Alaa El-Din Mustafa
Archived Theses and Dissertations
The sea floor is a unique and diverse ecosystem to investigate how geochemical processes affect the diversity of biological life. Red Sea brine pools are water bodies characterized by a unique multitude of extreme conditions, including high temperature, high salinity, and unusual high concentration of heavy metals. We performed a comparative metagenomic analysis of the microbial communities in the sea floor of the Atlantis II (2168 m; 68oC), Discovery Deep (2166 m; 48oC) brine pools and two non-brinesites (1,856 m; 21.93°C and 1,937 m; 31.87°C) in the Red Sea. Each brine core wasvertically dissected into seven distinct subsections. CHN&S profiles …
Bacterial Production Of Human Discoidin Domain Receptor 2 (Ddr2) Kinase And Its Gatekeeper Mutants To Assess Activation By Phosphorylation, Dina Allam Abdel Azeem
Bacterial Production Of Human Discoidin Domain Receptor 2 (Ddr2) Kinase And Its Gatekeeper Mutants To Assess Activation By Phosphorylation, Dina Allam Abdel Azeem
Archived Theses and Dissertations
Discoidin domain receptor 2 (DDR2) belongs to a subfamily of receptor tyrosine kinases (RTKs) known as discoidin domain receptors (DDRs). DDRs are characterized by an extracellular discoidin homology domain and being activated by collagen resulting in a delayed but sustained activation. DDR2 overexpression was reported in some tumors, atherosclerosis as well as a number of inflammatory and fibrotic conditions. Recently 3 kinase inhibitor drugs (imatinib, nilotinib and dasatinib) were reported to inhibit DDR2 kinase activity suggesting it might have a role in their therapeutic effect. However minimal information is available on DDR2 downstream signaling. Identification of DDR2 substrates is essential …
Molecular Characterization Of Vibrio Cholerae Isolates From African Countries, Lamyaa S. Abdel Hamid
Molecular Characterization Of Vibrio Cholerae Isolates From African Countries, Lamyaa S. Abdel Hamid
Archived Theses and Dissertations
Vibrio cholerae has caused seven global pandemics to date. The ongoing pandemic, caused by Vibrio cholerae serogroup O1 El Tor, has been the most extensive in geographical spread and duration. It began in 1961 in Indonesia spreading through Asia and the Middle East and finally reaching Sub Saharan West Africa at the early 1970s. In the first year of the pandemic alone, over 150,000 cases and more than 20,000 deaths were recorded. V. cholerae is currently responsible for several outbreaks in Africa, some with a reported high mortality rate. Although extensive characterization has been carried out on epidemic strains in …
Isolation And Characterization Of A Novel Thermostable, Halotolerant L-Asparaginase Ii From The Metagenome Of The Red Sea; Atlantis Ii Brine Pool, Aya Medhat Saleh
Isolation And Characterization Of A Novel Thermostable, Halotolerant L-Asparaginase Ii From The Metagenome Of The Red Sea; Atlantis Ii Brine Pool, Aya Medhat Saleh
Archived Theses and Dissertations
L-Asparaginases (E.C. 3.5.1.1) are group of enzymes which catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Many microorganisms are known to produce two types of L-asparaginase. Considerable attention has been specifically drawn to type II L-asparaginases due to their potent tumor-inhibitory activity. Various limitations, such as high toxicity, poor stability and short half-life, are associated with the commercially available L-asparaginases used in cancer therapy. Additionally, L-asparaginases have a significant role in food industry owing to their ability to reduce the levels of acrylamide, which is a known neurotoxin and a potential carcinogen, in various food products. Knowing that …
Crispr Diversity In Brine Pools Of The Red Sea: A Comparative Study Of Clustered Regularly Interspaced Short Palindromic Repeats From The Red Sea Metagenomes, Mohamed Mohamed Lotaief
Crispr Diversity In Brine Pools Of The Red Sea: A Comparative Study Of Clustered Regularly Interspaced Short Palindromic Repeats From The Red Sea Metagenomes, Mohamed Mohamed Lotaief
Archived Theses and Dissertations
Exploration of CRISPR/Cas systems in the unique Red sea Brine pools can provide insights into host-viral interactions in highly extremophile environments. 454 Metagenomic datasets from different strata of Atlantis II, Discovery deep and Kebrit deep brine pools were mined for CRISPR arrays, and Cas proteins and compared to one another. Overall composition of CRISPR types was inferred by Cas protein HMM data, together with Cas1 phylogenetic placement using RaxML and direct repeat typing. Spacerâ viral interaction was explored using local alignments of mined spacer data to local metagenomic prophage sequences, showing and comparing specific sample CRISPR affected viral occurrences as …
Mining And Identification Of A Potential Subtilisin-Like Serine Protease From The Lower Convective Layer Of The Atlantis Ii Brine Pool In The Red Sea, Ahmed Shibl
Archived Theses and Dissertations
The Red Sea harbors a number of deep-sea brine pools with diverse chemical, physical, and geological conditions. One of the environments, the Atlantis II Brine Pool, is unique to the Red Sea. The deepest part of Atlantis II Brine Pool â the Lower Convective Layer (LCL) â is 2060 meters below the sea surface water, with 26% salinity, and a temperature of about 68 °C. Moreover, it is almost anoxic, and contains a very high concentration of heavy metals. We are interested in identifying subtilisin-like serine proteases, which are an important group of proteolytic enzymes with a broad range of …
Potential Endoglucanase Genes From The Red Sea Atlantis Ii Brine Pool: Structure, Organization And Halophilic Signature, Mohamad Maged Galal
Potential Endoglucanase Genes From The Red Sea Atlantis Ii Brine Pool: Structure, Organization And Halophilic Signature, Mohamad Maged Galal
Archived Theses and Dissertations
Cellulolytic enzymes have many industrial applications and play a major role in the process of biofuel production. Bioethanol is a cheap and clean alternative to non-renewable, polluting and expensive fossil fuel. Cellulases and hemicellulases act synergistically to degrade the cellulose polymer into glucose which can then be fermented by specific anaerobic organisms into ethanol. The overall manufacturing process occurs under harsh conditions such as low pH, high temperature and in the presence of elevated salt concentrations. The Atlantis II brine pool in the Red Sea is a unique habitat in terms of its diverse microbial community and physical conditions; high …
Triple Trouble: Examining The Effect Of Combining Mechanical Stress, Pbmcs And Cisplatin On Hepg2 Cell Death, Lalia Ziko
Triple Trouble: Examining The Effect Of Combining Mechanical Stress, Pbmcs And Cisplatin On Hepg2 Cell Death, Lalia Ziko
Archived Theses and Dissertations
In an attempt to find better cancer treatments, a new mathematical model proposed by Klika & Marsik (2009) suggests that more cancer cells could possibly be killed by applying several stress factors to tumour cells, however only as a simulation in silico. The proposed stresses include healthy cell anti-tumour effect, the biochemical effect exerted by a cytotoxic drug and a newly introduced mechanical stress. The hypothesis entails that upon applying these three stress factors together simultaneously to tumour cells, the concentration of the tumour cells decreases more than if a stress factor was applied alone. The two main objectives in …
Identification Of Potential Subtilisin-Like Serine Proteases From The Microbial Community Of The Red Sea Atlantis-Ii Brine Pool Using A Metagenomic Approach, Nadine Hosny El-Said
Identification Of Potential Subtilisin-Like Serine Proteases From The Microbial Community Of The Red Sea Atlantis-Ii Brine Pool Using A Metagenomic Approach, Nadine Hosny El-Said
Archived Theses and Dissertations
The Atlantis II deep is the largest brine pool in the Red Sea of around 60 km2. It is located in the central Red Sea (21° 21' N., 38° 04' E.) at a depth of 2200 meters from the sea surface. The lower part of this pool, the lower convective layer (LCL), has extremely harsh environmental conditions characterized by a temperature of up to 68°C, pH 5.3, saturated salt (252 psu), anoxic condition, and high concentration of heavy metals. These harsh abiotic factors exert strong selective pressure in favor of microbial communities adapted to survive in such environment. Therefore, proteins …
Molecular Characterization Of Neuraminidase Surface Proteins Of Influenzavirus B Isolates From Egypt, 1999-2008, Nadean Safwan Khedr
Molecular Characterization Of Neuraminidase Surface Proteins Of Influenzavirus B Isolates From Egypt, 1999-2008, Nadean Safwan Khedr
Archived Theses and Dissertations
No abstract provided.
Deciphering The Unique Microrna Signature In Human Esophageal Adenocarcinoma: Dissecting Esophageal Adenocarcinoma Progressive Profile And Its Distinctions From Gastric Cancer, Rama Ahmed Saad Ahmed Ali
Deciphering The Unique Microrna Signature In Human Esophageal Adenocarcinoma: Dissecting Esophageal Adenocarcinoma Progressive Profile And Its Distinctions From Gastric Cancer, Rama Ahmed Saad Ahmed Ali
Archived Theses and Dissertations
No abstract provided.