Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Development Of Real-Time Pcr Assays For Rapid Detection Of Pfiesteria Piscicida And Related Dinoflagellates, Torstein Tengs
Development Of Real-Time Pcr Assays For Rapid Detection Of Pfiesteria Piscicida And Related Dinoflagellates, Torstein Tengs
Dr. Torstein Tengs
Pfiesteria complex species are heterotrophic and mixotrophic dinoflagellates that have been recognized as harmful algal bloom species associated with adverse fish and human health effects along the East Coast of North America, particularly in its largest (Chesapeake Bay in Maryland) and second largest (Albermarle- Pamlico Sound in North Carolina) estuaries. In response to impacts on human health and the economy, monitoring programs to detect the organism have been implemented in affected areas. However, until recently, specific identification of the two toxic species known thus far, Pfiesteria piscicida and P. shumwayae (sp. nov.), required scanning electron microscopy (SEM). SEM is a …
Phylogenetic Analyses Indicate That The 19'Hexanoyloxy-Fucoxanthin-Containing Dinoflagellates Have Tertiary Plastids Of Haptophyte Origin, Torstein Tengs
Phylogenetic Analyses Indicate That The 19'Hexanoyloxy-Fucoxanthin-Containing Dinoflagellates Have Tertiary Plastids Of Haptophyte Origin, Torstein Tengs
Dr. Torstein Tengs
The three anomalously pigmented dinoflagellates Gymnodinium galatheanum, Gyrodinium aureolum, and Gymnodinium breve have plastids possessing 199-hexanoyloxy-fucoxanthin as the major carotenoid rather than peridinin, which is characteristic of the majority of the dinoflagellates. Analyses of SSU rDNA from the plastid and the nuclear genome of these dinoflagellate species indicate that they have acquired their plastids via endosymbiosis of a haptophyte. The dinoflagellate plastid sequences appear to have undergone rapid sequence evolution, and there is considerable divergence between the three species. However, distance, parsimony, and maximum-likelihood phylogenetic analyses of plastid SSU rRNA gene sequences place the three species within the haptophyte clade. …
Heteroduplex Mobility Assay-Guided Sequence Discovery: Elucidation Of The Small Subunit (18s) Rdna Sequences Of Pfiesteria Piscicida And Related Dinoflagellates From Complex Algal Culture And Environmental Sample Dna Pools, Torstein Tengs
Dr. Torstein Tengs
The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, and with a novel clinical syndrome of impaired cognition and memory disturbance among humans after presumptive toxin exposure. As a result, there is a pressing need to better characterize the organism and these associations. Advances in Pfiesteria research have been hampered, however, by the absence of genomic sequence data. We employed a sequencing strategy directed by heteroduplex mobility assay to detect Pfiesteria piscicida 18S rDNA ‘‘signature’’ sequences in complex pools of DNA and used those data as the basis for determination …