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Full-Text Articles in Life Sciences
A New Versatile System For Freeze-Substitution, Freeze-Drying And Low Temperature Embedding Of Biological Specimens, H. Sitte, L. Edelmann, H. Hässig, H. Kleber, A. Lang
A New Versatile System For Freeze-Substitution, Freeze-Drying And Low Temperature Embedding Of Biological Specimens, H. Sitte, L. Edelmann, H. Hässig, H. Kleber, A. Lang
Scanning Microscopy
A universal system for freeze-substitution (FS), freeze-drying (FD) and low temperature embedding (LTE) has been developed, suited to perform standardized procedures of cryoprocessing biological and medical specimens as well as systematic studies of dehydration and embedding at various low and high temperatures. In a 35 I Dewar vessel with 110 mm neck diameter an aluminum tube is mounted to the bottom of the liquid nitrogen (LN2x) reservoir and extends to the lower part of the cylindrical neck. At its top an aluminum plate serves as a contact surface for either the FS chamber or the FD chamber. FS …
Optimal Freeze-Drying Of Cryosections And Bulk Specimens For X-Ray Microanalysis, L. Edelmann
Optimal Freeze-Drying Of Cryosections And Bulk Specimens For X-Ray Microanalysis, L. Edelmann
Scanning Microscopy
Electron microscopic investigations of rapidly frozen specimens of striated muscle, either frozen-hydrated or obtained after different dehydration procedures, have shown that the subcellular distribution of the main cellular cation K+ or its surrogates Rb+, Cs+, or Tl+ does not follow the water distribution but follows certain proteins. Conflicting results obtained by X-ray microanalysis of freeze-dried cryosections are explained by showing that freeze-drying of bulk specimens and cryosections must be carried out for rather long periods at low temperature in order to avoid severe shrinkage and ion redistribution artefacts. Proposals for future freeze-drying studies are …
Low Temperature Embedding Of Chemically Unfixed Biological Material After Cryosorption Freeze-Drying, L. Edelmann
Low Temperature Embedding Of Chemically Unfixed Biological Material After Cryosorption Freeze-Drying, L. Edelmann
Scanning Microscopy
After freeze-drying of bulk specimens in a newly developed cryosorption freeze-dryer (CFD) a special accessory is used to infiltrate the specimens in Lowicryl HM20 and to polymerize them at low temperature by ultra-violet (UV) irradiation within the CFD chamber in flat embedding moulds. The accessory allows polymerization in a dry, oxygen free environment without the risk of evaporation of volatile components of the resin which may lead to unsatisfactory polymerization. First results demonstrate the quality of structure preservation of biological material not treated with any chemical fixative.