Life Sciences Commons^™

Simulation With Pharmacological Agents Of Radiation Damage To Small Intestinal Villi, M. Indran, F. C. Boyle, K. E. Carr

Scanning Electron Microscopy

Irradiation induces damage to intestinal villi, resulting in a progressive decline in villous height and changes in topography. Gamma and neutron radiation are reported to cause changes in the structure of smooth muscle and nerve twigs of the intestinal wall. It is possible, therefore, that villous collapse may be due partly to changes in the underlying stromal elements as a result of damage to nerve or muscle.

To test this hypothesis, mice were treated with the drug reserpine which is known to affect the neural control of intestinal smooth muscle function and the small intestine was examined for topographical and …

Structural Changes In Mouse Small Intestinal Villi Following Lower Body Hyperthermia, H. M. H. Kamel, K. E. Carr, S. P. Hume, J. C. L. Marigold

Scanning Electron Microscopy

Heating an exteriorised loop of mouse small intestine resulted in marked changes in the shape of the villi as reported earlier. However, the exteriorisation techniques resulted in non-uniformity in both temperature and effect around circumference of intestine and, in addition, the extent to which handling contributed to the observed damage was not known. The work has therefore been extended using lower-body heating in the temperature range 37.5° - 43.0°C.

Heating in the temperature range 37.5° to 41.0°C produced minimal to moderate structural changes, manifested as scattered, vertically collapsed villi amongst predominantly "normal" villi. No villi showed conical or rudimentary forms …

In Vivo Light Microscopy Of Organs, Robert S. Mccuskey

Scanning Electron Microscopy

High resolution, brightfield and fluorescence, light microscopic methods have been developed for examining living organs in situ. The methods permit study of the rate, duration, magnitude and direction of dynamic histologic, pathologic, physiologic and pharmacologic events. In addition, morphometric analyses of such living preparations can provide basic information needed to evaluate alterations induced by fixation and processing of these organs for electron microscopy. Most organs are amenable to such investigations. In anesthetized animals, the selected organ is trans-and/or epi-illuminated with selected wavelengths of monochromatic light, imaged with water immersion objectives and the resulting monochromatic optical images televised using silicon …