Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
The Electron Microscope Detection And X-Ray Quantitation Of Cations In Bacterial Cells, D. C. Sigee, M. H. El-Masry, R. H. Al-Rabaee
The Electron Microscope Detection And X-Ray Quantitation Of Cations In Bacterial Cells, D. C. Sigee, M. H. El-Masry, R. H. Al-Rabaee
Scanning Electron Microscopy
Electron microscope autoradiography and X-ray microanalysis have been used for the detection and quantitation of cations in the bacterium Pseudomonas tabaci. These techniques differ in the information they provide (relating to either cation uptake or in situ levels), their applicability to different cations, their sensitivity and their spatial resolution. With uptake of 63Ni2+, high resolution autoradiography (involving gold latensification and physical development) demonstrated a high degree of cation localisation to the central nucleoid area (glutaraldehyde-fixed cells) and within this to the constituent chromatin (acetic-alcohol preparations).
X-ray microanalysis of whole bacterial cells revealed the presence of substantial levels …