Life Sciences Commons^™

Global Patterns And Predictions Of Seafloor Biomass Using Random Forests, Chih-Lin Wei, Gilbert T. Rowe, Elva Escobar-Briones, Antje Boetius, Thomas Soltwedel, M. Julian Caley, Yousria Soliman, Falk Huettmann, Fangyuan Qu, Zishan Yu, C. Roland Pitcher, Richard L. Haedrich, Mary K. Wicksten, Michael A. Rex, Jeffrey G. Baguley, Jyotsna Sharma, Roberto Danovaro, Ian R. Macdonald, Clifton C. Nunnally, Jody W. Deming, Paul Montagna, Mélanie Lévesque, Jan Marcin Weslawski, Maria Wlodarska-Kowalczuk, Baban S. Ingole, Brian J. Bett, David S. M. Billett, Andrew Yool, Bodil A. Bluhm, Katrin Iken, Bhavani E. Narayanaswamy

Biology Faculty Publication Series

A comprehensive seafloor biomass and abundance database has been constructed from 24 oceanographic institutions worldwide within the Census of Marine Life (CoML) field projects. The machine-learning algorithm, Random Forests, was employed to model and predict seafloor standing stocks from surface primary production, water-column integrated and export particulate organic matter (POM), seafloor relief, and bottom water properties. The predictive models explain 63% to 88% of stock variance among the major size groups. Individual and composite maps of predicted global seafloor biomass and abundance are generated for bacteria, meiofauna, macrofauna, and megafauna (invertebrates and fishes). Patterns of benthic standing stocks were positive …

Spatial Distribution Of Calcium-Gated Chloride Channels In Olfactory Cilia, Donald A. French, Dorjsuren Badamdorj, Steven J. Kleene

Mathematical Sciences Faculty Research

Background

In vertebrate olfactory receptor neurons, sensory cilia transduce odor stimuli into changes in neuronal membrane potential. The voltage changes are primarily caused by the sequential openings of two types of channel: a cyclic-nucleotide-gated (CNG) cationic channel and a calcium-gated chloride channel. In frog, the cilia are 25 to 200 µm in length, so the spatial distributions of the channels may be an important determinant of odor sensitivity.

Principal Findings

To determine the spatial distribution of the chloride channels, we recorded from single cilia as calcium was allowed to diffuse down the length of the cilium and activate the channels. …

Conclusions About Niche Expansion In Introduced Impatiens Walleriana Populations Depend On Method Of Analysis, Lisa Mandle, Dan L. Warren, Matthias H. Hoffman, A. Townsend Peterson, Johanna Schmitt, Eric J. Von Wettberg

Department of Biological Sciences

Determining the degree to which climate niches are conserved across plant species’ native and introduced ranges is valuable to developing successful strategies to limit the introduction and spread of invasive plants, and also has important ecological and evolutionary implications. Here, we test whether climate niches differ between native and introduced populations of Impatiens walleriana, globally one of the most popular horticultural species. We use approaches based on both raw climate data associated with occurrence points and ecological niche models (ENMs) developed with Maxent. We include comparisons of climate niche breadth in both geographic and environmental spaces, taking into account differences …

Biphasic Targeting And Cleavage Furrow Ingression Directed By The Tail Of A Myosin Ii, X. Fang, J. Y. Luo, R. Nishihama, C. Wloka, C. Dravis, M. Travaglia, M. Iwase, Elizabeth Ann Vallen, E. Bi

Biology Faculty Works

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with membrane trafficking during cytokinesis, remains poorly understood. Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its tail that act sequentially during the cell cycle. Before cytokinesis, Myo1 localization depends on the septin-binding protein Bni5. During cytokinesis, Myo1 localization depends on the IQGAP Iqg1. We also show that the Myo1 …

Complexity Through Recombination: From Chemistry To Biology, Niles Lehman, Carolina Diaz Arenas, Wesley A. White, Francis J. Schmidt

Chemistry Faculty Publications and Presentations

Recombination is a common event in nature, with examples in physics, chemistry, and biology. This process is characterized by the spontaneous reorganization of structural units to form new entities. Upon reorganization, the complexity of the overall system can change. In particular the components of the system can now experience a new response to externally applied selection criteria, such that the evolutionary trajectory of the system is altered. In this work we explore the link between chemical and biological forms of recombination. We estimate how the net system complexity changes, through analysis of RNA-RNA recombination and by mathematical modeling. Our results …

Role For Sumoylation In Systemic Inflammation And Immune Homeostasis In Drosophila Larvae, Indira Paddibhatla, Mark J. Lee, Marta E. Kalamarz, Roberto Ferrarese, Shubha Govind

Publications and Research

To counter systemic risk of infection by parasitic wasps, Drosophila larvae activate humoral immunity in the fat body and mount a robust cellular response resulting in encapsulation of the wasp egg. Innate immune reactions are tightly regulated and are resolved within hours. To understand the mechanisms underlying activation and resolution of the egg encapsulation response and examine if failure of the latter develops into systemic inflammatory disease, we correlated parasitic wasp-induced changes in the Drosophila larva with systemic chronic conditions in sumoylation-deficient mutants. We have previously reported that loss of either Cactus, the Drosophila (IkB) protein or Ubc9, the SUMO-conjugating …

10th Annual Senior Research Symposium Of The Department Of Biological Sciences, Chemistry And Biochemistry, Messiah College

School of Science, Engineering & Health (SEH) Symposium

No abstract provided.

Adaptive Divergence In The Thyroid Hormone Signaling Pathway In The Stickleback Radiation, Jun Kitano, Sean C. Lema, J. Adam Luckenbach, Seiichi Mori, Yui Kawagishi, Makoto Kusakabe, Penny Swanson, Catherine L. Peichel

Biological Sciences

During adaptive radiations, animals colonize diverse environments, which requires adaptation in multiple phenotypic traits. Because hormones mediate the dynamic regulation of suites of phenotypic traits, evolutionary changes in hormonal signaling pathways might contribute to adaptation to new environments. Here we report changes in the thyroid hormone signaling pathway in stream-resident ecotypes of threespine stickleback fish (Gasterosteus aculeatus), which have repeatedly evolved from ancestral marine ecotypes. Stream-resident fish exhibit a lower plasma concentration of thyroid hormone and a lower metabolic rate, which is likely adaptive for permanent residency in small streams. The thyroid-stimulating hormone-β2 (TSHβ …

Type Ii Protein Arginine Methyltransferase 5 (Prmt5) Is Required For Circadian Pperiod Determination In Arabidopsis Thaliana, Sunghyun Hong, Hae-Ryoung Song, Kerry Lutz, Randall A. Kerstetter, Todd P. Michael, C. Robertson Mcclung

Dartmouth Scholarship

Posttranslational modification is an important element in circadian clock function from cyanobacteria through plants and mammals. For example, a number of key clock components are phosphorylated and thereby marked for subsequent ubiquitination and degradation. Through forward genetic analysis we demonstrate that protein arginine methyltransferase 5 (PRMT5; At4g31120) is a critical determinant of circadian period in Arabidopsis. PRMT5 is coregulated with a set of 1,253 genes that shows alterations in phase of expression in response to entrainment to thermocycles versus photocycles in constant temperature. PRMT5 encodes a type II protein arginine methyltransferase that catalyzes the symmetric dimethylation of arginine residues (Rsme2). …

Campylobacter Ureolyticus: An Emerging Gastrointestinal Pathogen?, Susan Bullman, Daniel Corcoran, James O'Leary, Brigid Lucey, Deirdre Byrne, Roy D. Sleator

Department of Biological Sciences Publications

A total of 7194 faecal samples collected over a 1-year period from patients presenting with diarrhoea were screened for Campylobacter spp. using EntericBios, a multiplex-PCR system. Of 349 Campylobacter-positive samples, 23.8% were shown to be Campylobacter ureolyticus, using a combination of 16S rRNA gene analysis and highly specific primers targeting the HSP60 gene of this organism. This is, to the best of our knowledge, the first report of C. ureolyticus in the faeces of patients presenting with gastroenteritis and may suggest a role for this organism as an emerging enteric pathogen.

Flank Gland-Secreted Putative Chemosignals Pertaining To Photoperiod, Endocrine States, And Sociosexual Behavior In Golden Hamsters, Ying-Juan Liu, Da-Wei Wang, Lixing Sun, Jin-Hua Zhang, Jian-Xu Zhang

All Faculty Scholarship for the College of the Sciences

Behavioral studies have shown that flank glands are involved in chemical communication in golden hamsters Mesocricetus auratus but little chemical analysis has been conducted on volatiles arising from these glands. Using gas chromatography- mass spectrometry, we detected compounds from the flank glands of males, only eight of which were also produced in females. Based on these chemical data we performed a number of further experiments. By manipulating light we found that males exposed to short-photoperiods developed smaller flank glands than those exposed to long-photoperiods. Six flank gland volatiles reduced in relative abundance, which possibly coded for reproductive status of males …

Flowering Locus C Influences The Timing Of Shoot Maturation In Arabidopsis Thaliana, Laurie Mentzer, Tarah Yee, Tina Y. Wang, Edward Himelblau

Biological Sciences

No abstract provided.

Population Structure And Conservation Genetics Of The Oregon Spotted Frog, Rana Pretiosa, Michael S. Blouin, Ivan C. Phillipsen, Kirsten Monsen

Department of Biology Faculty Scholarship and Creative Works

The Oregon spotted frog (Rana pretiosa) is one of the most threatened amphibians in the Pacific Northwest. Here we analyzed data from 13 microsatellite loci and 298 bp of mitochondrial DNA in frogs collected from 23 of the remaining R. pretiosa populations in order to (1) assess levels of genetic diversity within populations of R. pretiosa, (2) identify the major genetic groups in the species, (3) estimate levels of genetic differentiation and gene flow among populations within each major group, and (4) compare the pattern of differentiation among R. pretiosa populations with that among populations of R. cascadae, a non-endangered …

The Gel Documentation System: A Cornerstone To The Implementation Of The Introduction To Biotechnology And Introduction To Bioinformatics Cross-Disciplinary Course Series (Final Report), Marcy Kelly, Gregory Lampard, Constance Knapp

Cornerstone 3 Reports : Interdisciplinary Informatics

Our original goal was to offer Pace undergraduate students opportunities to be introduced to both Biotechnology and Computer Science as it relates to Bioinformatics. We proposed a two course series, offered to both computer science and biology students that will increase both biological and computer science literacy of our students. The two courses are Introduction to Biotechnology (BIO 372) and Introduction to Bioinformatics.

Tracing Water Sources Of Terrestrial Animal Populations With Stable Isotopes: Laboratory Tests With Crickets And Spiders, Kevin E. Mccluney, John L. Sabo

Biological Sciences Faculty Publications

Fluxes of carbon, nitrogen, and water between ecosystem components and organisms have great impacts across levels of biological organization. Although much progress has been made in tracing carbon and nitrogen, difficulty remains in tracing water sources from the ecosystem to animals and among animals (the ‘‘water web’’). Naturally occurring, nonradioactive isotopes of hydrogen and oxygen in water provide a potential method for tracing water sources. However, using this approach for terrestrial animals is complicated by a change in water isotopes within the body due to differences in activity of heavy and light isotopes during cuticular and transpiratory water losses. Here …

The Burden Of Motherhood: The Effect Of Reproductive Load On Female Lizard Locomotor, Foraging, And Social Behavior, Michele A. Johnson, J. L. Caton, R. E. Cohen, J. R. Vandecar, J. Wade

Biology Faculty Research

The costs of reproduction, involving demands associated with both current and future reproductive efforts, may place a substantial burden on females. However, animals may minimize these costs by modifying their behavior across the reproductive cycle. We examined the effects of reproductive load on three types of behavior (locomotion, foraging, and social displays) in green anole lizards (Anolis carolinensis) by comparing egg, follicle, and oviduct mass and volume with field observational data. We found that female locomotor and social display behaviors decreased as reproductive load increased, suggesting behavioral modification in these traits, but we detected no relationship between foraging and …

Ecology And Transmission Of Buruli Ulcer Disease: A Systematic Review, Richard W. Merritt, Edward D. Walker, Pamela L. C. Small, John R. Wallace, Paul D. R. Johnson, Mark Eric Benbow, Daniel A. Boakye

Biology Faculty Publications

Buruli ulcer is a neglected emerging disease that has recently been reported in some countries as the second most frequent mycobacterial disease in humans after tuberculosis. Cases have been reported from at least 32 countries in Africa (mainly west), Australia, Southeast Asia, China, Central and South America, and the Western Pacific. Large lesions often result in scarring, contractual deformities, amputations, and disabilities, and in Africa, most cases of the disease occur in children between the ages of 4–15 years. This environmental mycobacterium, Mycobacterium ulcerans, is found in communities associated with rivers, swamps, wetlands, and human-linked changes in the aquatic environment, …

Identification Of Functional Elements And Regulatory Circuits By Drosophila Modencode, Sushmita Roy, Jason Ernst, Peter Kharchenko, Pouya Kheradpour, Nicolas Negre, Matthew Eaton, Jane Landolin, Christopher Bristow, Lijia Ma, Michael Lin, Stefan Washietl, Bradley Arshinoff, Ferhat Ay, Patrick Meyer, Nicolas Robine, Nicole Washington, Luisa Di Stefano, Eugene Berezikov, Christopher Brown, Rogerio Candeias, Joseph Carlson, Adrian Carr, Irwin Jungreis, Daniel Marbach, Rachel Sealfon, Michael Tolstorukov, Sebastian Will, Artyom Alekseyenko, Carlo Artieri, Benjamin Booth, Angela Brooks, Qi Dai, Carrie Davis, Michael Duff, Xin Feng, Andrey Gorchakov, Tingting Gu, Jorja Henikoff, Philipp Kapranov, Renhua Li, Heather Macalpine, John Malone, Aki Minoda, Jared Nordman, Katsutomo Okamura, Marc Perry, Sara Powell, Nicole Riddle, Akiko Sakai, Anastasia Samsonova, Jeremy Sandler, Yuri Schwartz, Noa Sher, Rebecca Spokony, David Sturgill, Marijke Van Baren, Kenneth Wan, Li Yang, Charles Yu, Elise Feingold, Peter Good, Mark Guyer, Rebecca Lowdon, Kami Ahmad, Justen Andrews, Bonnie Berger, Steven Brenner, Michael R. Brent, Lucy Cherbas, Sarah C.R. Elgin, Thomas Gingeras, Robert Grossman, Roger Hoskins, Thomas Kaufman, William Kent, Mitzi Kuroda, Terry Orr-Weaver, Norbert Perrimon, Vincenzo Pirrotta, James Posakony, Bing Ren, Steven Russell, Peter Cherbas, Brenton Graveley, Suzanna Lewis, Gos Micklem, Brian Oliver, Peter Park, Susan Celniker, Steven Henikoff, Gary Karpen, Eric Lai, David Macalpine, Lincoln Stein, Kevin White, Manolis Kellis

Biology Faculty Publications & Presentations

To gain insight into how genomic information is translated into cellular and developmental programs, the Drosophila model organism Encyclopedia of DNA Elements (modENCODE) project is comprehensively mapping transcripts, histone modifications, chromosomal proteins, transcription factors, replication proteins and intermediates, and nucleosome properties across a developmental time course and in multiple cell lines. We have generated more than 700 data sets and discovered protein-coding, noncoding, RNA regulatory, replication, and chromatin elements, more than tripling the annotated portion of the Drosophila genome. Correlated activity patterns of these elements reveal a functional regulatory network, which predicts putative new functions for genes, reveals stage- and …

Counterintuitive Effects Of Large-Scale Predator Removal On A Midlatitude Rodent Community, John L. Maron, Dean E. Pearson, Robert J. Fletcher Jr.

Biological Sciences Faculty Publications

Historically, small mammals have been focal organisms for studying predator–prey dynamics, principally because of interest in explaining the drivers of the cyclical dynamics exhibited by northern vole, lemming, and hare populations. However, many small-mammal species occur at relatively low and fairly stable densities at temperate latitudes, and our understanding of how complex predator assemblages influence the abundance and dynamics of these species is surprisingly limited. In an intact grassland ecosystem in western Montana, USA, we examined the abundance and dynamics of Columbian ground squirrels (Spermophilus columbianus), deer mice (Peromyscus maniculatus), and montane voles (Microtus montanus …

What Brown Saw And You Can Too, Philip Pearle, Brian Collett, Kenneth Bart, David Bilderback, Dara Newman, D. Scott Samuels

Biological Sciences Faculty Publications

A discussion of Robert Brown’s original observations of particles ejected by pollen of the plant Clarkia pulchella undergoing what is now called Brownian motion is given. We consider the nature of those particles and how he misinterpreted the Airy disk of the smallest particles to be universal organic building blocks. Relevant qualitative and quantitative investigations with a modern microscope and with a “homemade” single lens microscope similar to Brown’s are presented.

Science As A Human Right: Esa And The Aaas Science And Human Rights Coalition, Clifford Duke, George Middendorf, Jessica Wyndham

Department of Biology Faculty Publications

No abstract provided.

Microsatellite Development, Population Structure And Demographic Histories For Two Species Of Amazonian Peacock Bass Cichla Temensis And Cichla Monoculus (Perciformes: Cichlidae)., Jason C. Macrander

School of Biological Sciences: Dissertations, Theses, and Student Research

The Neotropics of South America represent one of the most diverse assemblages of freshwater organisms in the world. The geologic and ecological changes that have occurred throughout the Amazon and Orinoco River basins have resulted in the two most diverse rivers in the world producing very heterogeneous environments potentially influencing current population structure of Neotropical species. To investigate specific ecological and geological events influencing populations in these regions, I explore population structure and demographic histories of two species from the genus Cichla with wide distributions among the Amazon and Orinoco. First, I describe the methods used to isolate and characterize …

Eighteen Microsatellite Loci Developed From Western Burrowing Owls (Athene Cunicularia Hypugaea), Brant C. Faircloth, Alexandra Title, Kevin Tan, Justin Welty, James R. Belthoff, Patricia Adair Gowaty

Biology Faculty Publications and Presentations

Western burrowing owls (Athene cunicularia hypugaea) are ground-dwelling owls distributed throughout western North America. Because of population declines, this species is considered endangered in Canada, and burrowing owls are listed as a species of conservation concern in states of the western USA. Korfanta et al. (2002) previously presented primers for seven microsatellite loci in burrowing owls. Parentage and relatedness studies require a larger number of markers for accuracy and precision. Here, we developed and characterized 18 additional microsatellite DNA loci, and we tested these loci in 23 individuals. The number of alleles per locus ranged from 2 to …

[Accepted Article Manuscript Version (Postprint)] Pex5 Mutants That Differentially Disrupt Pts1 And Pts2 Peroxisomal Matrix Protein Import In Arabidopsis, Bibi Khan, Bethany Zolman

Biology Department Faculty Works

PEX5 and PEX7 are receptors required for the import of peroxisome-bound proteins containing one of two peroxisomal targeting signals (PTS1 or PTS2). To better understand the role of PEX5 in plant peroxisomal import, we characterized the Arabidopsis (Arabidopsis thaliana) pex5-10 mutant, which has a T-DNA insertion in exon 5 of the PEX5 gene. Sequencing results revealed that exon 5, along with the T-DNA, is removed in this mutant, resulting in a truncated pex5 protein. The pex5-10 mutant has germination defects and is completely dependent on exogenous Suc for early seedling establishment, based on poor utilization of seed-storage fatty acids. This …

A Comparison Of Cumulative-Germination Response Of Cheatgrass (Bromus Tectorum L.) And Five Perennial Bunchgrass Species To Simulated Field-Temperature Regimes, Stuart P. Hardegree, Corey A. Moffet, Bruce A. Roundy, Thomas A. Jones, Stephen J. Novak, Patrick E. Clark, Frederick B. Pierson, Gerald N. Flerchinger

Biology Faculty Publications and Presentations

Cheatgrass (Bromus tectorum L.) has come to dominate millions of hectares of rangeland in the Intermountain western United States. Previous studies have hypothesized that one mechanism conferring a competitive advantage to this species is the ability to germinate rapidly at low temperatures in the fall, winter and spring and, therefore, initiate growth and establishment more rapidly than more desirable perennial bunchgrass species. In this experiment, we developed thermal-germination-response models for multiple seedlots of cheatgrass and five perennial grass species. We conducted sensitivity analysis on potentialcumulative- germination response to a 38-y simulation of field-variable conditions of seedbed temperature and moisture. …

Description Of The Tadpole Of Proceratophrys Renalis (Miranda-Ribeiro, 1920) (Anura: Cycloramphidae), Filipe Augusto C. Do Nascimento, Barnagleison S. Lisboa, Gabriel O. Skuk, Rafael O. De Sá

Biology Faculty Publications

The tadpole of Proceratophrys renalis is described based on specimens from Maceió, State of Alagoas, northeastern Brazil. At stage 35 the body is slightly dorso-ventrally depressed, ovoid in lateral, dorsal, and ventral views. Oral disc is ventral with lateral emarginations, surrounded by a single row of marginal papillae with a large gap on the upper labium. Labial tooth row formula is 2(2)/3(1 ). The analysis of internal oral anatomy revealed two possible characters that readily distinguish P. renalis from P. boiei, supporting the recent resurrection of P. renal is. Comparisons with available descriptions of the larvae for other species in …

Cloning Of A Chlamydomonas Reinhardtii Marker Into A Rna Interference Construct To Test Whether The Photoreceptor Chlamyrhodopsin Is Involved In Circadian Clock Resetting, Shravya Reddy Maddi

Masters Theses & Specialist Projects

Chlamydomonas reinhardtii, a unicellular eukaryotic green alga, serves as a model organism to study the circadian clock in plants and animals. Rhodopsins are blue/green-light photoreceptors also found in C. reinhardtii. Chlamyrhodopsin (COP), the most abundant eyespot protein, was reported to have no role in the phototactic and photophobic responses in C. reinhardtii. Its function is yet unknown.
In the present study, we hypothesized that the function of COP is to mediate entrainment of the circadian clock by light. In order to test this hypothesis, a C. reinhardtii selection marker conferring resistance to the antibiotic paromomycin was cloned …

Labile Soil Carbon Inputs Mediate The Soil Microbial Community Composition And Plant Residue Decomposition Rates, Marie-Anne De Graaff, Aimee T. Classen, Hector F. Castro, Christopher W. Schadt

Biology Faculty Publications and Presentations

• Root carbon (C) inputs may regulate decomposition rates in soil, and in this study we ask: how do labile C inputs regulate decomposition of plant residues, and soil microbial communities?

• In a 14 d laboratory incubation, we added C compounds often found in root exudates in seven different concentrations (0, 0.7, 1.4, 3.6, 7.2, 14.4 and 21.7 mg C g soil) to soils amended with and without ¹³C-labeled plant residue. We measured CO₂ respiration and shifts in relative fungal and bacterial rRNA gene copy numbers using quantitative polymerase chain reaction (qPCR).

• Increased labile C input …

Hsp70 And Hsp40 Functionally Interact With Soluble Mutant Huntingtin Oligomers In A Classic Atp-Dependent Reaction Cycle, Gregor P. Lotz, Justin Legleiter, Rebecca Aron, Emily M. Sontag, Shao-Yi Huang, Cheping Ng, Charles G. Glabe, Leslie M. Thompson, Paul J. Muchowski

Biological Sciences Faculty Research and Publications

Inclusion bodies of aggregated mutant huntingtin (htt) fragments are a neuropathological hallmark of Huntington disease (HD). The molecular chaperones Hsp70 and Hsp40 colocalize to inclusion bodies and are neuroprotective in HD animal models. How these chaperones suppress mutant htt toxicity is unclear but might involve direct effects on mutant htt misfolding and aggregation. Using size exclusion chromatography and atomic force microscopy, we found that mutant htt fragments assemble into soluble oligomeric species with a broad size distribution, some of which reacted with the conformation-specific antibody A11. Hsp70 associated with A11-reactive oligomers in an Hsp40- and ATP-dependent manner and inhibited their …

Structure And Expression Of Two Nuclear Receptor Genes In Marsupials: Insights Into The Evolution Of The Antisense Overlap Between The Α-Thyroid Hormone Receptor And Rev-Erbα, Brandon C. Rindfleisch, M. Scott Brown, John L. Vandeberg, Stephen Munroe

Biological Sciences Faculty Research and Publications

Background: Alternative processing of α-thyroid hormone receptor (TRα, NR1A1) mRNAs gives rise to two functionally antagonistic nuclear receptors: TRα1, the α-type receptor, and TRα2, a non-hormone binding variant that is found only in mammals. TRα2 shares an unusual antisense coding overlap with mRNA for Rev-erbα (NR1D1), another nuclear receptor protein. In this study we examine the structure and expression of these genes in the gray short-tailed opossum, Monodelphis domestica, in comparison with that of eutherian mammals and three other marsupial species, Didelphis virginiana, Potorous tridactylus and Macropus eugenii, in order to understand the evolution and regulatory …