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Full-Text Articles in Life Sciences
Force-Velocity-Power And Force-Pca Relationships Of Human Soleus Fibers After 17 Days Of Bed Rest, Jeffrey J. Widrick, Kris M. Norenberg, C. A. Blaser, M. Karhanek, Jennifer J. Sherwood, Scott W. Trappe, Todd A. Trappe, David L. Costill, Robert H. Fitts
Force-Velocity-Power And Force-Pca Relationships Of Human Soleus Fibers After 17 Days Of Bed Rest, Jeffrey J. Widrick, Kris M. Norenberg, C. A. Blaser, M. Karhanek, Jennifer J. Sherwood, Scott W. Trappe, Todd A. Trappe, David L. Costill, Robert H. Fitts
Biological Sciences Faculty Research and Publications
Soleus muscle fibers from the rat display a reduction in peak power and Ca2+ sensitivity after hindlimb suspension. To examine human responses to non-weight bearing, we obtained soleus biopsies from eight adult men before and immediately after 17 days of bed rest (BR). Single chemically skinned fibers were mounted between a force transducer and a servo-controlled position motor and activated with maximal (isotonic properties) and/or submaximal (Ca2+ sensitivity) levels of free Ca2+. Gel electrophoresis indicated that all pre- and post-BR fibers expressed type I myosin heavy chain. Post-BR fibers obtained from one subject displayed increases in …
Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Biological Sciences: Faculty Publications
A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent …