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Full-Text Articles in Life Sciences

Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin Jan 2014

Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin

Turkish Journal of Biology

As a very invasive insect species, Lymantria dispar is adaptable and sensitive to a changing environment. In insects the neuroendocrine system first reacts to stress by production of prothoracicotropic neurohormones (PTTH) that control ecdysteroid synthesis (morphogenetic and stress hormones). In this article, we report changes in the L2' brain neurosecretory neurons that synthesize PTTH in L. dispar larvae after feeding on locust tree leaves (Robinia pseudoacacia), an unsuitable host plant. Groups of larvae (n = 20 per experimental group) were offered this in comparison with oak leaves (Quercus robur), a suitable control diet, for 3 days after molting into the …


The Effect Of Colcemid On The Heat Survival Of Mitotic V79 Chinese Hamster Cells, J. G. Szekely, G. P. Raaphorst, A. U. Lobreau, S. Delaney, E. I. Azzam Dec 1991

The Effect Of Colcemid On The Heat Survival Of Mitotic V79 Chinese Hamster Cells, J. G. Szekely, G. P. Raaphorst, A. U. Lobreau, S. Delaney, E. I. Azzam

Scanning Microscopy

V79 Chinese hamster cells were collected by colcemid addition to study the effect of heat on mitosis. When they were heated at 42°C and 45°C in the presence of 0.06 μg/mL colcemid, cell survival increased over the control samples, which were heated in ordinary medium. Scanning electron microscopy showed that cells heated to 45°C in the presence or absence of colcemid had fewer microvilli on the surface, but they did not have increased bleb formation. Transmission electron microscopy showed that the chromatin was diffuse in the heated cells and the kinetochores were indistinct. The mitochondria in the heated cells were …