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Full-Text Articles in Life Sciences
Optimizing Sperm Collection Procedures In Zebrafish, Madelyn B. Wasden, Rachel L. Roberts, April Delaurier
Optimizing Sperm Collection Procedures In Zebrafish, Madelyn B. Wasden, Rachel L. Roberts, April Delaurier
Journal of the South Carolina Academy of Science
Zebrafish are a highly-valued model organism used for developmental biology research. Zebrafish can be used for genetic manipulation and hence, many mutant and transgenic lines exist. It is impractical to maintain lines of adult zebrafish, due to resource constraints and the need to continuously produce new generations. Therefore, a practical way to preserve zebrafish lines is to freeze sperm and retrieve lines using in vitro fertilization of fresh eggs. Most existing in vitro protocols used by research labs have a wide variety of fertilization rates (ranging from 0% to >90%). Due to this variability, lines may be at risk of …
Ethical Concerns For Assisted Reproductive Technologies, Olga Najera
Ethical Concerns For Assisted Reproductive Technologies, Olga Najera
Dialogue & Nexus
The development of assisted reproductive technologies has provided new options for infertile couples in their pursuit of parenthood. As a result of the successful implementation of in vitro fertilization (IVF), gestational surrogacy is now an alternative. Other technology, such as genetic enhancement, could potentially become available for human beings soon; however, numerous ethical concerns have been raised by the fact that it requires germline engineering. The concerns brought about by these new reproductive technologies will be addressed in light of the teachings of the Roman Catholic Church and of the following ethical theories: Kantian, evolutionary, utilitarian, and virtue ethics.
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Turkish Journal of Biology
Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 …