Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
A Matter Of Regeneration And Repair: Caspases As The Key Molecules, Şehnaz Bolkent, Füsün Öztay, Selda Oktayoğlu, Serap Sancar Baş, Ayşe Karatuğ
A Matter Of Regeneration And Repair: Caspases As The Key Molecules, Şehnaz Bolkent, Füsün Öztay, Selda Oktayoğlu, Serap Sancar Baş, Ayşe Karatuğ
Turkish Journal of Biology
Researchers have been focused on understanding the pathogenesis of human diseases. They have been working to find the roles of caspases in the balance between apoptosis, autophagy, pyroptosis, and necroptosis, and also in the regeneration of damaged tissue. At this point, besides their death-inducing roles, new findings indicate the role of caspases in proliferation for maintaining the viability of cells in response to signals from apoptotic cells. Recently, determining cell fate has also been identified among other functions of caspases. Findings indicate that caspases direct cellular pathways to cell differentiation by suppressing stem cell self-renewal. The common opinion about the …
Human Embryonic Stem Cell N-Glycan Features Relevant To Pluripotency, Sabi̇re Karaçali
Human Embryonic Stem Cell N-Glycan Features Relevant To Pluripotency, Sabi̇re Karaçali
Turkish Journal of Biology
Human embryonic stem cells (hESCs) and reprogrammed human induced pluripotent stem cells (hiPSCs) proliferate indefinitely in the undifferentiated state (self-renewal) and differentiate into the three germ layers (and ultimately into all cell types) (pluripotency). Branching in the N-glycan core of hESCs/hiPSCs is limited. hESCs/hiPSCs have high mannose-type and biantennary complex-type core structures, indicating immature stages of N-glycoproteins. The two branches on the core consist of type 2 N-acetyllactosamine (LacNAc), completed mainly with α 2,6-linked sialic acid. Complex terminal fucosylation, particularly α 1,2-linked fucosylation, is another characteristic N-glycosylation feature of hESCs/hiPSCs. All of these structural features are probably associated with the …
Epigenetics: The Guardian Of Pluripotency And Differentiation, Marta Gladych, Aleksandra Nijak, Paula Lota, Urszula Oleksiewicz
Epigenetics: The Guardian Of Pluripotency And Differentiation, Marta Gladych, Aleksandra Nijak, Paula Lota, Urszula Oleksiewicz
Turkish Journal of Biology
Multicellular organisms comprise a variety of cell types that have the same genotype but distinct phenotypes. This diversity is possible because of the establishment of a specific epigenetic landscape that stabilizes gene expression profiles that are exclusive for a particular cell type. Accumulating data indicate that the maintenance, loss, and reacquisition of pluripotency are dynamically regulated by epigenetic alterations evoked by a subset of cellular factors. A better understanding of the epigenetic mechanisms involved in stem cell biology and differentiation will improve our ability to use these cells in the clinical context. Here we review current insights into the epigenetic …
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Turkish Journal of Biology
Current procedures used for skin injury reepithelization have yet to improve. At present, stem cell-based therapies provide great potential in repairing different damaged tissues due to the multipotent character of these cells. This study aimed to investigate the in vitro differentiation capacity of human adipose-derived stem cells (ASCs) into epidermal cells and to evaluate their interaction with HaCaT keratinocytes in coculture experimental models that mimicked the living environment of human skin. The mesenchymal phenotype of isolated stem cells was indicated by their spindle-shaped morphology and the expression of specific surface markers CD73, CD90, and CD105. After 21 days of cultivation …