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Full-Text Articles in Life Sciences
Evaluation Of Apoptotic Cell Death Mechanisms Induced By Hypericin-Mediated Photodynamic Therapy In Colon Cancer Cells, Aysun Kiliç Süloğlu, Eli̇f Karacaoğlu, Güldeni̇z Selmanoğlu, Hayri̇ye Akel, İbrahi̇m Çağatay Karaaslan
Evaluation Of Apoptotic Cell Death Mechanisms Induced By Hypericin-Mediated Photodynamic Therapy In Colon Cancer Cells, Aysun Kiliç Süloğlu, Eli̇f Karacaoğlu, Güldeni̇z Selmanoğlu, Hayri̇ye Akel, İbrahi̇m Çağatay Karaaslan
Turkish Journal of Biology
Hypericin (HYP)-mediated photodynamic therapy (PDT) is a new alternative treatment strategy for colon cancer inducing various cell-death pathways. Apoptotic cell death is the desired cellular fate in cancer cells. Therefore, we investigated the apoptotic pathways by determining apoptosis-related proteins (survivin, caspase-3, caspase-9, Bcl-2, and Bax) at the mRNA level using real-time polymerase chain reaction (qPCR), and the percentage of survivin was determined by survivin ELISA in HT-29 and Caco-2 colon cancer cell lines. The downregulation of survivin was significant 16 h after PDT for both cells, while caspase-3 upregulation was apparent 24 h after PDT. Caspase-9 and caspase-3 upregulations were …
Cytotoxicity And Antiviral Activity Of Palladium(Ii) And Platinum(Ii) Complexes With 2-(Diphenylphosphino)Benzaldehyde 1-Adamantoylhydrazone, Vera Simic, Stoimir Kolarevic, Ilija Brceski, Dejan Jeremic, Branka Vukovic-Gacic
Cytotoxicity And Antiviral Activity Of Palladium(Ii) And Platinum(Ii) Complexes With 2-(Diphenylphosphino)Benzaldehyde 1-Adamantoylhydrazone, Vera Simic, Stoimir Kolarevic, Ilija Brceski, Dejan Jeremic, Branka Vukovic-Gacic
Turkish Journal of Biology
Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect …