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Scanning Electron Microscopy Of The Celloidin-Embedded Inner Ear Sections, Kunihiro Mizuta, Tomoyuki Hoshino, Hirofumi Morita
Scanning Electron Microscopy Of The Celloidin-Embedded Inner Ear Sections, Kunihiro Mizuta, Tomoyuki Hoshino, Hirofumi Morita
Scanning Microscopy
The nerve fibers running inside the organ of Corti were studied in cats by scanning electron microscopy (SEM). The fixed temporal bones were decalcified and embedded in celloidin according to the conventional method. Thick serial sections (100-150 μm) were cut parallel to the basilar membrane. After removing the celloidin, the sections were freeze-dried in t-butyl alcohol. Though some outer hair cells had been deformed, the nerve fibers were well preserved for analysis.
Some new findings were observed in the present SEM study. The tunnel spiral bundle in a kitten took an atypical course on the floor of Corti's tunnel apart …