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Articles 1 - 30 of 91
Full-Text Articles in Life Sciences
Identification And Molecular Characterization Of A Receptor-Like Protein Kinase Gene From Corchorus Capsularis, Kh. Ambareen Reza, Sazia Sharmin, Mahdi Muhammad Moosa, Niaz Mahmood, Ajit Ghosh, Rifat Ara Begum, Haseena Khan
Identification And Molecular Characterization Of A Receptor-Like Protein Kinase Gene From Corchorus Capsularis, Kh. Ambareen Reza, Sazia Sharmin, Mahdi Muhammad Moosa, Niaz Mahmood, Ajit Ghosh, Rifat Ara Begum, Haseena Khan
Turkish Journal of Biology
A leucine-rich repeat-containing protein kinase (LRR-PK) gene has been identified in Corchorus capsularis. This gene, named CcLRR-RLK1, is 3032 bp long and encodes a protein composed of 958 amino acid residues. The deduced peptide contains 3 distinctive domains: a transmembrane domain, 5 leucine-rich repeat-containing motifs, and a serine/threonine protein kinase domain. Expression analysis indicated that the gene is reduced by several environmental stresses including high salt, fungus, and abscisic acid, suggesting its possible involvement in multiple stress response pathways.
A Holistic Approach For Selection Of Bacillus Spp. As A Bioremediator For Shrimp Postlarvae Culture, Thimmalapura Devaraja, Sanjoy Banerjee, Fatimah Yusoff, Mohamed Shariff, Helena Khatoon
A Holistic Approach For Selection Of Bacillus Spp. As A Bioremediator For Shrimp Postlarvae Culture, Thimmalapura Devaraja, Sanjoy Banerjee, Fatimah Yusoff, Mohamed Shariff, Helena Khatoon
Turkish Journal of Biology
Indigenous Bacillus pumilus, B. licheniformis, and B. subtilis were isolated from marine water and soil samples and investigated for potential bioremediation ability in Penaeus monodon culture. Bacillus spp. were selected based on their wide range of growth conditions, ease of mass culture, tolerance to total ammonia nitrogen (TAN), inhibition of pathogenic vibrios, nonpathogenicity, and ability to reduce TAN. Results showed that optimum growth of the selected Bacillus spp. occurred at 30 °C, pH 7.5, and 1.5% NaCl, and they secreted protease, amylase, and lipase. Vibrio spp. were also inhibited by 3 Bacillus spp. In addition, the selected Bacillus spp. had …
First Records And Microgeographical Variations Of Culturable Heterotrophic Bacteria In An Inner Sea (The Sea Of Marmara) Between The Mediterranean And The Black Sea, Turkey, Gülşen Altuğ, Mi̇ne Çardak, Peli̇n Sali̇ha Çi̇ftçi̇, Sevan Gürün
First Records And Microgeographical Variations Of Culturable Heterotrophic Bacteria In An Inner Sea (The Sea Of Marmara) Between The Mediterranean And The Black Sea, Turkey, Gülşen Altuğ, Mi̇ne Çardak, Peli̇n Sali̇ha Çi̇ftçi̇, Sevan Gürün
Turkish Journal of Biology
The microdiversity and composition of culturable heterotrophic aerobic bacteria were investigated in seawater samples taken from the Sea of Marmara (an important basin between the Mediterranean and the Black Sea) in different time periods throughout 2002-2010. The bacterial isolates were identified with the automated microidentification system VITEK 2 Compact 30 (bioMerieux, France). The compositions of identified bacteria according to their exposure to environmental factors in the areas from which they were isolated were compared. The primary hydrographic parameters (temperature, salinity, and dissolved oxygen) were recorded at the sampling station. The highest heterotrophic aerobic bacteria abundance was found in the coastal …
Bioaccumulation Of Tributyltin And Its Impact On Spermatogenesis In Mud Crab Scylla Serrata (Forskal), Revathi Peranandam, Iyapparaj Palanisamy, Munuswamy Natesan, Arockia Vasanthi Lourduraj, Krishnan Muthukalingan
Bioaccumulation Of Tributyltin And Its Impact On Spermatogenesis In Mud Crab Scylla Serrata (Forskal), Revathi Peranandam, Iyapparaj Palanisamy, Munuswamy Natesan, Arockia Vasanthi Lourduraj, Krishnan Muthukalingan
Turkish Journal of Biology
The effects of tributyltin (TBT) on the spermatogenesis of mud crab Scylla serrata were studied. TBT-exposed crabs exhibited a decrease in the gonadosomatic Index and the hepatosomatic Index compared to the crabs in the control group. Bioaccumulation of the TBT levels also varied in different tissues. The treated crabs showed variation in the weight of the testes with respect to TBT concentrations. Histological results clearly indicated that the control crabs showed spermatophores and granular substances were seen filling up the lumen of the seminiferous tubules. The testes of the experimental crabs illustrated that disruption in the seminiferous tubule membrane architecture, …
Effect Of Exogenously Applied Salicylic Acid On Cadmium Chloride-Induced Oxidative Stress And Nitrogen Metabolism In Tomato (Lycopersicon Esculentum L.), Esra Koç, Ayşen Sülün Üstün, Nuri̇ Çeli̇k
Effect Of Exogenously Applied Salicylic Acid On Cadmium Chloride-Induced Oxidative Stress And Nitrogen Metabolism In Tomato (Lycopersicon Esculentum L.), Esra Koç, Ayşen Sülün Üstün, Nuri̇ Çeli̇k
Turkish Journal of Biology
Salicylic acid (SA) has a key role in the formation of induced systemic resistance and hypersensitive cells, but it has also been indicated to be effective in the formation of strong responses against abiotic stresses such as drought, cold, heavy metal toxicity, and osmotic stress. This study has demonstrated a connection between the total soluble protein and the nitrate reductase (NR) activity. In 20 \muM CdCl_2, 40 \muM CdCl_2 + 0.5 mM SA, and 100 \muM CdCl_2 + 0.5 mM SA treatments, on the 1st day following the treatment an increase was detected in the NR activity when compared to …
Production Of Herbicide-Resistant Cowpea (Vigna Unguiculata L.) Transformed With The Bar Gene, Muhammad Aasim, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Production Of Herbicide-Resistant Cowpea (Vigna Unguiculata L.) Transformed With The Bar Gene, Muhammad Aasim, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Turkish Journal of Biology
Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement. The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz. The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25-0.75 mg L^{-1} 6-benzylaminopurine with or without 0.25 mg L^{-1} \alpha-naphthalene acetic acid. Shoot regeneration varied 44.4%-83.3% with 2.1-5.0 shoots per explant. Regenerated shoots were rooted in MS medium containing 0.50 mg L^{-1} indole-3-butyric acid and acclimatized in the greenhouse, where they flowered and set seeds. Immature cotyledons were transformed with …
Effect Of Genotypical Factors On The Effectiveness Of Anther Culture In Eggplant (Solanum Melongena L.), Sevi̇nç Başay, Şeküre Şebnem Elli̇altioğlu
Effect Of Genotypical Factors On The Effectiveness Of Anther Culture In Eggplant (Solanum Melongena L.), Sevi̇nç Başay, Şeküre Şebnem Elli̇altioğlu
Turkish Journal of Biology
The aim of this study was to determine the androgenic capacity of some eggplant (Solanum melongena L.) varieties and breeding lines, and to establish the effectiveness of androgenesis induction in the less responsive genotypes by crossing with more responsive genotypes that have a high ability for haploid embryo formation. Flower buds containing greenish-yellow anthers were collected when the microspores were at the late uninucleate stage, which is equivalent to a bud size where petals are not visible. The anthers were kept in the dark at 35 °C for 8 days, then incubated with a 12 h light period at 25 …
A Comparative Study Of Egf Effects On In Vitro Bovine Embryo Development In Monoculture And Sequential Media, Azadeh Zahmatkesh, Mahmood Amiri Roudbar, Morteza Daliri Joupari
A Comparative Study Of Egf Effects On In Vitro Bovine Embryo Development In Monoculture And Sequential Media, Azadeh Zahmatkesh, Mahmood Amiri Roudbar, Morteza Daliri Joupari
Turkish Journal of Biology
Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development. Oocytes were aspirated from 2- to 6-mm ovarian follicles and matured in maturation media. After in vitro fertilization of matured oocytes, presumptive zygotes were cultured in test media with or without additional exogenous EGF. Results indicated that EGF significantly increased blastocyst formation but not cleavage rate in both media. There was …
In Vitro Mutagenesis Of Etlingera Elatior (Jack) And Early Detection Of Mutation Using Rapd Markers, Muhamad Fahmi Yunus, Maheran Abd Aziz, Mihdzar Abdul Kadir, Siti Khalijah Daud, Azmi Abdul Rashid
In Vitro Mutagenesis Of Etlingera Elatior (Jack) And Early Detection Of Mutation Using Rapd Markers, Muhamad Fahmi Yunus, Maheran Abd Aziz, Mihdzar Abdul Kadir, Siti Khalijah Daud, Azmi Abdul Rashid
Turkish Journal of Biology
Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD_{50} to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found …
The Effect Of Hydrogen Peroxide/Colloidal Silver On Reducing The Colonization And Growth Of Heterotrophic Bacteria In Dental Unit Waterlines, Meral Özalp, Ömer Engi̇n Bulut, Ati̇lla Stephan Ataç, Meli̇ke Eki̇zoğlu, Di̇dem Kart, Hakan Hamdi̇ Çeli̇k, İlkan Tatar
The Effect Of Hydrogen Peroxide/Colloidal Silver On Reducing The Colonization And Growth Of Heterotrophic Bacteria In Dental Unit Waterlines, Meral Özalp, Ömer Engi̇n Bulut, Ati̇lla Stephan Ataç, Meli̇ke Eki̇zoğlu, Di̇dem Kart, Hakan Hamdi̇ Çeli̇k, İlkan Tatar
Turkish Journal of Biology
The objective of the present study was to investigate reduction in the colonization and growth of heterotrophic bacteria in dental unit waterlines by using hydrogen peroxide/colloidal silver as disinfectant. Twenty-seven dental units were included; 6 units that were more than 20 years old and 7 units that had been in use for 2 years comprised the old and new treatment groups, respectively. Fourteen units served as controls. The treatment groups were disinfected continuously and every 4 weeks shock doses were applied over a 20-week period. Water samples were taken before treatment, 1 and 2 weeks after treatment, and thereafter every …
A Cellulase-Free, Thermoactive, And Alkali Xylanase Production By Terrestrial Streptomyces Sp. Ca24, İlknur Porsuk, Süleyman Özakin, Bülent Bali̇, Ebru İnce Yilmaz
A Cellulase-Free, Thermoactive, And Alkali Xylanase Production By Terrestrial Streptomyces Sp. Ca24, İlknur Porsuk, Süleyman Özakin, Bülent Bali̇, Ebru İnce Yilmaz
Turkish Journal of Biology
Microbial xylanases have attracted much attention because of their applications in industrial processes during the past few decades. Sixty-five terrestrial actinomycetes previously isolated from rhizospheric soils of endemic plants were tested for their ability in production of xylanase. The results revealed that 33 isolates could degrade xylan on both solid and liquid media. Extracellular xylanase production by these isolates was characterized during their growth in liquid medium. Streptomyces sp. CA24, one of the most active strains, was selected for optimization of xylanase production. The enzyme secretion was enhanced when the medium was supplemented with oat spelt xylan (0.5%) and yeast …
Purification And Biochemical Characterization Of An Extracellular Lipase From Psychrotolerant Pseudomonas Fluorescens Ke38, Aysun Adan Gökbulut, Alper Arslanoğlu
Purification And Biochemical Characterization Of An Extracellular Lipase From Psychrotolerant Pseudomonas Fluorescens Ke38, Aysun Adan Gökbulut, Alper Arslanoğlu
Turkish Journal of Biology
An extracellular lipase producing bacterium was isolated from a soil sample, and identified as a strain of Pseudomonas fluorescens by 16S rRNA gene sequencing. It was named Pseudomonas fluorescens KE38. KE38 showed psychrotolerant properties with an optimum growth temperature of 25 °C. The lipase enzyme secreted by KE38 was purified 41.13-fold with an overall yield of 54.99%, and a specific activity of 337.3 U/mg. The molecular mass of purified lipase was estimated to be approximately 43 kDa by SDS-PAGE. Although the lipase was active at a temperature range of 1565 °C, it exhibited maximum activity at 45 °C, at pH …
Characterization, Antifungal Activity, And Cell Immobilization Of A Chitinase From Serratia Marcescens Mo-1, Sezer Okay, Murat Özdal, Esabi̇ Başaran Kurbanoğlu
Characterization, Antifungal Activity, And Cell Immobilization Of A Chitinase From Serratia Marcescens Mo-1, Sezer Okay, Murat Özdal, Esabi̇ Başaran Kurbanoğlu
Turkish Journal of Biology
Chitinases have the potential to control many pathogen species, such as fungi containing chitin on their cell walls. In the present study, chitinase from novel isolate Serratia marcescens MO-1, isolated from Poecilimon tauricola (Orthoptera: Tettigoniidae) in Turkey, was investigated. The optimum pH and temperature for the chitinase activity were found to be pH 7.0 (36.6 U/mL) and 50 °C (37.1 U/mL), respectively. Moreover, the activity was still high in acidic (23.0 U/mL at pH 3.0) and basic (17.3 U/mL at pH 11.0) conditions as well as at lower (29.5 U/mL at 20 °C) and higher (28.7 U/mL at 80 °C) …
Optimization Of Immobilization Conditions By Conventional And Statistical Strategies For Alkaline Lipase Production By Pseudomonas Aeruginosa Mutant Cells: Scale-Up At Bench-Scale Bioreactor Level, Deepali Bisht, Santosh Kumar Yadav, Nandan Singh Darmwal
Optimization Of Immobilization Conditions By Conventional And Statistical Strategies For Alkaline Lipase Production By Pseudomonas Aeruginosa Mutant Cells: Scale-Up At Bench-Scale Bioreactor Level, Deepali Bisht, Santosh Kumar Yadav, Nandan Singh Darmwal
Turkish Journal of Biology
Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, …
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Turkish Journal of Biology
The cytotoxicity of 6 highly cytotoxic Pseudomonas aeruginosa isolates was tested against Vero cells. Cytotoxicity was found to be cell-associated, high cytotoxicity was observed at a multiplicity of infection of 200:1, and this activity gradually decreased as the bacterial count decreased. This cytotoxicity caused rounding and detachment of the Vero monolayer as early as 1 h after infection. Moreover, viable metabolically active bacteria were necessary for Vero cell killing. These results imply that one or more of the type III secretion system effector proteins (ExoU, ExoS, ExoT, or ExoY) were responsible for the observed cytotoxic effects. This cytotoxicity was affected …
Toxicities Of Diuron And Irgarol On The Hatchability And Early Stage Development Of Artemia Salina, Hakan Alyürük, Levent Çavaş
Toxicities Of Diuron And Irgarol On The Hatchability And Early Stage Development Of Artemia Salina, Hakan Alyürük, Levent Çavaş
Turkish Journal of Biology
Booster biocides are widely used in antifouling paints as bioactive agents against fouling organisms. In previously published reports, acute toxicity tests on Artemia salina (Linnaeus, 1758) were only focused on a part of the life cycle of the organism. The aim of this study was to investigate the toxicities of diuron and irgarol on the hatching stage of A. salina. According to the results, diuron significantly decreased the hatching percentage of A. salina cysts and prevented the hatching of larvae. The EC_{50} value for diuron was found as 12.01 mg/L. On the other hand, irgarol had no effect on the …
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Turkish Journal of Biology
This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheeps-blood agar plates. The differences in antigrowth …
Genotoxic And Safety Assessment Of 2 Parabens In Somatic Cells Of In Vivo Drosophila Melanogaster, Ari̇f Ayar, Handan Uysal
Genotoxic And Safety Assessment Of 2 Parabens In Somatic Cells Of In Vivo Drosophila Melanogaster, Ari̇f Ayar, Handan Uysal
Turkish Journal of Biology
The objective of this study was to determine the possible genotoxic effects of the para-hydroxybenzoic acid esters (parabens) of methylparaben, propylparaben, and the mixed paraben groups formed by a combination of these, which are used as preservative substances in the food, cosmetic, and drug industries, on Drosophila melanogaster using the wing somatic mutation and recombination test (SMART). According to the data obtained from microscopic analysis, no increase was determined in the single type clone number at various concentrations (100, 150, 200, and 250 mM) of paraben groups in comparison with the control group. In addition, increases corresponding to the concentration …
Stimulation Of Dendritic Cells With Vaccine And Vaccine-Antibody Complex And Effect On Immune Response, İbrahi̇m Hati̇poğlu, İbrahi̇m Söğüt, Duygu Ercan, Soner Aksu, Hülya Si̇vas, Aynur Başalp
Stimulation Of Dendritic Cells With Vaccine And Vaccine-Antibody Complex And Effect On Immune Response, İbrahi̇m Hati̇poğlu, İbrahi̇m Söğüt, Duygu Ercan, Soner Aksu, Hülya Si̇vas, Aynur Başalp
Turkish Journal of Biology
Dendritic cell (DC) vaccines are a promising and potent therapeutic tool for chronic diseases, autoimmune diseases, and cancer because of the unique ability of DCs to stimulate T cells. The challenge of DC vaccines is to find an effective form for antigen presentation. Although pure antigens, antigen complexes, plasmids, and mRNA have been used in different studies, no proper application to overcome this problem has been found yet. In this study, we investigated the eligibility of a commercial hepatitis B virus (HBV) vaccine or a vaccine-monoclonal antibody complex for antigen loading of DCs for a therapeutic purpose. DCs were derived …
The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada
The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada
Turkish Journal of Biology
The aim of this study was to produce laccase enzymes by using various lignocellulosic wastes (LCWs) under semisolid-state (SsF) and submerged fermentation (SF) conditions. White rot fungi are the best laccase-producing organisms and they can be easily grown on LCW. Thus, in this work, Trametes trogii (Berk.) ATCC 200800 and Trametes versicolor (L.) ATCC 200801, well-known laccase-producing white rot fungi, were used as laccase-producing organisms. According to the literature, some of the LCWs such as sunflower receptacle, apricot seed shell, and bulrush were tested first as substrates for laccase production by these fungi. In SsF, the maximum laccase activities were …
Occurrence Of Enterocin Genes, Virulence Factors, And Antibiotic Resistance In 3 Bacteriocin-Producer Enterococcus Faecium Strains Isolated From Turkish Tulum Cheese, Banu Özden Tuncer, Zeli̇ha Ay, Yasi̇n Tuncer
Occurrence Of Enterocin Genes, Virulence Factors, And Antibiotic Resistance In 3 Bacteriocin-Producer Enterococcus Faecium Strains Isolated From Turkish Tulum Cheese, Banu Özden Tuncer, Zeli̇ha Ay, Yasi̇n Tuncer
Turkish Journal of Biology
In this study, the occurrence of enterocin genes, virulence factors, and antibiotic resistance in the bacteriocin-producer Enterococcus faecium EYT17, EYT31, and EYT39 strains was investigated. Polymerase chain reaction (PCR) studies showed that all of the E. faecium strains carried the enterocin A and B structural genes (entA and entB), while E. faecium EYT17 and EYT31 strains also carried the enterocin P structural gene (entP). None of the strains exhibited \beta-haemolysis or gelatinase activity. PCR analysis revealed that EYT17, EYT31, and EYT39 strains were clear of potential virulence determinants, except for ccf and efaAfm. The ccf gene was found in all …
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Turkish Journal of Biology
Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccards coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR …
Effect Of Drought Stress On Oxidative Damage And Antioxidant Enzyme Activity In Melon Seedlings, Musa Kavas, Mehmet Cengi̇z Baloğlu, Oya Akça, Fatma Seli̇n Köse, Derya Gökçay
Effect Of Drought Stress On Oxidative Damage And Antioxidant Enzyme Activity In Melon Seedlings, Musa Kavas, Mehmet Cengi̇z Baloğlu, Oya Akça, Fatma Seli̇n Köse, Derya Gökçay
Turkish Journal of Biology
Drought stress is one of the most serious environmental limitations affecting the growth and productivity of plants. In the current study, oxidative damage and antioxidant responses under polyethylene glycol (PEG)-induced osmotic stress were compared in 2 melon cultivars, Kırkağaç and Galia. Melon seedlings were subjected to PEG-6000 solutions of 2 different osmotic potentials, -0.2 MPa and -0.4 MPa. Various physiological parameters, malondialdehyde (MDA), proline content, and antioxidant enzymes including catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were measured. A reduction in fresh and dry weights of shoot and root tissues was observed. Significant proline accumulation was detected with …
Probing The Architecture Of Testis And Morphology Of Male Germinal Cells In The Mud Crab With The Atomic Force Microscopy, Mahalingam Anbarasu, Ramangalingam Kirubagaran, Gopal Dharani, Thanumalaya Subramoniam
Probing The Architecture Of Testis And Morphology Of Male Germinal Cells In The Mud Crab With The Atomic Force Microscopy, Mahalingam Anbarasu, Ramangalingam Kirubagaran, Gopal Dharani, Thanumalaya Subramoniam
Turkish Journal of Biology
The architecture of testicular lobules, morphology of spermatozoa, and spermatophore in the mud crab, Scylla serrata, are described for the first time employing atomic force microscopy (AFM). A fast and simple squash preparation protocol was followed using crustacean physiological saline without distorting the natural structure. Observations provided new insight into the arrangement of lobules and morphology of testicular assembly. It consists of a close network of lobules of different sizes in a radial manner on radially branching basal tubules. Budding of new lobules is another distinguishable feature noted in the network of testicular organization. A thin membranous envelope of the …
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Turkish Journal of Biology
The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined …
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Turkish Journal of Biology
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological …
Global Assessment Of Network Inference Algorithms Based On Available Literature Of Gene/Protein Interactions, Gökmen Altay, Nejla Altay, David Neal
Global Assessment Of Network Inference Algorithms Based On Available Literature Of Gene/Protein Interactions, Gökmen Altay, Nejla Altay, David Neal
Turkish Journal of Biology
We propose a framework that uses the available gene/protein interaction databases of the literature as a universal benchmark in order to globally assess the inference performances of gene network inference algorithms. We also developed an R software package for convenient use of the framework, which can also be used in general as a quick tool to search in the literature for available validations of interactions. We applied the proposed approach to 2 publicly available prostate cancer gene expression datasets and a large breast cancer gene expression dataset. The results revealed different aspects and superiority of algorithms that had not been …
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Turkish Journal of Biology
The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. …
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Turkish Journal of Biology
Iron is an essential nutrient for almost all organisms, but it is also a potent toxin because it can catalyze oxidative reactions that are destructive to cells. Transferrin is an essential protein involved in iron metabolism, immunity, and vitellogenesis in insects. We have characterized a cDNA encoding a putative iron-binding transferrin (SpliTrf) in Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae), an important pest that causes extensive losses in many vegetable, fodder, and fiber crops. The structure of the putative SpliTrf showed significant homology to other insect transferrins. Gene expression of SpliTrf was examined from different adult tissues including the …
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Turkish Journal of Biology
Yeast transformations with Brassica napus and Perilla frutescens \omega-3 desaturases were carried out. Feeding experiments on exogenous 18:2 at 10 mg/L and 100 mg/L for both genes under this investigation showed the same activity levels over the 3 days of the incubation, producing almost the same amount of 18:3. To further investigate the characteristics of B. napus and P. frutescens \omega -3 desaturases, a time-course in vivo activity study was conducted. Both enzymes showed the same behavior over the time course, desaturating 18:2 into 18:3. Moreover, the use of 2 different temperatures for incubation (30 °C and 20 °C) did …