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Articles 1 - 30 of 48
Full-Text Articles in Life Sciences
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Purification Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Meti̇n Yildirim
Turkish Journal of Biology
Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
Optimization Of Pcr Amplification Of Wheat Simple Sequence Repeat Dna Markers, Nurdan Doğrar, Mahi̇nur S. Akkaya
Optimization Of Pcr Amplification Of Wheat Simple Sequence Repeat Dna Markers, Nurdan Doğrar, Mahi̇nur S. Akkaya
Turkish Journal of Biology
Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleotide tandem repeats containing loci of eukaryotic genomes. It is demonstrated that these loci are very polymorphic due to the change in the number of repeating units among the individuals of populations. Each SSR locus can easily be amplified by using a polymerase chain reaction (PCR) knowing the DNA sequence flanking the repeat region specifically. The only limiting feature of the application of these markers is the need for prior sequence information for developing primers in locus-specific PCR amplification. This limitation is alleviated for the economically important species and …
Molecular Characterization Of The Gene Encoding For The Salivaricin B Activity And Its Flanking Sequences, Osman Çataloluk
Molecular Characterization Of The Gene Encoding For The Salivaricin B Activity And Its Flanking Sequences, Osman Çataloluk
Turkish Journal of Biology
Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and …
Isolation And Characterization Of Pediocin Producing Pediococcus Pentosaceus Pep1 From Vacuum-Packed Sausages, Özlem Osmanağaoğlu, Yavuz Beyatli, Ufuk Gündüz
Isolation And Characterization Of Pediocin Producing Pediococcus Pentosaceus Pep1 From Vacuum-Packed Sausages, Özlem Osmanağaoğlu, Yavuz Beyatli, Ufuk Gündüz
Turkish Journal of Biology
Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages, and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. Gram-negative organisms such as Yersinia enterocolitica and Escherichia coli were not inhibited by this antimicrobial agent. Since the antimicrobial agent was found to be sensitive to proteolytic enzymes, resistant to heat and organic solvents and active over a wide range of pH values, between 3 and 8, apparently most stable in the lower …
Nucleotypic Effects In Different Genotypes Of Vicia Sativa L., Serap Çeli̇kler, Rahmi̇ Bi̇laloğlu
Nucleotypic Effects In Different Genotypes Of Vicia Sativa L., Serap Çeli̇kler, Rahmi̇ Bi̇laloğlu
Turkish Journal of Biology
Seven cultivar varieties of Vicia sativa L. were used. Nucleotypic effects of telophase (2C) nuclear DNA amounts on seed weight, percent mitotic index, stomatal frequency/mm^2, chlorophyll a and b content, root growth and shoot growth were determined. No correlation was observed between the DNA contents and the percent mitotic index, chlorophyll a and b content, or root growth of the cultivar varieties. However a significant correlation was observed between DNA content and the seed weight, stomatal frequency and shoot growth of the cultivar varieties.
Determination Of The Dna And Amino Acid Sequences Of The Lactate Dehydrogenase Gene From Plasmodium Falciparum Strains K1 And Pf Fcbr: A Route To The Design Of New Antimalarials, Di̇lek Turgut Balik, J. John Holbrook
Determination Of The Dna And Amino Acid Sequences Of The Lactate Dehydrogenase Gene From Plasmodium Falciparum Strains K1 And Pf Fcbr: A Route To The Design Of New Antimalarials, Di̇lek Turgut Balik, J. John Holbrook
Turkish Journal of Biology
This paper describes cloning of the gene coding for the lactate dehydrogenase (LDH) gene from the two different strains of Plasmodium falciparum, K1 and PF FCBR. The DNA sequences of LDH genes of these two strains were found to be identical. Amino acid sequence alignment of LDH from P. falciparum strains K1 and PF FCBR (PfLDH) to some other known LDH sequences showed that PfLDH has 29% residue identities with Bacillus stearothermophilus (BsLDH) and 29%, 31%, 33% with dogfish, man-M_4, and pig-M_4 LDHs, respectively. It was also shown that PfLDH has insertions and single amino acid deletions. Two deletions are …
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Turkish Journal of Biology
Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci …
Callus Induction And Plant Regeneration From Mature Embryos Of Oat (Avena Sativa L.), Melahat A.Bi̇rsi̇n, Sertaç Önde, Murat Özgen
Callus Induction And Plant Regeneration From Mature Embryos Of Oat (Avena Sativa L.), Melahat A.Bi̇rsi̇n, Sertaç Önde, Murat Özgen
Turkish Journal of Biology
The aim of this study, which was conducted in Ankara University, Faculty of Agriculture, Department of Field Crops, biotechnology laboratory in 1998-1999, was to determine the callus induction and plant regeneration capacity of oat (Avena sativa L.). The mature embryos of 10 oat genotypes were utilised for callus induction. The statistical analysis of the results revealed that callus induction and plant regeneration capacity were dependent on the oat genotype. However, a significant relationship was not established between callus induction and regeneration capacity and, furthermore, the number of regenerated plants was determined to be directly related to the callus production capacity.
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Turkish Journal of Biology
In recent years, cell adhesion and cell adhesion molecules have been shown to be important for many normal biological processes, including embryonic cell migration, immune system functions and wound healing. It has also been shown that they contribute to the pathogenesis of a large number of common human disorders, such as rheumatoid arthritis and tumor cell metastasis in cancer. In this review, the basic mechanisms of cellular adhesion and the structural and functional features of adhesion molecules are summarized.
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
Turkish Journal of Biology
A quantitative test to study Listeria and Salmonella adherence to epithelial cells was developed. The quantitative test is rapid and allows the simultaneous testing of many variables such as the adherence ability of different bacteria to their target cells as well as the capability of various molecules to inhibit bacterial adherence. By using a strain-specific standard curve in each test, the test for the quantification of adherent bacteria became specifically sensitive. Non-viable biotinylated bacteria and immobilized cells or their extract were found relevant for the study of bacteria-epithelial cell interactions. Adherence ability of Listeria strains was found to be 10-fold …
Effects Of Isochronal Exogenously Applied Synthetic-Ga_3, Fungal-Gibberellic Acid And Red Light On The Sheath Length Of The Second Leaf Of Oryza Sativa L.Cv. Tan-Ginbozu, Ş. Fati̇h Topçuoğlu, Serpi̇l Ünyayar
Effects Of Isochronal Exogenously Applied Synthetic-Ga_3, Fungal-Gibberellic Acid And Red Light On The Sheath Length Of The Second Leaf Of Oryza Sativa L.Cv. Tan-Ginbozu, Ş. Fati̇h Topçuoğlu, Serpi̇l Ünyayar
Turkish Journal of Biology
The effects of isochronal exogenously applied synthetic-GA_3, fungal-gibberellic acid and different light on the sheath length of the second leaf of Oryza sativa L.cv.Tan-ginbozu were determined under controlled environmental conditions. Our results showed that the effect of fungal-gibberellic acid on the sheath length of the second leaf is higher than that on the control groups (group of 1/2N Hoagland culture solution and group of 1/2N Hoagland culture solution+methanol); however, synthetic-GA_3 was more effective on the length of the second leaf than fungal gibberellic acid. Moreover, it was identified that the effect of red light is higher than that of fluorescent …
Characterization Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Zeli̇ha Yildirim, Meti̇n Yildirim
Characterization Of Buchnericin Lb Produced By Lactobacillus Buchneri Lb, Zeli̇ha Yildirim, Meti̇n Yildirim
Turkish Journal of Biology
Lactobacillus buchneri LB isolated from a commercially fermented vegetable product produced a bacteriocin-designated buchnericin LB. It was found that buchnericin LB was heat-stable (90-121^oC for 15 min) and hydrolyzed by proteases, \alpha-chymotrypsin, trypsin, papain, proteinase K and pepsin. However, it was observed that it was resistant to catalase, peroxidase, lipase, amylases and organic solvents. Also, it was found that it retained its biological activity after exposure to pH 2.0-9.0 and after storage at -20^o and -70^oC. It was determined that buchnericin LB had a wide inhibitory spectrum, being inhibitory to the Listeria, Bacillus, Enterococcus, Micrococcus, Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus …
Effects Of Sewage Pollution The Structure Of The Community Of Ulva Lactuca, Enteremorpha Linza And Rocky Macrofauna In Dışliman Of Sinop, Levent Bat, Mehmet Akbulut, Murat Sezgi̇n, Mehmet Çulha
Effects Of Sewage Pollution The Structure Of The Community Of Ulva Lactuca, Enteremorpha Linza And Rocky Macrofauna In Dışliman Of Sinop, Levent Bat, Mehmet Akbulut, Murat Sezgi̇n, Mehmet Çulha
Turkish Journal of Biology
Untreated sewage and domestic wastes from the Sinop outfall enter the Dışliman region. The effect of this on the distribution of Ulva lactuca, Enteromorpha linza and benthic macrofauna and on enviromental parameters was determined. Increases in the growth of opportunistic green algae such as Ulva lactuca and Enteromorpha linzain response to increased nutrient supply in the Dışliman coastal ecosystems in Sinop are reported in the present study. The results of this study show that organic enrichment also increases, with Mytilus galloprovincialis (Mollusca), Erichthonius brasiliensis, Echinogammarus olivii and Amphitoes ramondi, Idotea baltica and Sphaeroma serratum(Crustacea) being the most abundant species.
Effects Of High Dietary Protein And High Energy Density On Gastric Evacuation And Return Of Appetite In The Rainbow Trout, Oncorhynchus Mykiss, Ahmet Adem Teki̇nay, Yusuf Güner
Effects Of High Dietary Protein And High Energy Density On Gastric Evacuation And Return Of Appetite In The Rainbow Trout, Oncorhynchus Mykiss, Ahmet Adem Teki̇nay, Yusuf Güner
Turkish Journal of Biology
Rainbow trout (Oncorhynchus mykiss) were fed diets containing three different concentrations of crude protein (56.3%, 51.7% and 45.8%) and digestible energy (21.3, 20.3 and 18.8 MJ/kg), and the effects of dietary protein and energy level on gastric evacuation and return of appetite times were investigated. Gastric evacuation determinations were achieved by slaughtering 8 fish every 6 hours until no residue was found in the cardiac stomach of fish. Return of appetite experiments were conducted by re-feeding groups of trout every 4 or 6 hours following the first feeding. The data obtained from each process was modelled by regression analysis and …
Antiviral And Cytotoxic Activities Of Extracts From The Cell Cultures And Respective Parts Of Some Turkish Medicinal Plants, Atalay Sökmen
Antiviral And Cytotoxic Activities Of Extracts From The Cell Cultures And Respective Parts Of Some Turkish Medicinal Plants, Atalay Sökmen
Turkish Journal of Biology
Extracts from respective parts and cell cultures of some Turkish medicinal plants have been assessed for their antiviral and cytotoxic properties. None of the extracts tested showed notable activity against herpes simplex viruses (HSV-I and II), but a slight antiretroviral activity against HIV-I was determined in an extract from Hypericum capitatum cell cultures. On the other hand, according to cytotoxic activity test results against brine shrimp (Artemia salina), an activity level of LD_{50} at 34.9 µg/ml was found on the extracts of Ecbalium elaterium cell cultures.
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), is a serious pest of pine plantations and ornamentals. Chemical pesticides used to control this pest have a detrimental effect on the environment. The Neodiprion sertifer nuclear polyhedrosis virus (NsNPV) frequently causes considerable decreases in the populations but doses not harm other animals or plants. In this study, we tested five different concentrations of NsNPV against the larvae of N. sertifer and showed viral infection using Giemsa staining. When a viral concentration of 5x10^9 PIB/ml (Polyhedral Inclusion Body) was utilized, larval mortality started on the fourth day and reached 90.4% on …
In Vitro Inhibition Of The Mycelial Growth Of Some Root Rot Fungi By Rhizobium Leguminosarum Biovar Phaseoli Isolates, İbrahi̇m Özkoç, Muhammet Hani̇fi̇ Deli̇veli̇
In Vitro Inhibition Of The Mycelial Growth Of Some Root Rot Fungi By Rhizobium Leguminosarum Biovar Phaseoli Isolates, İbrahi̇m Özkoç, Muhammet Hani̇fi̇ Deli̇veli̇
Turkish Journal of Biology
In solid medium, the effects of twenty-three Rhizobium leguminosarum biovar phaseoli isolates on the mycelium development of the phytopathogenic fungi of three different genera (Fusarium oxysporum, Pythium ultimum and Rhizoctonia solani) were tested in vitro. The experiments were achieved by applying the superimpose dual culture technique. Most of the rhizobia tested inhibited the fungal development. The inhibition rates displayed differences in accordance with the bacteria and fungi isolates utilized. However, the inhibitory effect of the rhizobia occurred most on the isolates of Fusarium and Pythium species (up to 30.35% and 29.75% respectively) and least on those of Rhizoctonia species (16.03%). …
Effect Of Some Organophosphorus Insecticides On Soil Microorganisms, Meti̇n Diğrak, Ferdağ Kazanici
Effect Of Some Organophosphorus Insecticides On Soil Microorganisms, Meti̇n Diğrak, Ferdağ Kazanici
Turkish Journal of Biology
The effect of some organophosphorus insecticides [Isofenphos (=Oftanol DS 40), Phorate (=Thimet 10G and Foratox 10-G) and Fonofos] were investigated on soil microbiota (total viable bacteria, actinomycetes, anaerobic bacteria, aerobic endospores, proteolytic bacteria, cellülolytic microorganisms and yeast-mold). In the isofenphos-treated soil sample, the total viable bacteria number was found to be higher than that of the control groups during incubation. Moreover, it was observed that this treatment had no inhibitory effect on the development of the other microorganism groups. It was determined that the fonofos and phorate pesticides were had no inhibitory effect on the devel- opment of the soil …
Blood Serum Proteins Of Apodemus Flavicollis And Apodemus Hermonensis (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Ercüment Çolak, Nuri̇ Yi̇ği̇t, Mustafa Sözen, Şaki̇r Özkurt
Blood Serum Proteins Of Apodemus Flavicollis And Apodemus Hermonensis (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Ercüment Çolak, Nuri̇ Yi̇ği̇t, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
The globulin, albumin, postalbumin, and prealbumin proteins of Apodemus flavicollis and Apodemus hermonensiswere examined by SDS-PAGE. In A. flavicollis and A. hermonensis respectively, 6-9 and 7-9 electrophoretic bands were determined in the globulin region, respectively. Both species have one band in the postalbumin zone, and two bands in the prealbumin zone. A. flavicollis differs from A. hermonensisin terms of postalbumin.
Transient Eexpression Of B-Glucuronidase Reporter Gene In Sainfoin (Onobrychis Viciifolia Scop.) Cotyledons Via Microprojectile Bombardmen, Sertaç Önde, Cengi̇z Sancak, Suzan Altinok, Melahat Bi̇rsi̇n, Murat Özgen
Transient Eexpression Of B-Glucuronidase Reporter Gene In Sainfoin (Onobrychis Viciifolia Scop.) Cotyledons Via Microprojectile Bombardmen, Sertaç Önde, Cengi̇z Sancak, Suzan Altinok, Melahat Bi̇rsi̇n, Murat Özgen
Turkish Journal of Biology
Transient \beta-glucuronidase reporter gene (GUS) expression was visualised in whole cotyledons of sainfoin (Onobrychis viciifolia Scop.) after microprojectile bombardment with pBI221.23 DNA. Optimum rupture disk pressure and sample plate distance were determined to be 1350 psi and 6 cm, respectively. Mean gold particle diameter of 1.0 µm and 48 h post-bombardment histochemical GUS assay was found to be superior to 1.6 µm gold particles and 120 h post-bombardment GUS assay. Microscopic analysis revealed the localisation of the gold particles predominantly in the epidermal cell layer, whereas less than 1% of the particles were localised in sub-epidermal cells, as deep as …
The Effect Of Paraquat On The Activity Of Some Enzymes In Different Tissues Of Mice (Mus Musculus - Swiss Albino), Egemen Dere, Fi̇kri̇ye Polat
The Effect Of Paraquat On The Activity Of Some Enzymes In Different Tissues Of Mice (Mus Musculus - Swiss Albino), Egemen Dere, Fi̇kri̇ye Polat
Turkish Journal of Biology
The effects of the LD_{30} (20 mg.kg^{-1}) dose of paraquat, a herbicide, were investigated on creatine kinase (CK), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) activities in the liver, kidney and lung of mice after 2, 4, 8, 16, 32, 64 and 72 hours. After paraquat was given intraperitoneally to Mus musculus Swiss albino mice, the control groups were injected with physiological saline. The results were evaluated using the autoanalyzer as U/L. Even though significant increases in the liver tissue LDH and GGT, kidney tissue CK and lung tissue CK and …
Pulsed-Field Gel Electrophoresis (Pfge) Technique And Its Use In Molecular Biology, Esi̇n Basim, Hüseyi̇n Basim
Pulsed-Field Gel Electrophoresis (Pfge) Technique And Its Use In Molecular Biology, Esi̇n Basim, Hüseyi̇n Basim
Turkish Journal of Biology
In recent years, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology area has been subject to much research. PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria that are poorly understood at the genetic level as well as in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE also has advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths. In this …
Blood-Serum Proteins Of Rattus Rattus And Rattus Norvegicus (Mammalia: Rodentia) In Turkey, Nuri̇ Yi̇ği̇t, Reyhan Çolak, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Blood-Serum Proteins Of Rattus Rattus And Rattus Norvegicus (Mammalia: Rodentia) In Turkey, Nuri̇ Yi̇ği̇t, Reyhan Çolak, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
The blood serum proteins of 35 live specimens of Rattus rattus and Rattus norvegicus collected from ten localities in Turkey were examined by SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis). Globulin, albumin, postalbumin, and prealbumin proteins in blood serum showed different electrophoretic patterns in populations of R. rattusand R. norvegicus.Because of the variations in electrophoretic patterns of both species, these patterns do not seem to be diagnostic characters in distinguishing R. rattus from R. norvegicus.
Effects Of Cd^{2+}, Zn^{2+}, Li^+ And Al^{3+} On The Fertilization-Induced Membrane Potential Changes In The Egg Of The Frog Rana Cameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Effects Of Cd^{2+}, Zn^{2+}, Li^+ And Al^{3+} On The Fertilization-Induced Membrane Potential Changes In The Egg Of The Frog Rana Cameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Turkish Journal of Biology
The apparent change in the egg membrane potential at fertilization gives rise to fertilization potential (FP). FP is the initial, transient electrical block to polyspermy as shown in most species especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until the permanent, mechanical block is set up. Polyspermy risk is increased when this effective electrical block cannot reach a certain peak value or when it is of short duration. On the other hand, metal accumulation in the external environment is potentially toxic to living beings, and we aimed in the present study to evaluate …
Restriction Fragment Length Polymorphism Analysis (Rflp) Of Some Streptomyces Strains From Soil, Ekrem Atalan
Restriction Fragment Length Polymorphism Analysis (Rflp) Of Some Streptomyces Strains From Soil, Ekrem Atalan
Turkish Journal of Biology
To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis. A dendrogram were constructed using the unweighed pair group method using arithmetic averages algorithm (UPGMA) after analysis restriction patterns. Five RFLP groups were obtained anone test strain was left as a single member group. RFLP profile indicated that unknown strains could be identified with data based on the interspacer region of …
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Biology
The promoter region of the §-glucanase gene was identified using a transcriptional fusion between the upstream region of the Streptococcus bovis \beta-glucanase gene and the celA gene. Using the transcriptional and protein localisation signals of the S. bovis \beta-glucanase gene, an in-frame tarnslational fusion between the end of the \beta-glucanase signal sequence and the ATG of the Neocallimastix patriciarum celA gene was constructed. The \beta-glucanase promoter-celA fusion was expressed in both E. coli and S. bovis. The activity of the protein produced was found to be cell-associated in E. coli, it but localised to the supernatant fraction of harvested cells …
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
Turkish Journal of Biology
Chromosomal polymorphism and speciation problem in genus Spalax has been investigated. All the specimens were identified as Spalax leucodon cilicicus Mehely, 1909. In according to karyotype analysis, two different populations were described from the field. The diploid number of Population A from south ant northwest of the field were found to be 60 and number of fundamental numbers were 80. The corresponding numbers in the Population B from northeast of the field were 54 and 74 respectively. In the result, the possibility of inversions in the Population B was discussed from evolutionary point of view and also the evolutionary background …
Dechlorination Of Bleached Kraft Pulp By Laccase Enzyme Produced From Some White-Rot Fungi, Nazi̇f Kolankaya, Arzu Taşpinar Ünal
Dechlorination Of Bleached Kraft Pulp By Laccase Enzyme Produced From Some White-Rot Fungi, Nazi̇f Kolankaya, Arzu Taşpinar Ünal
Turkish Journal of Biology
The possibility of using crude laccase in the dechlorination of chlorine-based bleached kraft pulp was investigated. Culture supernatants of seven white-rot fungal strains and kraft-pulp samples taken from E1 (Alkaline Extraction-1), E2 (Alkaline Extraction-2) and D2 (Chlorinedioxide treatment) stages of chlorine-based bleaching processes were used as the laccase source and substrates respectively during the studies. The dechlorination activity of Trametes versicolor was found to be more than that of the other fungi examined. The addition of a laccase inducer, xylidine, into the culture medium of T. Versicolor led to an increase in dechlorination activity. In the experiment performed to find …
Identification And Evaluation Of Clinically Significant Nocardia Brasiliensis, Nocardia Farcinica And Nocardia Otitidiscaviarum Strains Using Pyrolysis Mass Spectrometry (Pyms), Kami̇l Işik, Ergi̇n Kari̇ptaş, Nevzat Şahi̇n, Alan C. Ward, Micheal Goodfellow
Identification And Evaluation Of Clinically Significant Nocardia Brasiliensis, Nocardia Farcinica And Nocardia Otitidiscaviarum Strains Using Pyrolysis Mass Spectrometry (Pyms), Kami̇l Işik, Ergi̇n Kari̇ptaş, Nevzat Şahi̇n, Alan C. Ward, Micheal Goodfellow
Turkish Journal of Biology
Out of a total of thirty-nine Nocardia strains, eight species of N. brasiliensis, seventeen species of N. farcinica and fourteen species of N. otitidiscaviarum were identified using Pyrolysis mass spectrometry. N428, N477 N. brasiliensis; N669, N233 N. farcinica; and N231, N232 N. otitidiscaviarum duplicated strains were clustered in their own groups. Strains belonging to Nocardia brasiliensis, Nocardia farcinica and Nocardia otitidiscaviarum formed distinct pyrogroups corresponding to clusters defined in the numerical study. It can be concluded that PyMS provides a rapid and reproducible method of evaluating the taxonomic significance of Nocadia species.
Physiological Studies On The Plant Cell Wall Degrading Enzymes Of The Rumen Bacterium Ruminococcus Flavefaciens, Emi̇n Özköse, Mehmet Sai̇t Eki̇nci̇, Numan Özcan
Physiological Studies On The Plant Cell Wall Degrading Enzymes Of The Rumen Bacterium Ruminococcus Flavefaciens, Emi̇n Özköse, Mehmet Sai̇t Eki̇nci̇, Numan Özcan
Turkish Journal of Biology
Two forms of Ruminococcus flavefaciens, the wild type and a trained culture of strain 17 were compared for their cellulose and xylan degradative ability when then were grown in ryegrass, cellobiose and xylan for different incubation times. No cell-associated corboxymethylcellulase (CMCase) activity was observed after 1-day incubation in cellobiose, as no supernatant activity was detected after 1 and 3 days incubation in xylan and 2 days incubation in cellobiose grown culture. Both CMCase and xylanase activities of trained culture were higher than those of wild type culture when they were grown in ryegrass. Wild type cultures, however, showed higher levels …