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Articles 1 - 30 of 84
Full-Text Articles in Life Sciences
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Turkish Journal of Biology
An extracellular multiple form of endoxylanase was isolated from the xylanolytic complex of Aspergillus niger B03. The enzyme was purified to a homogenous form using ultrafiltration, anion exchange chromatography, and gel filtration. It was a nonglycosylated protein with a molecular weight of 20,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 21,000 Da as determined by gel filtration. The optimal pH for the enzyme action was 5.0 and the optimal temperature was 55 °C. Endoxylanase stability was significantly improved in the presence of glycerol and sorbitol. The enzyme activity was activated by Mn^{2+} and Co^{2+}, and it was …
Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r
Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r
Turkish Journal of Biology
Lipase production was examined with 3 strains of Yarrowia lipolytica: NBRC 1658, IFO 1195, and a local strain. All strains were obtained from Hacettepe University's Department of Food Engineering. The addition of nitrogen and carbon sources and the effects of initial pH and temperature parameters were studied. For all 3 strains, the growth temperature range was 20-40 °C, and maximum growth was determined at 30 °C. The initial pH range was 6-9 for growth and 3-6 for lipase production. Maximum growth results were detected as follows: Y. lipolytica NBRC 1658 at 30 °C and pH 8, Y. lipolytica IFO 1195 …
Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli
Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli
Turkish Journal of Biology
GJB2 encodes connexin 26, a gap junction protein that is assumed to be a component of the potassium recycling pathway in the inner ear. Loss or malfunction of these gap junctions, as might be reflected by mutations in GJB2, may disrupt potassium movement from the hair cells through the supporting cell network to the endolymph, leading to hearing impairment. One mutation, the deletion of 1 guanosine residue from a stretch of 6 between nucleotide positions 30 and 35 (35delG) at codon 10, is the most common deafness-causing allelic variant of GJB2 in sporadic patients and autosomal recessive families. Mutations in …
Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala
Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala
Turkish Journal of Biology
Low phosphate solubility is one of the most important factors limiting the plant growth in Indian soils. Many microorganisms can enhance phosphate solubility, but little is known about the magnitude of their phosphorus-solubilizing ability. The native populations of phosphate-solubilizing bacteria and fungi were studied in different rhizospheric soil samples obtained from betel vine plants (Piper betel L.) in order to compare the results. The present study focuses on the phosphate-solubilizing capacity of bacteria and fungi in rhizospheric soil samples obtained from betel vine plants, revealing the dominance of Aspergillus species (26.1 mm) as major phosphate solubilizers, along with Bacillus subtilis …
Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer
Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer
Turkish Journal of Biology
Salvia L. species have been used for the treatment of various inflammatory ailments in traditional medicine. In order to evaluate this ethnobotanical information, water, methanol, n-butanol, acetone, and chloroform extracts from 3 Salvia species (S. fruticosa, S. verticillata, and S. trichoclada) were screened for their anti-inflammatory activity using in vivo experimental models in rats. For this purpose a carrageenan-induced inflammatory paw edema model was used. All extracts demonstrated anti-inflammatory activities; however, n-butanol extract of Salvia fruticosa (syn. S. triloba), which is known as Turkish sage, was found to be the most active. It can be expected that the active flavonoids, …
Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n
Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n
Turkish Journal of Biology
Beta-carotene and silymarin have antioxidant properties against oxidative damage and are used as dietary supplements. The aim of this study was to assess the protective effects of \beta-carotene and silymarin on healthy human lymphocytes exposed to L-arginine-induced oxidative damage. Study samples were lymphocyte cultures set up from venous blood obtained from 6 healthy individuals (3 males and 3 females). Oxidative DNA damage was induced by L-arginine. \beta-Carotene and silymarin were added to the cultures as antioxidants to observe their protective effects, if any. Alkaline single-cell gel electrophoresis (SCGE) was used to evaluate DNA damage. While L-arginine generated genotoxic damage in …
Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton
Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton
Turkish Journal of Biology
The villages of Ilıca District in Erzurum Province, Turkey, were surveyed in terms of plants and animals used traditionally for medicinal purposes. Plants were compiled according to their families, botanical names, local names, usages, and applications. A total of 70 plant species belonging to 29 families are used by village people to treat a wide range of complaints, such as hemorrhoids, urinary system infections, stomach disorders, respiratory diseases, skin diseases, rheumatism, and jaundice. In addition, 3 animal species are used for the treatment of some diseases. The collected data were compared with those formerly recorded.
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Turkish Journal of Biology
Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diffusion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at IC_{50} = 85 ± 21.7 µg/mL. The best incubation time …
Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek
Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek
Turkish Journal of Biology
Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and \alpha-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential …
Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er
Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er
Turkish Journal of Biology
The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts (including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate) on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract …
Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk
Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk
Turkish Journal of Biology
Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the …
Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt
Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt
Turkish Journal of Biology
The present study was carried out to determine the changes in some reserve molecules of safflower seeds during germination and early seedling growth. The seeds of 2 safflower cultivars were used as plant material (Dinçer 5-118 and Montola 2000). Seeds were germinated for 72 h and observations were taken every 12 h throughout the study. Changes in total oil content (%), total protein content (%), total soluble sugar contents (TSS, mg g^{-1}), and reducing sugar contents (RS, mg g^{-1}) were determined for each cultivar during germination and seedling growth. The oil content of the cultivars decreased from 54.7% and 57.4% …
The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun
The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun
Turkish Journal of Biology
Growth parameters and the reproduction period of white bream (Blicca bjoerkna L. 1758) sampled from Sapanca Lake were determined monthly between November 2000 and October 2001. Three hundred and fifty white bream specimens caught ranged in age from 1^+ to 9^+ years, and the majority of the samples consisted of individuals of the 5^+-year-old age group. The von Bertalanffy equation was determined as Lt = 22.169 (1 - exp {-0.215[t + 0.986]}), Ø': 2.02 for males and Lt = 31.917 (1 - exp {-0.122[t + 1.087]}), Ø': 2.10 for females. The length-weight relationship was estimated as W = 0.0046 TL …
The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar
The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar
Turkish Journal of Biology
The effects of seasonal variation on the fatty acid composition of total lipid, triacylglycerol, and phospholipid in the dorsal muscle of Capoeta trutta were assessed by gas chromatographic method. A total of 18 different fatty acids were determined in the composition of C. trutta. The variations in fatty acid composition of total lipid, triacylglycerol, and phospholipid were recorded throughout the seasons. The total polyunsaturated fatty acids (PUFA) and monounsaturated fatty acids (MUFA) were the most important groups of total lipid. Total saturated fatty acid (SFA) and total MUFA were seen to increase from spring to summer while total PUFA decreased …
The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal
The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal
Turkish Journal of Biology
This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) alpha, pi, mu, and theta in prostatic acinar adenocarcinoma (PCA), prostatic intraepithelial neoplasia (PIN), and benign prostatic tissues from 19 patients. Relationships between GST isoenzyme expression in benign, PIN, and PCA tissue were examined by the Wilcoxon signed-rank test and clinicopathological data were examined by the Spearman correlation rank test. When the benign, PIN, and PCA tissues from these cases were compared according to their staining intensity, GST alpha, pi, mu, and theta expressions in tumor cells were significantly lower than in benign epithelial cells (P < 0.05). The GST alpha class displayed the lowest level of expression in PIN and PCA. Expression of GST pi was lower in PCA tissue than in PIN and benign epithelial tissue (P < 0.05). We hypothesize that carcinogenesis in the prostate results from impaired cellular handling of mutagenic agents owing to reduction or loss of expression of multiple GST isoenzymes and other detoxifying and antimutagenesis agents. This study confirms the down-regulation of GST isoenzymes in PCA of the prostate and shows that the loss of GST isoenzyme expression is a phenotype associated with malignant transformation. There was no statistical relationship between GST isoenzyme expression and the clinicopathological data (age, Gleason score, and total serum prostate-specific antigen levels) (P > 0.05).
Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Turkish Journal of Biology
Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants …
Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu
Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu
Turkish Journal of Biology
Oral squamous cell carcinomas (OSCCs) are the most common type of oral cancers, and high morbidity and mortality are largely attributable to late-stage diagnosis. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl trisulfide (DATS), an oil-soluble organosulfur compound of garlic, was recently reported for its anticancer effect on several types of cancer cells in vitro; however, these anticancer effects are still unknown in human OSCC cells. In the present study, the effects of DATS on OSCC cell invasion in vitro were investigated. Human OSCC cell lines (HSC-2 and Ca9-22 cells) were treated with DATS …
Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol
Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol
Turkish Journal of Biology
Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus …
Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade
Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade
Turkish Journal of Biology
Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class …
Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti
Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti
Turkish Journal of Biology
Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 µg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were grown on direct mushroom bed extract, 0.2% glucose-containing mushroom bed extract, and common production medium for determination of growth and lignolytic activities. Since lignolytic activities were high with the direct mushroom bed extract, this medium was further diluted to 1:1 and 1:2 ratios and …
Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk
Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk
Turkish Journal of Biology
Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia (At-ext) were investigated using both Allium cepa root meristematic cells and a short-term mutation assay in Salmonella typhimurium with TA98 and TA100 strains in the presence or absence of S9 mix, respectively. In the Allium tests, EC_{50} value was determined as 50 g/L, and 0.5 × EC_{50}, EC_{50}, and 2 × EC_{50} concentrations of At-ext were applied to onion tuber roots. Methyl methane sulfonate (MMS, 10 ppm) and distilled water were used as positive and negative controls, respectively. The mitotic index (MI) increased in all concentrations in …
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Turkish Journal of Biology
Phoenix dactylifera L. was introduced in Tunisia a very long time ago and plays an important socioeconomic role, especially in the south of the country. Genetic diversity and relationships among 26 cultivars were assessed with 7 ISSR primers. A total of 43 amplified bands were obtained. Principal component analyses based on Nei genetic distances showed no geographical separation with the exception of the Dhahbi cultivar, which has a limited geographical distribution. A group of cultivars that are also phonetically clustered was distinguished. These cultivars have a common maturity period and a common fruit consistency. The Mantel test emphasizes a significant …
Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva
Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva
Turkish Journal of Biology
One of the natural reservoirs of potentially human-pathogenic bacteria is believed to be the rhizosphere. The aim of the present work was to test nontuberculous mycobacterium Mycobacterium phlei MbP18 for its ability to colonize the rhizosphere of wheat and to evaluate its effect on plant growth under saline conditions. In competitive wheat root tip colonization assays, M. phlei MbP18 showed poor competitive colonization of the wheat rhizosphere compared to the reference strain. The strain produced lipase, amylase, cellulase, and pectinase and grew well in the presence of high salt (up to 4% NaCl) and at high temperatures (up to 40 …
Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy
Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy
Turkish Journal of Biology
Oxidative stress responses were evaluated in the digestive gland tissue of Perna viridis exposed to water-accommodated fractions (WAFs) of petrol and diesel at 0.5% and 5% concentrations over 5-, 10-, and 15-day periods. Increased lipid peroxidation, protein carbonyl, DNA integrity, and hydrogen peroxide in WAF-exposed specimens are indicative of oxidative damage due to dissolved petroleum extracts. A consistent increase in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione, and ascorbic acid in WAF-exposed specimens signify the importance of antioxidants in protecting the cell against the oxidative damage arising from petroleum contamination. The responses of field specimens collected from …
Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin
Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin
Turkish Journal of Biology
Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that …
Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul
Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul
Turkish Journal of Biology
The genus Fritillaria embraces about 165 taxa in the family Liliaceae. In this study, the molecular phylogenetic relationships between 10 Fritillaria taxa were presented. Fritillaria spp. were collected from their natural habitats, and specimens with different morphological features were obtained via selective breeding. Specimens with the desired characters were presented as promising candidate cultivars for ornamental use. Phylogenetic analysis was based on DNA sequences of the chloroplast trnL-trnF region. The phylogeny was constructed using the neighbor joining, maximum parsimony, maximum likelihood, and Bayesian inference methods. The data showed that the examined Fritillaria spp. were evidently diverged into 2 Fritillaria subgenera. …
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin
Turkish Journal of Biology
Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.
Evaluation Of Biological Activities Of Goldmoss Stonecrop (Sedum Acre L.), Milan Stankovic, Ivana Radojevic, Milena Curcic, Sava Vasic, Marina Topuzovic, Ljiljana Comic, Snezana Markovic
Evaluation Of Biological Activities Of Goldmoss Stonecrop (Sedum Acre L.), Milan Stankovic, Ivana Radojevic, Milena Curcic, Sava Vasic, Marina Topuzovic, Ljiljana Comic, Snezana Markovic
Turkish Journal of Biology
In vitro antioxidant, antimicrobial, and antiproliferative activities of methanol, acetone, and ethyl acetate extracts of Sedum acre L. were determined. Their total phenolic contents as well as the concentrations of flavonoids were also evaluated. The total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 18.25 to 181.75 mg gallic acid/g. The concentration of flavonoids in various extracts of S. acre was determined by using a spectrophotometric method with aluminum chloride and the obtained results varied from 8.42 to 173.42 mg rutin/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC_{50} (\mug/mL), and its values ranged …
Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova
Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova
Turkish Journal of Biology
The antioxidant and acetylcholinesterase inhibitory potential of methanol extract from Arnica montana cultivated in Bulgaria was evaluated. For the determination of antioxidant activity 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) free radicals, and ferric reducing antioxidant power (FRAP) assay were used. Modified Ellman's colorimetric method was used for quantitative assessment of acetylcholinesterase inhibition potential. Butylated hydroxytoluene (BHT) and galantamine hydrobromide were used as positive controls, respectively. In addition, total polyphenols and tannins, flavonoids, hydroxycinnamic derivatives, and sesquiterpene lactones were determined using Folin-Ciocalteu reagent, AlCl_3, Na_2MoO_4, and m-dinitrobenzene, respectively. The results demonstrated that Arnica extract has strong antioxidant activity and moderate …
A Comparison Of Major Taste- And Health-Related Compounds Of Vaccinium Berries, Jasminka Milivojevic, Vuk Maksimovic, Jelena Dragisic Maksimovic, Dragan Radivojevic, Milena Poledica, Sezai̇ Erci̇şli̇
A Comparison Of Major Taste- And Health-Related Compounds Of Vaccinium Berries, Jasminka Milivojevic, Vuk Maksimovic, Jelena Dragisic Maksimovic, Dragan Radivojevic, Milena Poledica, Sezai̇ Erci̇şli̇
Turkish Journal of Biology
The chemical fruit composition, phenolic profile, and corresponding total antioxidant activity of bilberry (Vaccinium myrtillus) and 2 commercial blueberry cultivars (Vaccinium corymbosum) were analyzed. Results from this study showed that cultivar Berkeley yielded the highest glucose and fructose contents (70.8 and 88.8 mg/g FW, respectively), and the sweetness index expressed a similar trend, achieving the highest value for cultivar Berkeley (279.2). Citric acid was the major organic acid in the berries tested. V. myrtillus yielded a total organic acids value 2-fold higher (0.35 mg/g FW) as well as the highest vitamin C content (25.8 mg/100 g FW). Among the flavonols …