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Nucjuke: A Web Tool For Re-Ranking Crispr-Cas9 Grnas Based On Chromatin Accessibility In Yeast, Gaea Turman
Nucjuke: A Web Tool For Re-Ranking Crispr-Cas9 Grnas Based On Chromatin Accessibility In Yeast, Gaea Turman
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A variety of papers published in the last decade have suggested that chromatin accessibility could have significant influence over the success of CRISPR-Cas9 experiments in S. cerevisiae; where chromatin accessibility refers to nucleosome presence at a targeted genomic location. It has been found that nucleosome presence can physically impede Cas9 from making a double stranded break at a specific target site, causing a decreased experimental efficiency. We have created a web-tool called NucJuke that seeks to mitigate this problem by categorically re-ranking gRNAs based on having high, partial, or low chromatin accessibility (referring to no nucleosome occupancy, partial nucleosome occupancy, …