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Full-Text Articles in Life Sciences
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Journal of the South Carolina Academy of Science
No abstract provided.
Targeted Insertion Of The Mping Transposable Element, Ashley E. Strother, Stephanie S. Diaz, Mary E. Baker, C. Nathan Hancock
Targeted Insertion Of The Mping Transposable Element, Ashley E. Strother, Stephanie S. Diaz, Mary E. Baker, C. Nathan Hancock
Journal of the South Carolina Academy of Science
Class II DNA Transposable Elements (TEs) are moved from one location to another in the genome by the action of transposase proteins that bind to repeat sequences at the ends of the elements. Although the location TE insertion is mostly random, the addition of DNA binding domains to the transposase proteins has allowed for targeted insertion of some elements. In this study, the Gal4 binding domain was added to the transposase proteins, ORF1 and TPase, which mobilize the mPing element from rice. The Gal4:TPase construct was capable of increasing the number of mPing insertions into the Gal2 and Gal4 promoter …
Determining The First Intermediate Host To Parasites Glossocercus Caribaensis And Cyclustera Ibisae That Are Found In Fundulus Heteroclitus In The North Inlet-Winyah Bay National Estuarine Research Reserve, Emiley Masloski, Marlee B. Marsh
Determining The First Intermediate Host To Parasites Glossocercus Caribaensis And Cyclustera Ibisae That Are Found In Fundulus Heteroclitus In The North Inlet-Winyah Bay National Estuarine Research Reserve, Emiley Masloski, Marlee B. Marsh
Journal of the South Carolina Academy of Science
Fundulus heteroclitus serves as a host to many parasites, providing a model system to investigate questions regarding evolution and ecology of host-parasite interactions in natural populations. Previous research from the North Inlet Estuary at Belle W. Baruch shows that F. heteroclitus serve as second intermediate hosts to cestodes, Glossocercus caribaensis and Cyclustera ibisae. Finding larval parasites in the first intermediate host will complete gaps in the knowledge about these parasites’ life cycles. In the experiment, 27 fish were collected from the North Inlet Estuary and were dissected and examined for parasites around the gastrointestinal tract. Parasites collected were sent …
Identification Of Coliphages In The Aviary At Brookgreen Gardens And The Factors That Might Influence Coliphage Population Dynamics In This Cypress Swamp Environment, Olivia Shirley, Lisa Pieterse, Paul E. Richardson
Identification Of Coliphages In The Aviary At Brookgreen Gardens And The Factors That Might Influence Coliphage Population Dynamics In This Cypress Swamp Environment, Olivia Shirley, Lisa Pieterse, Paul E. Richardson
Journal of the South Carolina Academy of Science
It has been understood that the presence of coliphages (bacteriophages specific for E. coli) in water is an indirect measurement of fecal contamination. Bacteriophages have been suggested to hold an important role in regulating the bacterial population. This pilot study analyzes the relationship between the presence of coliphages in the water in the aviary at Brookgreen Gardens and how environmental factors such as temperature, pH, and turbidity affect the presence of these viruses over the time period of three months (June 2017 through August 2017). Coliphages found in volary water sources were speculated to be deposited directly by birds that …
Investigating The Lytic Staphylococcus Aureus Bacteriophage Reservoir Amongst A South Carolina University Population: Discovery, Characterization, And Identification Of A Potential Bacteriophage Treatment For Methicillin-Resistant Staphylococcus Aureus, Lisa Pieterse, Amy Powers, Derek Pride, Lisha Van Onselen, Giovanna E. Leone, Paul E. Richardson
Investigating The Lytic Staphylococcus Aureus Bacteriophage Reservoir Amongst A South Carolina University Population: Discovery, Characterization, And Identification Of A Potential Bacteriophage Treatment For Methicillin-Resistant Staphylococcus Aureus, Lisa Pieterse, Amy Powers, Derek Pride, Lisha Van Onselen, Giovanna E. Leone, Paul E. Richardson
Journal of the South Carolina Academy of Science
Bacteriophages are viruses that only infect bacterial cells and can be used to treat antibiotic resistant bacterial infections. This study focused on the isolation and characterization of bacteriophages lytic to Staphylococcus aureus at Coastal Carolina University (CCU) in Conway, South Carolina, as a means to isolate bacteriophages that can potentially be used to treat methicillin-resistant Staphylococcus aureus (MRSA), an antibiotic-resistant S. aureus variant. From 2014 to 2018, collection of ear and nose samples from 225 randomly selected CCU volunteers was conducted. Filter sterilization, amplification, microbial tests, and PCR analyses were performed in order to identify and characterize bacteriophages. Coliphage populations …
An Investigation Of The Order Of Applying An Emollient With A Topical Steroid In The Treatment Of Atopic Dermatitis, Andrea Conner, Ashlee Tietje
An Investigation Of The Order Of Applying An Emollient With A Topical Steroid In The Treatment Of Atopic Dermatitis, Andrea Conner, Ashlee Tietje
Journal of the South Carolina Academy of Science
In 2007, the prevalence of Atopic Dermatitis had increased two to three-fold within the last three decades and affected 15-20% of young children (Buys, 2007). Current treatment includes the use of both steroid and emollient creams. Current suggestions for the order of application are contradictory. This study aims to examine the role of the order of application of treatments for Atopic Dermatitis (AD). Hairless mice (SKH-1) were induced to a mild AD flare-up using 2,4-dinitrochlorobenzene (DNCB) and treated with either 1% hydrocortisone (Maximum Strength Cortizone 10 cream) alone, 1% hydrocortisone followed by Cetaphil (emollient), or Cetaphil followed by 1% hydrocortisone. …
Table Of Contents
Journal of the South Carolina Academy of Science
No abstract provided.
Nine-Banded Armadillo (Dasypus Novemcinctus) In Spartanburg County, South Carolina, Jonathan J. Storm
Nine-Banded Armadillo (Dasypus Novemcinctus) In Spartanburg County, South Carolina, Jonathan J. Storm
Journal of the South Carolina Academy of Science
The distribution range of the nine-banded armadillo (Dasypus novemcinctus) has been expanding northward across the eastern and central United States over the past several decades. It is thought that armadillos first reached South Carolina in the mid-1980s in the southwestern portion of the state. Armadillo sightings are rare in the Upstate region of South Carolina. Here, we report of an armadillo photographed in southern Spartanburg County on 30 May, 2017. This individual was spotted in mixed deciduous forest along the Tyger River. Our data add further support to the notion that armadillos have expanded their breeding range into …
Note On Suspected Brown Recluse Spiders (Araneae: Sicariidae) In South Carolina, Robert J. Wolff
Note On Suspected Brown Recluse Spiders (Araneae: Sicariidae) In South Carolina, Robert J. Wolff
Journal of the South Carolina Academy of Science
No abstract provided.
Building Soft Nanomachines From Polydiacetylene Liposomes, Timothy W. Hanks
Building Soft Nanomachines From Polydiacetylene Liposomes, Timothy W. Hanks
Journal of the South Carolina Academy of Science
No abstract provided.