Life Sciences Commons^™

Synthesis And In Vitro Antimycobacterial Activity Of Novel N-Arylpiperazines Containing An Ethane-1,2-Diyl Connecting Chain, Tomás Gonec, Ivan Malík, Jozef Csöllei, Josef Jampílek, Jirina Stolaríková, Ivan Solovic, Peter Miku, Stanislava Keltoová, Peter Kollár, Jim O'Mahony, Aidan Coffey

Department of Biological Sciences Publications

© 2017 by the authors. Novel 1-(2-{3-/4-[(alkoxycarbonyl)amino]phenyl}-2-hydroxyethyl)-4-(2-fluorophenyl)-piperazin-1-ium chlorides (alkoxy = methoxy to butoxy; 8a-h) have been designed and synthesized through multistep reactions as a part of on-going research programme focused on finding new antimycobacterials. Lipophilic properties of these compounds were estimated by RP-HPLC using methanol/water mobile phases with a various volume fraction of the organic modifier. The log kw values, which were extrapolated from intercepts of a linear relationship between the logarithm of a retention factor k (log k) and volume fraction of a mobile phase modifier (jM), varied from 2.113 (compound 8e) to 2.930 (8h) and indicated relatively high …

Emergence Of The L Phenotype In Group B Streptococci In The South Of Ireland, Katherine Hayes, Lesley Cotter, L. Barry, Fiona O'Halloran

Department of Biological Sciences Publications

Group B Streptococcal isolates (n = 235) from the South of Ireland were characterised by serotyping, antimicrobial susceptibility and determination of the phenotypic and genotypic mechanisms of resistance. Resistance to erythromycin and clindamycin was observed in 21·3% and 20·4% of the total population, respectively. The c-MLSB phenotype was the most common phenotype detected (62%), with ermB being the predominant genetic determinant, present in 84% of resistant isolates. The rare L phenotype was observed in 2·9% (n = 7) of isolates, four of which harboured the lsaC gene responsible for clindamycin resistance. Serotypes Ia, III and II were the most common …

Is Tb Testing Associated With Increased Blood Interferon-Gamma Levels?, Aideen E. Kennedy, Jim O'Mahony, Noel Byrne, John Macsharry, Riona G. Sayers

Department of Biological Sciences Publications

The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 …

Draft Genome Sequences Of 25 Listeria Monocytogenes Isolates Associated With Human Clinical Listeriosis In Ireland, Eamonn P. Culligan, Et. Al.

Department of Biological Sciences Publications

Listeria monocytogenes is a Gram-positive opportunistic pathogen that is the causative agent of listeriosis. Here, we report the draft genome sequences of 25 L. monocytogenes strains isolated from patients with clinical listeriosis in the Republic of Ireland between 2013 and 2015.

Genome Sequence Of Staphylococcus Saprophyticus Dpc5671, A Strain Isolated From Cheddar Cheese, Caitríona M. Guinane, Et. Al.

Department of Biological Sciences Publications

The draft genome sequence of Staphylococcus saprophyticus DPC5671, isolated from cheddar cheese, was determined. S. saprophyticus is a common Gram-positive bacterium detected on the surface of smear-ripened cheese and other fermented foods.

GENOME ANNOUNCEMENT

A Blueprint To Evaluate One Health, Simon R. Rüegg, Barry J. Mcmahon, Barbara Häsler, Roberto Esposito, Helen O'Shea, Et Al

Department of Biological Sciences Publications

One Health (OH) positions health professionals as agents for change and provides a platform to manage determinants of health that are often not comprehensively captured in medicine or public health alone. However, due to the organization of societies and disciplines, and the sectoral allocation of resources, the development of transdisciplinary approaches requires effort and perseverance. Therefore, there is a need to provide evidence on the added value of OH for governments, researchers, funding bodies, and stakeholders. This paper outlines a conceptual framework of what OH approaches can encompass and the added values they can provide. The framework was developed during …

The Association Of Serum Levels Of Brain-Derived Neurotrophic Factor With The Occurrence Of And Recovery From Delirium In Older Medical Inpatients, John Williams, Karen Finn, Vincent Melvin, David Meagher, Geraldine Mccarthy, Dimitrios Adamis

Department of Biological Sciences Publications

Limited studies of the association between BDNF levels and delirium have given inconclusive results. This prospective, longitudinal study examined the relationship between BDNF levels and the occurrence of and recovery from delirium. Participants were assessed twice weekly using MoCA, DRS-R98, and APACHE II scales. BDNF levels were estimated using an ELISA method. Delirium was defined with DRS-R98 (score > 16) and recovery from delirium as ≥2 consecutive assessments without delirium prior to discharge. We identified no difference in BDNF levels between those with and without delirium. Excluding those who never developed delirium (), we examined the association of BDNF levels and …

Things Are Getting Hairy: Enterobacteria Bacteriophage Vb_Pcam_Cbb, Colin Buttimer, Hanne Hendrix, Hugo Oliveira, Aidan Casey, Horst Neve, Olivia Mcauliffe, R. Paul Ross, Colin Hill, Jean Paul Noben, Jim O'Mahony, Rob Lavigne, Aidan Coffey

Department of Biological Sciences Publications

© 2017 Buttimer, Hendrix, Oliveira, Casey, Neve, McAuliffe, Ross, Hill, Noben, O'Mahony, Lavigne and Coffey. Enterobacteria phage vB_PcaM_CBB is a "jumbo" phage belonging to the family Myoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the genera Erwinia, Pectobacterium, and Cronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis …