Life Sciences Commons^™

Diversity And Substrate-Specificity Of Green Algae And Other Micro-Eukaryotes Colonizing Amphibian Clutches In Germany, Revealed By Dna Metabarcoding, Sten Anslan, Maria Sachs, Lois Rancilhac, Henner Brinkmann, Jörn Petersen, Sven Künzel, Anja Schwarz, Hartmut Arndt, Ryan R. Kerney, Miguel Vences

Biology Faculty Publications

Amphibian clutches are colonized by diverse but poorly studied communities of micro-organisms. One of the most noted ones is the unicellular green alga, Oophila amblystomatis, but the occurrence and role of other micro-organisms in the capsular chamber surrounding amphibian clutches have remained largely unstudied. Here, we undertook a multi-marker DNA metabarcoding study to characterize the community of algae and other micro-eukaryotes associated with agile frog (Rana dalmatina) clutches. Samplings were performed at three small ponds in Germany, from four substrates: water, sediment, tree leaves from the bottom of the pond, and R. dalmatina clutches. Sampling substrate strongly …

A Linkage Map For The Newt Notophthalmus Viridescens: Insights In Vertebrate Genome And Chromosome Evolution, Melissa C. Keinath, S. Randal Voss, Panagiotis A. Tsonis, Jeramiah J. Smith

Biology Faculty Publications

Genetic linkage maps are fundamental resources that enable diverse genetic and genomic approaches, including quantitative trait locus (QTL) analyses and comparative studies of genome evolution. It is straightforward to build linkage maps for species that are amenable to laboratory culture and genetic crossing designs, and that have relatively small genomes and few chromosomes. It is more difficult to generate linkage maps for species that do not meet these criteria. Here, we introduce a method to rapidly build linkage maps for salamanders, which are known for their enormous genome sizes. As proof of principle, we developed a linkage map with thousands …

Review Of "Eye Of Newt And Toe Of Frog, Adder’S Fork And Lizards’ Leg, The Lore And Mythology Of Amphibians And Reptiles", Kay Etheridge

Biology Faculty Publications

A review of Marty Crump's book on the folklore surrounding reptiles and amphibians. Crump's book is a collection of tales and myths both ancient and contemporary, and a fascinating analysis of how humans perceive and sometimes revere snakes, frogs and other "loathsome" creatures.

An Integrative Approach To Phylogeography: Investigating The Effects Of Ancient Seaways, Climate, And Historical Geology On Multi-Locus Phylogeographic Boundaries Of The Arboreal Salamander (Aneides Lugubris), S B. Reilly, Ammon Corl, D B. Wake

Biology Faculty Publications

Background: Phylogeography is an important tool that can be used to reveal cryptic biodiversity and to better understand the processes that promote lineage diversification. We studied the phylogeographic history of the Arboreal Salamander (Aneides lugubris), a wide-ranging species endemic to the California floristic province. We used multi-locus data to reconstruct the evolutionary history of A. lugubris and to discover the geographic location of major genetic breaks within the species. We also used species distribution modeling and comparative phylogeography to better understand the environmental factors that have shaped the genetic history of A. lugubris.

Results: We found six major mitochondrial clades …

Phylogenetic Analysis Of Algal Symbionts Associated With Four North American Amphibian Egg Masses, Eunsoo Kim, Yuan Lin, Ryan R. Kerney, Lili Blumenberg, Cory Bishop

Biology Faculty Publications

Egg masses of the yellow-spotted salamander Ambystoma maculatum form an association with the green alga “Oophila amblystomatis” (Lambert ex Wille), which, in addition to growing within individual egg capsules, has recently been reported to invade embryonic tissues and cells. The binomial O. amblystomatis refers to the algae that occur in A. maculatum egg capsules, but it is unknown whether this population of symbionts constitutes one or several different algal taxa. Moreover, it is unknown whether egg masses across the geographic range of A. maculatum, or other amphibians, associate with one or multiple algal taxa. To address these …

A New Species Of Chiasmocleis (Microhylidae, Gastrophryninae) From The Atlantic Forest Of Espírito Santo State, Brazil, João Filipe Tonini, Maruicio Forlani, Rafael O. De Sá

Biology Faculty Publications

Among Neotropical microhylids, the genus Chiasmocleis is exceptionally diverse. Most species ofChiasmocleis were described in recent years based on external morphology, but recent studies using molecular data did not support the monophyly of the species groups clustered based on feet webbing. Furthermore, a phylogeographic study of C. lacrimae estimated high genetic divergence and low gene flow among populations across small geographic ranges. Increasing the molecular and geographic sampling, and incorporating morphological data, we identified new cryptic species. Herein, we used novel genetic and morphological data to describe a new species of Chiasmocleis.

Long-Term Data For Endemic Frog Genera Reveal Potential Conservation Crisis In The Bale Mountains, Ethiopia, David J. Gower, Roman K. Aberra, Silvia Schwaller, Malcolm J. Largen, Ben Collen, Stephen Spawls, Michele Menegon, Breda M. Zimkus, Rafael O. De Sá, Et Al.

Biology Faculty Publications

Populations of many frogs have declined alarmingly in recent years, placing nearly one third of the > 6,000 species under threat of extinction. Declines have been attributed largely to habitat loss, environmental degradation and/or infectious diseases such as chytridiomycosis. Many frogs undergo dramatic natural population fluctuations such that long-term data are required to determine population trends without undue influence of stochastic factors. We present long-term quantitative data (individuals encountered per person hour of searching) for four monotypic frog genera endemic to an Afromontane region of exceptional importance but growing conservation concern: one endemic to the Ethiopian highlands (Spinophrynoides osgoodi) and three …

Leptodactylus Syphax, W. R. Heyer, Miriam M. Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus syphax are moderately sized (males 58-83 mm, females 70-90 mm SVL). The head is about as long as wide, but usually is just wider than long. The hind limbs are moderately short (Table 1; Heyar and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are laterally expanded, tan, and not darker than the adjacent throat. The male snout is not spatulate, the snout profile is rounded to obtuse in both sexes. Male arms are hypertrophied during the breeding season in sexually active males. The male thumb has two large, …

Is The Amphibian Tree Of Life Really Fatally Flawed?, Darrel R. Frost, Taran Grant, Julian Faivovich, Raoul H. Bain, Alexander Haas, Celio F.B. Haddad, Rafael O. De Sá, Et Al.

Biology Faculty Publications

Wiens (2007, Q. Rev. Biol. 82, 55–56) recently published a severe critique of Frost et al.'s (2006, Bull. Am. Mus. Nat. Hist. 297, 1–370) monographic study of amphibian systematics, concluding that it is “a disaster” and recommending that readers “simply ignore this study”. Beyond the hyperbole, Wiens raised four general objections that he regarded as “fatal flaws”: (1) the sampling design was insufficient for the generic changes made and taxonomic changes were made without including all type species; (2) the nuclear gene most commonly used in amphibian phylogenetics, RAG-1, was not included, nor were the morphological characters that had justified …

Regeneration Of Retinotectal Projections After Optic Tectum Removal In Adult Newts, Mitsumasa Okamoto, Hatsuki Ohsawa, Toshinori Hayashi, Katsushi Owaribe, Panagiotis A. Tsonis

Biology Faculty Publications

Purpose: When injured, the adult newt possesses the remarkable capability to regenerate tissues and organs with return of function and physiology. One example is the newt eye, in which regeneration can restore normal vision if the retina or lens has been removed. We wanted to examine how the retinotectal projections regenerate after removal of the brain’s optic tectum and establish this animal as a model for retinal projection as well as a central nervous system regeneration model.

Loathsome Beasts: Images Of Reptiles And Amphibians In Art And Science, Kay Etheridge

Biology Faculty Publications

The mythology and symbolism historically associated with reptiles and amphibians is unequaled by that of any other taxonomic group of animals. Even today, these creatures serve as icons - often indicating magic or evil - in a variety of media. Reptiles and amphibians also differ from other vertebrates (i.e. fish, mammals and birds) in that most have never been valued in Europe as food or for sport. Aside from some limited medicinal uses and the medical concerns related to venomous species, there was little utilitarian value in studying the natural history of reptiles and amphibians. Because of this history and …

Distribución Geográfica De La Fauna De Anfibios Del Uruguay, Diego Nuñez, Raúl Maneyro, José A. Langone, Rafael O. De Sá

Biology Faculty Publications

Datos precises sobre la distribución geográfica de los organismos de una región son necesarios y prerrequisito para entender las interrelaciones ecológicas entre las comunidades biológicas. Esta información también contribuye a entender el componente histórico de la región posibilitando la determinación de centros de origen y el establecimiento de patrones de diversidad (Ron, 2000), así mismo posibilita realizar inferencias acerca de los mecanismos de especiación en los diferentes grupos zoológicos (Bridarolli & Di Tada, 1994).

La distribución geográfica de los anfibios en Uruguay ha sido parcialmente reportada (Achaval & Olmos, 1997; Langone, 1995; Maneyro et al, 1995; Maneyro & Langone, 2001). …

Los Anfibios Del Monumento Natural Barro Colorado, Parque Nacional Soberania Y Areas Adyacentes, Rafael O. De Sá

Biology Faculty Publications

This book focuses on the batrachofauna of Barro Colorado Nature Monument. Soberania National Park and adjacent areas. It complements other recent publications on Neotropical amphibians such as those by Rodriguez and Duellman ( 1994) on Peru, Achaval and Olmos (1995) on Uruguay, and Meyer and Foster (1996) on Belize. The goal of these books is to reach a wider audience than the more technical batrachofaunal summaries-e.g., Frogs of Boracéia (Heyer 1990). Amphibians of Argentina (Cei 1985), and Frogs of the Genus Eleutherodactylus in Western Ecuador (Lynch and Duellman 1997). These geographically focused guides are especially useful to students, amateur biologists, …

Hyla Multifasciata, Rafael O. De Sá

Biology Faculty Publications

Hyla multifasciata is a medium to large species in the albopunctata species group, adults ranging in size from 42 - 61 mm SVL (mean male SVL = 48 mm, mean female SVL = 53 mm). The head is longer than wide. The snout is rounded in dorsal view, whereas in lateral view it is rounded and protrudes slightly beyond the mandible. The canthus rostralis is rounded and distinct, the loreal region is concave, and the internarial region is convex. The eyes are large, approximately twice the diameter of the tympanum. The tympanum is separated from the eye by a distance …

Morfología Y Funciones Del Conducto De Müller De Chthonerpeton Indistinctum (Reinhardt Y Lutken, 1862) (Amphibia: Gymnophiona) En Relación Con Los Mecanismos Reproductivos De La Especie, Nibia Berois, Rafael O. De Sá

Biology Faculty Publications

El orden Gymnophiona comprende anfibios ápodos, ciegos, de hábitos cavícolas y acuáticos. Como consecuencia de estos hábitos resulta muy difícil su colecta, lo cual redunda en un escaso conocimiento de la biología del orden y sobre las relaciones filogenéticas del mismo.

Una de las características más particulares del grupo se refiere a la modalidad reproductora, ya que aproximadamente el 50% de las especies presentan viviparidad y la totalidad de ellas tienen fecundación interna. Es por esta razón que el estudio del aparato reproductor ha sido encarado por diversos autores, en especial por Wake (1968, J. Morphol. 126: 291) con una …

Leptodactylus Podicipinus (Cope, 1862) Y Leptodactylus Chaquensis Cei, 1959 (Anura, Leptodactylidae), En El Uruguay, Raúl Vaz-Ferreira, Rafael O. De Sá, Federico Achaval, Annelise Gebrau

Biology Faculty Publications

En base al estudio de las colecciones obtenidas principalmente en excursiones a la zona NO de Uruguay con motivo del relevamiento faunístico del área de Salto Grande, se avanzó considerablemente en el conocimiento de la distribución de Leptodactylus podicipinus (Cope, 1862) y Leptodactylus chaquensis (Cei, 1950), en nuestro país.

Se cita el material estudiado y las observaciones de campo realizadas; se dan los caracteres generales de ambas especies, se describen los hábitats, y se tratan las características de las vocalizaciones.