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Supercomplex-Associated Cox26 Protein Binds To Cytochrome C Oxidase, Valentina Strecker, Zibirnisa Kadeer, Juliana Heidler, Cristina-Maria Cruciat, Heike Angerer, Heiko Giese, Kathy Pfeiffer, Rosemary A. Stuart, Ilka Wittig

Biological Sciences Faculty Research and Publications

Here we identified a hydrophobic 6.4 kDa protein, Cox26, as a novel component of yeast mitochondrial supercomplex comprising respiratory complexes III and IV. Multi-dimensional native and denaturing electrophoretic techniques were used to identify proteins interacting with Cox26. The majority of the Cox26 protein was found non-covalently bound to the complex IV moiety of the III–IV supercomplexes. A population of Cox26 was observed to exist in a disulfide bond partnership with the Cox2 subunit of complex IV. No pronounced growth phenotype for Cox26 deficiency was observed, indicating that Cox26 may not play a critical role in the COX enzymology, and we …