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Full-Text Articles in Life Sciences
Sequential Assembly Of Flagellar Radial Spokes, Dennis R. Diener, Pinfen Yang, Stefan Geimer, Douglas G. Cole, Winfield S. Sale, Joel L. Rosenbaum
Sequential Assembly Of Flagellar Radial Spokes, Dennis R. Diener, Pinfen Yang, Stefan Geimer, Douglas G. Cole, Winfield S. Sale, Joel L. Rosenbaum
Biological Sciences Faculty Research and Publications
The unicellular alga Chlamydomonas can assemble two 10 μm flagella in 1 h from proteins synthesized in the cell body. Targeting and transporting these proteins to the flagella are simplified by preassembly of macromolecular complexes in the cell body. Radial spokes are flagellar complexes that are partially assembled in the cell body before entering the flagella. On the axoneme, radial spokes are “T” shaped structures with a head of five proteins and a stalk of 18 proteins that sediment together at 20S. In the cell body, radial spokes are partially assembled; about half of the radial spoke proteins (RSPs) form …