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Nuclear Surveillance And Degradation Of Hypomodified Initiator TrnaMet In S. Cerevisiae, Sujatha Kadaba, Anna Krueger, Tamyra Trice, Annette M. Krecic, Alan G. Hinnebusch, James T. Anderson

Biological Sciences Faculty Research and Publications

The tRNA m¹A58 methyltransferase is composed of two subunits encoded by the essential genes TRM6 and TRM61 (formerly GCD10 and GCD14). The trm6-504 mutation results in a defective m¹A methyltransferase (Mtase) and a temperature-sensitive growth phenotype that is attributable to the absence of m¹A58 and consequential tRNA_i^Met instability. We used a genetic approach to identify the genes responsible for tRNA_i^Met degradation in trm6 cells. Three recessive extragenic mutations that suppress trm6-504 mutant phenotypes and restore hypomodified tRNA_i^Met to near normal levels were identified. The wild-type allele of …