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Biology

Biological Sciences Faculty Research and Publications

2004

Cold-Acclimation

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Cold Induction Of Earli1, A Putative Arabidopsis Lipid Transfer Protein, Is Light And Calcium Dependent, Jason A. Bubier, Michael Schläppi Jul 2004

Cold Induction Of Earli1, A Putative Arabidopsis Lipid Transfer Protein, Is Light And Calcium Dependent, Jason A. Bubier, Michael Schläppi

Biological Sciences Faculty Research and Publications

As sessile organisms, plants must adapt to their environment. One approach toward understanding this adaptation is to investigate environmental regulation of gene expression. Our focus is on the environmental regulation of EARLI1, which is activated by cold and long-day photoperiods. Cold activation of EARLI1 in short-day photoperiods is slow, requiring several hours at 4ºC to detect an increase in mRNA abundance. EARLI1 is not efficiently cold-activated in etiolated seedlings, suggesting that photomorphogenesis is necessary for its cold activation. Cold activation of EARLI1 is inhibited in the presence of the calcium channel blocker lanthanum chloride or the calcium chelator EGTA. Addition …