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Full-Text Articles in Life Sciences
Force-Velocity-Power And Force-Pca Relationships Of Human Soleus Fibers After 17 Days Of Bed Rest, Jeffrey J. Widrick, Kris M. Norenberg, C. A. Blaser, M. Karhanek, Jennifer J. Sherwood, Scott W. Trappe, Todd A. Trappe, David L. Costill, Robert H. Fitts
Force-Velocity-Power And Force-Pca Relationships Of Human Soleus Fibers After 17 Days Of Bed Rest, Jeffrey J. Widrick, Kris M. Norenberg, C. A. Blaser, M. Karhanek, Jennifer J. Sherwood, Scott W. Trappe, Todd A. Trappe, David L. Costill, Robert H. Fitts
Biological Sciences Faculty Research and Publications
Soleus muscle fibers from the rat display a reduction in peak power and Ca2+ sensitivity after hindlimb suspension. To examine human responses to non-weight bearing, we obtained soleus biopsies from eight adult men before and immediately after 17 days of bed rest (BR). Single chemically skinned fibers were mounted between a force transducer and a servo-controlled position motor and activated with maximal (isotonic properties) and/or submaximal (Ca2+ sensitivity) levels of free Ca2+. Gel electrophoresis indicated that all pre- and post-BR fibers expressed type I myosin heavy chain. Post-BR fibers obtained from one subject displayed increases in …
Position Effect Takes Precedence Over Target Sequence In Determination Of Adenine Methylation Patterns In The Nuclear Genome Of A Eukaryote, Tetrahymena Thermophila, Kathleen M. Karrer, Teresa A. Van Nuland
Position Effect Takes Precedence Over Target Sequence In Determination Of Adenine Methylation Patterns In The Nuclear Genome Of A Eukaryote, Tetrahymena Thermophila, Kathleen M. Karrer, Teresa A. Van Nuland
Biological Sciences Faculty Research and Publications
Approximately 0.8% of the adenine residues in the macronuclear DNA of the ciliated protozoan Tetrahymena thermophila are modified to N6-methyladenine. DNA methylation is site specific and the pattern of methylation is constant between clonal cell lines. In vivo, modification of adenine residues appears to occur exclusively in the sequence 5′-NAT-3′, but no consensus sequence for modified sites has been found. In this study, DNA fragments containing a site that is uniformly methylated on the 50 copies of the macronuclear chromosome were cloned into the extrachromosomal rDNA. In the novel location on the rDNA minichromosome, the site was …
Concerted Formation Of Macromolecular Suppressor-Mutator Transposition Complexes, Ramesh Raina, Michael Schläppi, Balasulojini Karunanandaa, Adam Elhofy, Nina V. Fedoroff
Concerted Formation Of Macromolecular Suppressor-Mutator Transposition Complexes, Ramesh Raina, Michael Schläppi, Balasulojini Karunanandaa, Adam Elhofy, Nina V. Fedoroff
Biological Sciences Faculty Research and Publications
Transposition of the maize Suppressor-mutator (Spm) transposon requires two element-encoded proteins, TnpA and TnpD. Although there are multiple TnpA binding sites near each element end, binding of TnpA to DNA is not cooperative, and the binding affinity is not markedly affected by the number of binding sites per DNA fragment. However, intermolecular complexes form cooperatively between DNA fragments with three or more TnpA binding sites. TnpD, itself not a sequence-specific DNA-binding protein, binds to TnpA and stabilizes the TnpA-DNA complex. The high redundancy of TnpA binding sites at both element ends and the protein-protein interactions between DNA-bound TnpA …
Myosin Heavy Chain Isoforms And Dynamic Contractile Properties: Skeletal Versus Smooth Muscle, Thomas J. Eddinger
Myosin Heavy Chain Isoforms And Dynamic Contractile Properties: Skeletal Versus Smooth Muscle, Thomas J. Eddinger
Biological Sciences Faculty Research and Publications
Myosin, one of the primary contractile muscle proteins, displays molecular, enzymatic, structural, functional and regulatory variability. This variability has been shown to account for a significant amount of the functional uniqueness of skeletal and smooth muscle. However, the universal generation of force and/or shortening by these two muscle types belies the ever-increasing number of known distinct differences that bring this about. Thus, the notion that the functional roles of skeletal and smooth muscle, their development and regulation, all appear to be uniquely applicable for their physiological purpose no longer appears heretical. This manuscript presents a cursory overview of the numerous …