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Full-Text Articles in Life Sciences
Arachidonic Acid As A Possible Negative Feedback Inhibitor Of Nicotinic Acetylcholine Receptors On Neurons, Sukumar Vijayaraghavan, Bo Huang, Edward M. Blumenthal, Darwin K. Berg
Arachidonic Acid As A Possible Negative Feedback Inhibitor Of Nicotinic Acetylcholine Receptors On Neurons, Sukumar Vijayaraghavan, Bo Huang, Edward M. Blumenthal, Darwin K. Berg
Biological Sciences Faculty Research and Publications
Neuronal acetylcholine receptors, being highly permeable to calcium, are likely to regulate calcium-dependent events in neurons. Arachidonic acid is a membrane-permeant second messenger that can be released from membrane phospholipids by phospholipases in a calcium-dependent manner. We show here that activation of neuronal acetylcholine receptors triggers release of 3H-arachidonic acid in a calcium-dependent manner from neurons preloaded with the fatty acid. Moreover, low concentrations of arachidonic acid reversibly inhibit the receptors and act most efficiently on receptors likely to have the highest permeability to calcium, namely receptors containing α7 subunits. Low concentrations of arachidonic acid also reversibly inhibit α7- …
Quantitative Analysis Of Electrotonic Structure And Membrane Properties Of Nmda-Activated Lamprey Spinal Neurons, C. R. Murphey, L. E. Moore, James T. Buchanan
Quantitative Analysis Of Electrotonic Structure And Membrane Properties Of Nmda-Activated Lamprey Spinal Neurons, C. R. Murphey, L. E. Moore, James T. Buchanan
Biological Sciences Faculty Research and Publications
Parameter optimization methods were used to quantitatively analyze frequency-domain-voltage-clamp data of NMDA-activated lamprey spinal neurons simultaneously over a wide range of membrane potentials. A neuronal cable model was used to explicitly take into account receptors located on the dendritic trees. The driving point membrane admittance was measured from the cell soma in response to a Fourier synthesized point voltage clamp stimulus. The data were fitted to an equivalent cable model consisting of a single lumped soma compartment coupled resistively to a series of equal dendritic compartments. The model contains voltage-dependent NMDA sensitive (INMDA), slow potassium (IK …
Lipopolysaccharide Core Structures In Rhizobium Etli And Mutants Deficient In O-Antigen, Russell W. Carlson, Bradley Reuhs, Tong-Bin Chen, U. Ramadas Bhat, K. Dale Noel
Lipopolysaccharide Core Structures In Rhizobium Etli And Mutants Deficient In O-Antigen, Russell W. Carlson, Bradley Reuhs, Tong-Bin Chen, U. Ramadas Bhat, K. Dale Noel
Biological Sciences Faculty Research and Publications
Lipopolysaccharide (LPS) is a major component of the bacterial outer membrane, and for Rhizobium spp. has been shown to play a critical role in the establishment of an effective nitrogen-fixing symbiosis with a legume host. Many genes required for O-chain polysaccharide synthesis are in the lps α region of the CE3 genome; this region may also carry lps genes required for core oligosaccharide synthesis. The LPSs from several strains mutated in the α region were isolated, and their mild acid released oligosaccharides, purified by high performance anion-exchange chromatography, were characterized by electrospray- and fast atom bombardment-mass spectrometry, NMR, and …
Nitrous Oxide: Mechanism Of Its Antinociceptive Action, Raymond M. Quock, Linda K. Vaughn
Nitrous Oxide: Mechanism Of Its Antinociceptive Action, Raymond M. Quock, Linda K. Vaughn
Biological Sciences Faculty Research and Publications
Nitrous oxide (N2O) is an anesthetic gas known to produce an analgesic effect at sub-anesthetic concentrations. This analgesic property of N2O can be clinically exploited in a broad range of conditions where pain relief is indicated. The mechanism of this analgesic effect was long thought to be nonspecific in nature, but a landmark study by Berkowitz and others in 1976 first implicated an opioid mechanism of action, possibly via N2O-stimulated neuronal release of endogenous opioid peptides to activate opioid receptors. N2O-induced release of opioid peptide has been demonstrated in both in vivo …
Localization And Interaction Of N-Methyl-D-Aspartate And Non-N-Methyl-D-Aspartate Receptors Of Lamprey Spinal Neurons, L. E. Moore, James T. Buchanan, C. R. Murphey
Localization And Interaction Of N-Methyl-D-Aspartate And Non-N-Methyl-D-Aspartate Receptors Of Lamprey Spinal Neurons, L. E. Moore, James T. Buchanan, C. R. Murphey
Biological Sciences Faculty Research and Publications
Small volumes of N-Methyl-D-Aspartate (NMDA) and non-NMDA excitatory amino acid receptor agonists were applied to localized regions of the dendritic trees of lamprey spinal neurons along their medial-lateral axis to obtain a spatial map of glutamate receptor distribution. Voltage clamp and frequency domain methods were used to obtain quantitative kinetic data of the voltage dependent ionic channels located both on the soma and on highly branched dendritic membranes. Pressure pulses of NMDA applied to the most peripheral regions of the dendritic tree elicited large somatic impedance increases, indicating that the most peripheral dendrites are well supplied with NMDA receptors. Experiments …