Life Sciences Commons^™

Analysis Of Enzyme, Carbohydrate And Mineral Distribution In The Foot Of Abalone Shellfish., Leanne F. Harris, Nuala O'Byrne-Ring, Helen Lambkin

Articles

No abstract provided.

Isolation And Characterization Of Toxin A-Negative, Toxin B-Positive Clostridium Difficile In Dublin, Ireland, Denise Drudy, N. Harnedy, S. Fanning, R. O’Mahony, L. Kyne

Articles

Clostridium difficile is a major cause of infectious diarrhoea in hospitalised patients. Most pathogenic C. difficile strains produce two toxins, A and B; however, clinically relevant toxin A-negative, toxin Bpositive (A– B+ ) strains of C. difficile that cause diarrhoea and colitis in humans have been isolated worldwide. The aims of this study were to isolate and characterise A– B+ strains from two university hospitals in Dublin, Ireland. Samples positive for C. difficile were identified daily by review of ELISA results and were cultured on selective media. Following culture, toxin-specific immunoassays, IMR-90 cytotoxicity assays and PCR were used to analyse …

Comparative Phylogenomics Of Clostridium Difficile Reveals Clade Specificity And Microevolution Of Hypervirulent Strains., Denise Drudy

Articles

No abstract provided.

Characterisation Of Cell Types In Abalone (Haliotis Spp.) Tissues Using Immunohistochemical Techniques, Leanne Harris, Nuala O'Byrne-Ring, Helen Lambkin

Articles

The increasing popularity of abalone as a seafood delicacy has led to the rapid development of abalone aquaculture worldwide. The commercial and economic importance of this industry has resulted in an increasing interest in the biology of this particular shellfish genus. In this study we focus on the identification of structural, functional and proliferative proteins in two species of abalone shellfish, Haliotis discus hannai and Haliotis tuberculata. Monoclonal and polyclonal antibodies that react with proteins in vertebrate and invertebrate tissues were selected and applied to abalone tissues. Cross sections of whole animals were analysed using avidin–biotin immunoperoxidase staining protocols. In …

High-Level Resistance To Moxifloxacin And Gatifloxacin Associated With A Novel Mutation In Gyrb In Toxin-A-Negative, Toxin-B-Positive Clostridium Difficile, Denise Drudy, T. Quinn, R. O'Mahony, L. Kyne, P. O'Gaora, S. Fanning

Articles

OBJECTIVES:

To determine the mechanism of high-level resistance to fluoroquinolone antimicrobials in toxin-A-negative, toxin-B-positive (A- B+) Clostridium difficile isolates.

METHODS:

Following culture 16-23S PCR ribotyping was used to determine genomic relationships between A- B+ C. difficile isolates. Antimicrobial susceptibilities were determined using Etests in the presence and absence of the efflux pump inhibitors reserpine (20 microg/mL), L-phenylalanine-L-arginine-beta-naphthylamide (PAbetaN; 20 microg/mL) and verapamil (100 microg/mL). Genomic regions including the quinolone-resistance-determining-region (QRDR) of gyrA and gyrB were amplified and characterized.

RESULTS:

PCR ribotyping profiles identified one major cluster of A- B+ C. difficile, universally resistant to the fluoroquinolones tested (ofloxacin, ciprofloxacin, levofloxacin, …