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Regulation Of Mitotic Progression By Btf And Trap150, Divya Cheedu
Regulation Of Mitotic Progression By Btf And Trap150, Divya Cheedu
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Serine-arginine-rich (SR) or SR-like splicing factors interact with exon junction complex proteins during pre-mRNA processing to promote mRNA packaging into mature messenger ribonucleoproteins (mRNPs) and to dictate mRNA stability, nuclear export, and translation. In this thesis, I examined if depletion of two homologous non-classical serine-arginine-rich (SR) splicing factors, Btf (BCLAF1) and TRAP150, impacts regulation of cell cycle regulator transcripts and mitosis. Previous work showed that depletion of these proteins by RNAi causes mitotic defects including chromosome misalignment in metaphase. However, since Btf and/or TRAP150 did not co-localize with mitotic structures during mitosis, I hypothesized that Btf and/or TRAP150 depletion affect …