Life Sciences Commons^™

Occurrence And Distribution Of The Freshwater Amphipodsgammarus Pseudolimnaeus And Gammarus Fasciatus Insoutheastern Virginia, Randolph Chambers

Arts & Sciences Articles

The freshwater amphipod, Gammarus fasciatus, and a population that keys to Gammarus pseudolimnaeusare broadly sympatric in southeastern Virginia. By documenting the successful formation of pre-copulatory mate-guarding pairs between individuals collected from Virginia and New York, we confirmed the occurrence of a G. pseudolimnaeus population strongly disjunct from the previously described range in the Mississippi and St. Lawrence River drainage basins. Gammarus pseudolimnaeus appears restricted to high-quality, spring-fed streams that occur at low density across an increasingly fragmented natural landscape in Virginia. Gammarus fasciatus, however, occurs in lakes and streams of developed landscapes that typically are more degraded, …

Dissolved And Particulate Organic Carbon Fluxes From An Agricultural Watershed During Consecutive Tropical Storms, Randolph Chambers

Arts & Sciences Articles

Low‐frequency high‐magnitude hydrologic events mobilize a disproportionate amount of dissolved organic carbon (DOC) from watersheds, but few studies measure the role of extreme storms in exporting organic carbon from croplands. We use high‐resolution measurements of storm runoff to quantify DOC and particulate organic carbon (POC) fluxes from an agricultural field during consecutive tropical storms that delivered 41 cm of rainfall to the Virginia Coastal Plain. Over a 2 week period, we measured exports of 22 kg DOC ha−1 and 11.3 kg POC ha−1. Ultraviolet absorbance measurements indicate that the aromatic DOC fraction systematically increased as plant‐derived aliphatic carbon was depleted …

Photochemical And Microbial Alteration Of Dissolved Organic Matter In Temperate Headwater Streams Associated With Different Land Use, Randolph Chambers

Arts & Sciences Articles

Photochemical and microbial transformations of DOM were evaluated in headwater streams draining forested and human‐modified lands (pasture, cropland, and urban development) by laboratory incubations. Changes in DOC concentrations, DOC isotopic signatures, and DOM fluorescence properties were measured to assess the amounts, sources, ages, and properties of reactive and refractory DOM under the influence of photochemistry and/or bacteria. DOC in streams draining forest‐dominated watersheds was more photoreactive than in streams draining mostly human‐modified watersheds, possibly due to greater contributions of terrestrial plant‐derived DOC and lower amounts of prior light exposure in forested streams. Overall, the percentage of photoreactive DOC in stream …

Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher

Arts & Sciences Articles

Homogenization by bead beating is a fast and efficient way to release DNA, RNA, proteins, and metabolites from budding yeast cells, which are notoriously hard to disrupt. Here we describe the use of a bead mill homogenizer for the extraction of proteins into buffers optimized to maintain the functions, interactions and post-translational modifications of proteins. Logarithmically growing cells expressing the protein of interest are grown in a liquid …

Habitat And Fish Population Dynamics: Advancing Stock Assessments Of Highly Migratory Species, Patrick D. Lynch

Dissertations, Theses, and Masters Projects

Fisheries sustainability is inherently linked to an understanding of the population-level effects of fishing. With an accurate characterization of historical fish and fishery dynamics, management agencies are more equipped to create regulations that sustain fishery resources over the long term. The overarching goal of this dissertation is to contribute to the advancement of fisheries stock assessment and promote resource sustainability. My research focused on highly migratory species (HMS), particularly fishes that reside in the open ocean. These species constitute some of the highest valued global fisheries; however, numerous factors compromise HMS stock assessment and management. These challenges are fully described …

Integrated Carbon Budget Models For The Everglades Terrestrial-Coastal-Oceanic Gradient: Current Status And Needs For Inter-Site Comparisons., Randolph Chambers

Arts & Sciences Articles

Recent studies suggest that coastal ecosystems can bury significantly more C than tropical forests, indicating that continued coastal development and exposure to sea level rise and storms will have global biogeochemical consequences. e Florida Coastal Everglades Long Term Ecological Research (FCE LTER) site provides an excellent subtropical system for examining carbon (C) balance because of its exposure to historical changes in freshwater distribution and sea level rise and its history of significant long-term carbon-cycling studies. FCE LTER scientists used net ecosystem C balance and net ecosystem exchange data to estimate C budgets

for riverine mangrove, freshwater marsh, and seagrass meadows, …