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Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien
Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien
WWU Graduate School Collection
The pinto abalone, Haliotis kamtschatkana, is an ecosystem engineer with cultural and ecological significance. Due to the dramatic 98% decline in populations, a ten-year recovery plan was enacted to bring levels of pinto abalone back from the brink of extirpation in the San Juan Archipelago, Washington. The focus of this study was to assist restoration efforts in creating self-sustaining populations by developing methods of cryopreservation for male pinto abalone sperm. My study evaluated three commonly used cryoprotectants, a series of freeze/thaw temperatures, and developed methods of quality assessment specific to pinto abalone. Animals were provided and cared for by …