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Full-Text Articles in Life Sciences

Spatiotemporal Diversity Of Alpine Snow Algae Communities In The Pacific Northwest, Rachael C. Mallon Jan 2019

Spatiotemporal Diversity Of Alpine Snow Algae Communities In The Pacific Northwest, Rachael C. Mallon

WWU Graduate School Collection

Snow algae are the dominant primary producers of snowy alpine environments and have recently been thrust into the public spotlight for contributing to glacial melt by decreasing snow albedo. These microbial communities are subject to extreme temperature regimes, high irradiance, low nutrient levels, and freeze-thaw cycles on daily, seasonal, and even long-term climatological changes. Although snow algae have been described on every continent, the spatiotemporal diversity of snow algae communities across snowy habitats has not been addressed. The natural geography and climate of the Pacific Northwest provides diverse snowy alpine ecosystems to study the effects of latitude, elevation, and precipitation …


Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien Jan 2019

Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien

WWU Graduate School Collection

The pinto abalone, Haliotis kamtschatkana, is an ecosystem engineer with cultural and ecological significance. Due to the dramatic 98% decline in populations, a ten-year recovery plan was enacted to bring levels of pinto abalone back from the brink of extirpation in the San Juan Archipelago, Washington. The focus of this study was to assist restoration efforts in creating self-sustaining populations by developing methods of cryopreservation for male pinto abalone sperm. My study evaluated three commonly used cryoprotectants, a series of freeze/thaw temperatures, and developed methods of quality assessment specific to pinto abalone. Animals were provided and cared for by …


Functional Analysis Of Cyc2 In Microbial Mat Communities Of Mariana Arc And Back-Arc Hydrothermal Vents, Christina A. Turner Jan 2019

Functional Analysis Of Cyc2 In Microbial Mat Communities Of Mariana Arc And Back-Arc Hydrothermal Vents, Christina A. Turner

WWU Graduate School Collection

The primary production by Zetaproteobacteria at iron-rich vents of the Mariana Arc and back-arc supports high microbial diversity. In microaerophilic circumneutral pH, Zetaproteobacteria are able to produce energy by oxidizing ferrous iron (Fe2+) from the vent effluent with the presence of a key gene involved in their iron-oxidation, Cyc2, a fused cytochrome-porin. These genes are found in all Zetaproteobacteria known to date. This study combines molecular quantitative techniques with metagenomic analyses to enhance our confidence in gene quantification from environmental samples. The occurrence of cyc2 was quantified from microbial mat communities at NW Eifuku, NW Rota-1, Snail and …


Optimizing Abalone Outplant Strategies Using Larval Haliotis Kamtschatkana In The San Juan Archipelago, Washington, Katherine Mills-Orcutt Jan 2019

Optimizing Abalone Outplant Strategies Using Larval Haliotis Kamtschatkana In The San Juan Archipelago, Washington, Katherine Mills-Orcutt

WWU Graduate School Collection

Prior studies testing the use of Haliotis kamtschatkana larvae as a means of stock enhancement in the San Juan archipelago have shown no success in inducing larvae to settle in densities required for successful reproduction. I conducted an experiment to test the effectiveness of new methods for outplanting larval abalone in hopes of creating a protocol Puget Sound Restoration Fund could use to restore wild populations. Using two sites within the San Juan archipelago, I outplanted abalone into pre-constructed larval abalone modules (LAMs) and sampled over four months, testing retention strategies. Three types of LAMs were used at each site …