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Cupid’S Arrow: A Tale Of A Complex And Dynamic Protein, Nadine Tietz
Cupid’S Arrow: A Tale Of A Complex And Dynamic Protein, Nadine Tietz
WWU Honors College Senior Projects
My capstone project sought to investigate the degree to which ARO4 gene expression is influenced by local vs distant genetic variants. This involved engineering allele-swap strains of ARO4 tagged with a co-expressing fluorescent protein. I then compared protein expression between each strain's genotype which provided an estimate of the proportion of the overall phenotypic variation that can be explained by genetic variation between the underlying genomes.
From my experiment I was able to conclude that ARO4 protein expression was complex and dynamic as both strain background and allele had genetic influences on ARO4 protein expression. In addition, not only did …