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Full-Text Articles in Life Sciences
The Effect Of Wee1 On Mitosis In The Spb Mutant, David Leroy
The Effect Of Wee1 On Mitosis In The Spb Mutant, David Leroy
Honors Theses
The speedbump mutant has been identified as an early arrest mutant, becoming apparent as soon as 80% epiboly (8.5 hours of development). This mutant has a mutation in wee1, which is a protein regulating entry into mitosis ensuring the completion of DNA replication before mitosis. The lack of this protein in speedbump allows for early entry into mitosis resulting in damage to the chromosome. Here, we investigate two questions: if mitosis in the speedbump mutant phenotype is more frequent than wild-type phenotype, and, if so, is there a phase in which the mutant phenotype favors. We used phosphorylated histone H3 …
Physiology And Genetics Shape The Microbiome Of A Seabird Species (Oceanodroma Leucorhoa) More Than Environmental And Social Factors, Douglas Steven Pearce
Physiology And Genetics Shape The Microbiome Of A Seabird Species (Oceanodroma Leucorhoa) More Than Environmental And Social Factors, Douglas Steven Pearce
Masters Theses
The microbiome provides multiple benefits to animal hosts that can profoundly impact health and behavior. Microbiomes are well-characterized in humans and animals in controlled settings, yet assessments of wild bird microbial communities remain vastly understudied. This study examines the multiple factors that affect the microbiome of a burrow-nesting Procellariiform bird species, Leach’s storm-petrel. 16S rRNA-based Illumina Mi-Seq analyses are used to assess the composition and structure of bird and burrow-associated bacterial communities. Results indicate that sex and skin site contribute to bird-associated bacterial community variation, and MHC heterozygosity impacts these bacterial assemblages in a sex and site-specific manner, potentially having …
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Honors Theses
A Klebsiella pneumoniae bacteriophage was isolated from waste water and purified over multiple rounds of plaque removals and serial titrations. This bacteriophage was imaged in a transmission electron microscope and then tested with selected antibiotics for effectiveness in reducing optical density of K. pneumoniae colonies. The phage was shown to have an increased effectiveness in reducing the optical density when used together with an antibiotic that the bacteria was susceptible to.
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Honors Theses
Consumption of a prominent synthetic cathinone known as mephedrone (4-methylmethcathinone) has become a popular alternative to club drugs such as ecstasy, cocaine, and methamphetamine within the past decade. The pharmacological mechanisms that contribute to its subjective effects have yet to be fully characterized and are thus warranted for investigation. The present study employed drug discrimination methods to train eight male Sprague-Dawley rats to discriminate injections of 3.0 mg/kg mephedrone from saline. Various doses of mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), d-amphetamine (AMPH), cocaine, (+)-methamphetamine (METH), and 3,4-methylenedioxy-methamphetamine (MDMA) were investigated for their ability to substitute for the stimulus cues of the training …
Injury-Induced Neuronal Turnover With Zinc Sulfate Affects Cilitated Olfactory Sensory Neurons More Than Microvillous Olfactory Sensory Neurons In The Adult Zebrafish, James Hentig
Honors Theses
Zinc sulfate is a known olfactory toxicant, although its effects on the olfactory epithelium of zebrafish have not been examined. Previous observations utilizing Triton X-100 found selective retention of some olfactory sensory neurons and loss of others. Based on those findings, we hypothesized that the retention of the ability to sense amino acids, detected by microvillous olfactory sensory neurons, and loss of the ability to perceive bile salts, detected by ciliated olfactory sensory neurons, is a universal response to chemical ablation. Fish were treated with zinc sulfate and survived for 2, 5, 10 and 14 days post treatment and processed …